Rabbit LYN Polyclonal Antibody | anti-LYN antibody

LYN Polyclonal Antibody

Cat. Num. AAA127529

• Gene Names: LYN; JTK8; p53Lyn; p56Lyn
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: LYN; Polyclonal Antibody; LYN Polyclonal Antibody; Lyn: FLJ26625; JTK8; anti-LYN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 512

• Applicable Applications for anti-LYN antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human LYN
• Calculated Molecular Weight: 59kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using LYN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Related Product Information for anti-LYN antibody

Lyn, one of the Src family members, is predominantly expressed in hematopoietic cells (1). Two tyrosine residues have been reported to play a crucial role in the regulation of protein tyrosine kinases of the Src family. Autophosphorylation of Tyr396 (equivalent to Tyr416 of Src), located in the catalytic domain, correlates with enzyme activation. Csk-mediated phosphorylation of the carboxy-terminal Tyr507 (equivalent to Tyr527 of Src) inactivates the kinase. Tyrosine phosphorylation and activation of Lyn occurs upon association with cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (2-4). Studies using knockout mice have shown that the net effect of Lyn deficiency is to render B cells hypersensitive to BCR stimulation (5-7), suggesting that the most critical role for Lyn in vivo is in the down-regulation of B cell responses. Lyn is also involved in controlling the migration and development of specific B cell populations (8).

Product Categories/Family for anti-LYN antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 125480
• NCBI GeneID: 4067
• UniProt Accession #: P07948; A0AVQ5
• Molecular Weight: 512
• NCBI Official Full Name: Tyrosine-protein kinase Lyn
• NCBI Official Synonym Full Names: LYN proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: LYN
• NCBI Official Synonym Symbols: JTK8; p53Lyn; p56Lyn
• NCBI Protein Information: tyrosine-protein kinase Lyn; lck/Yes-related novel protein tyrosine kinase; v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• UniProt Protein Name: Tyrosine-protein kinase Lyn
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: LYN
• UniProt Synonym Gene Names: JTK8
• UniProt Entry Name: LYN_HUMAN

NCBI Description

This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

LYN: a tyrosine kinase of the Src family that plays a role in B cell antigen-receptor signaling.

Protein type: Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Oncoprotein; EC 2.7.10.2; Kinase, protein; TK group; Src family

Chromosomal Location of Human Ortholog: 8q13

Cellular Component: Golgi apparatus; mitochondrial crista; extrinsic to internal side of plasma membrane; mast cell granule; perinuclear region of cytoplasm; cytoplasm; postsynaptic density; plasma membrane; mitochondrial intermembrane space; cytosol; nucleus; lipid raft

Molecular Function: integrin binding; protein binding; enzyme binding; protein-tyrosine kinase activity; ubiquitin protein ligase binding; platelet-derived growth factor receptor binding; non-membrane spanning protein tyrosine kinase activity; phosphoprotein binding; receptor signaling protein tyrosine kinase activity; glycosphingolipid binding; SH3 domain binding; ATP binding; receptor binding

Biological Process: regulation of mast cell degranulation; negative regulation of MAP kinase activity; central nervous system development; viral reproduction; response to toxin; positive regulation of B cell receptor signaling pathway; response to organic cyclic substance; protein amino acid phosphorylation; response to carbohydrate stimulus; regulation of B cell apoptosis; B cell receptor signaling pathway; erythrocyte differentiation; oligodendrocyte development; tolerance induction to self antigen; response to drug; platelet activation; negative regulation of immune response; response to sterol depletion; immune response-regulating cell surface receptor signaling pathway; response to amino acid stimulus; regulation of erythrocyte differentiation; histamine secretion by mast cell; T cell costimulation; leukocyte migration; cytokine secretion; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of mast cell activation; axon guidance; regulation of cytokine secretion; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; response to hormone stimulus; negative regulation of B cell proliferation; signal transduction; response to insulin stimulus; regulation of cytokine production; negative regulation of cell proliferation; regulation of cell adhesion mediated by integrin; platelet degranulation; negative regulation of protein amino acid phosphorylation; B cell homeostasis; positive regulation of cell proliferation; ephrin receptor signaling pathway; lipopolysaccharide-mediated signaling pathway; Fc receptor mediated inhibitory signaling pathway; Fc receptor mediated stimulatory signaling pathway; response to axon injury; inflammatory response; regulation of B cell receptor signaling pathway; regulation of protein amino acid phosphorylation; negative regulation of myeloid leukocyte differentiation; negative regulation of toll-like receptor 2 signaling pathway; positive regulation of phosphoinositide 3-kinase activity; positive regulation of cell motility; regulation of release of sequestered calcium ion into cytosol; positive regulation of tyrosine phosphorylation of STAT protein; regulation of inflammatory response; negative regulation of toll-like receptor 4 signaling pathway; cellular response to extracellular stimulus; innate immune response; blood coagulation; response to DNA damage stimulus; positive regulation of cell migration