PDK1 cdna clone
PDK1 cDNA Clone
NCBI and Uniprot Product Information
NCBI Description
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]
Uniprot Description
PDHK1: an ubiquitously expressed, atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK1 is detected in heart, pancreatic islets, and skeletal muscles. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. PDHK1 is a direct HIF-1 target gene. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA) and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.
Protein type: Kinase, protein; Protein kinase, atypical; Mitochondrial; EC 2.7.11.2; ATYPICAL group; PDHK family
Chromosomal Location of Human Ortholog: 2q31.1
Cellular Component: mitochondrial matrix; mitochondrion
Molecular Function: protein binding; protein kinase activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity
Biological Process: cell proliferation; induction of apoptosis by oxidative stress; regulation of acetyl-CoA biosynthetic process from pyruvate
Research Articles on PDK1
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Product Notes
The PDK1 pdk1 (Catalog #AAA1268943) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: atgaggctgg cgcggctgct tcgcggagcc gccttggccg gcccgggccc ggggctgcgc gccgccggct tcagccgcag cttcagctcg gactcgggct ccagcccggc gtccgagcgc ggcgttccgg gccaggtgga cttctacgcg cgcttctcgc cgtccccgct ctccatgaag cagttcctgg acttcggatc agtgaatgct tgtgaaaaga cctcatttat gtttctgcgg caagagttgc ctgtcagact ggcaaatata atgaaagaaa taagtctcct tccagataat cttctcagga caccatccgt tcaattggta caaagctggt atatccagag tcttcaggag cttcttgatt ttaaggacaa aagtgctgag gatgctaaag ctatttatga ctttacagat actgtgatac ggatcagaaa ccgacacaat gatgtcattc ccacaatggc ccagggtgtg attgaataca aggagagctt tggggtggat cctgtcacca gccagaatgt tcagtacttt ttggatcgat tctacatgag tcgcatttca attagaatgt tactcaatca gcactcttta ttgtttggtg gaaaaggcaa aggaagtcca tctcatcgaa aacacattgg aagcataaat ccaaactgca atgtacttga agttattaaa gatggctatg aaaatgctag gcgtctgtgt gatttgtatt atattaactc tcccgaacta gaacttgaag aactaaatgc aaaatcacca ggacagccaa tacaagtggt ttatgtacca tcccatctct atcacatggt gtttgaactt ttcaagaatg caatgagagc cactatggaa caccatgcca acagaggtgt ttacccccct attcaagttc atgtcacgct gggtaatgag gatttgactg tgaagatgag tgaccgagga ggtggcgttc ctttgaggaa aattgacaga cttttcaact acatgtattc aactgcacca agacctcgtg ttgagacctc ccgcgcagtg cctctggctg gttttggtta tggattgccc atatcacgtc tttacgcaca atacttccaa ggagacctga agctgtattc cctagagggt tacgggacag atgcagttat ctacattaag gctctgtcaa cagactcaat agaaagactc ccagtgtata acaaagctgc ctggaagcat tacaacacca accacgaggc tgatgactgg tgcgtcccca gcagagaacc caaagacatg acgacgttcc gcagtgccta g. It is sometimes possible for the material contained within the vial of "PDK1, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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