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WB (Western Blot)
(Western blot of anti-CD44 mAb against HeLa (1) and HUVE-12(2) cell lysate.)
Mouse anti-Human, Mouse CD44 Monoclonal Antibody | anti-CD44 antibody
Mouse Monoclonal [clone 8E2F3] (IgG1) to Human CD44
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Ascites
Synonyms
CD44; Monoclonal Antibody; Mouse Monoclonal [clone 8E2F3] (IgG1) to Human CD44; Anti-CD44 Antibody (clone 8E2F3) IHC-plus; CDW44; CD44 antigen; Cell surface glycoprotein CD44; CSPG8; ECMR-III; Epican; HUTCH-I; LHR; MDU2; MC56; MIC4; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; PGP-I; Pgp1; Phagocytic glycoprotein I; MDU3; CD44R; HCELL; Human CD44; anti-CD44 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8E2F3
Specificity
human CD44
Purity/Purification
Ascites
Form/Format
Ascites fluid, 0.03% sodium azide
Sequence Length
742
Applicable Applications for anti-CD44 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
ELISA (1:10000), Flo (1:200 - 1:400), IF (1:200 - 1:1000), IHC-P (1:50), WB (1:500 - 1:2000)
Target Species
Human
Immunogen Description
Purified recombinant fragment of human CD44 (628-699) expressed in E Coli.
Immunogen
CD44 antibody was raised against purified recombinant fragment of human CD44 (628-699) expressed in E Coli.
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
WB (Western Blot)
(Western blot of anti-CD44 mAb against HeLa (1) and HUVE-12(2) cell lysate.)
WB (Western Blot)
(Western blot of anti-CD44 mAb against HeLa (1) and HUVE-12(2) cell lysate.)
WB (Western Blot)
(Western blot of anti-CD44 mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).)
WB (Western Blot)
(Western blot of anti-CD44 mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).)
WB (Western Blot)
(Confocal analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.)
WB (Western Blot)
(Confocal analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.)
FCM (Flow Cytometry)
(Flow cytometry of HeLa cells using anti-CD44 mAb (right) and negative control (left).)
FCM (Flow Cytometry)
(Flow cytometry of HeLa cells using anti-CD44 mAb (right) and negative control (left).)
ICC (Immunocytochemistry)
(Confocal immunofluorescence of methanol-fixed A431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.)
ICC (Immunocytochemistry)
(Confocal immunofluorescence of methanol-fixed A431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.)
IF (Immunofluorescence)
(Confocal immunofluorescence of PANC-1 cells using anti-CD44 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
IF (Immunofluorescence)
(Confocal immunofluorescence of PANC-1 cells using anti-CD44 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
IHC (Immunohistochemistry)
(Anti-CD44 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
IHC (Immunohistochemistry)
(Anti-CD44 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
Related Product Information for anti-CD44 antibody
CD44 is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,635 Da
NCBI Official Full Name
CD44 antigen isoform 1
NCBI Official Synonym Full Names
CD44 molecule (Indian blood group)
NCBI Official Symbol
CD44
NCBI Official Synonym Symbols
IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
NCBI Protein Information
CD44 antigen; epican; Hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; heparan sulfate proteoglycan; cell surface glycoprotein CD44; extracellular matrix receptor III; chondroitin sulfate proteoglycan 8; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; homing function and Indian blood group system
UniProt Protein Name
CD44 antigen
UniProt Gene Name
CD44
UniProt Synonym Gene Names
LHR; MDU2; MDU3; MIC4; ECMR-III; PGP-1; PGP-I
UniProt Entry Name
CD44_HUMAN
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Product Notes
The CD44 cd44 (Catalog #AAA12358) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 8E2F3] (IgG1) to Human CD44 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD44 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA). ELISA (1:10000), Flo (1:200 - 1:400), IF (1:200 - 1:1000), IHC-P (1:50), WB (1:500 - 1:2000). Researchers should empirically determine the suitability of the CD44 cd44 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD44, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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