Sulfur carrier protein ThiS adenylyltransferase (thiF) Recombinant Protein | thiF recombinant protein
Recombinant Escherichia coli Sulfur carrier protein ThiS adenylyltransferase (thiF)
Gene Names
thiF; ECK3984; JW3956; thiA
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Sulfur carrier protein ThiS adenylyltransferase (thiF); Recombinant Escherichia coli Sulfur carrier protein ThiS adenylyltransferase (thiF); thiF recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-251, full length protein
Sequence
MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAALYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTALQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMVLTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGELRLFDGKSSQWRSLALRRASGCPVCGGSNADPV
Sequence Length
251
Species
Escherichia coli (strain K12)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,970 Da
NCBI Official Full Name
adenylyltransferase, modifies ThiS C-terminus
NCBI Official Symbol
thiF
NCBI Official Synonym Symbols
ECK3984; JW3956; thiA
NCBI Protein Information
adenylyltransferase, modifies ThiS C-terminus
UniProt Protein Name
Sulfur carrier protein ThiS adenylyltransferase
Protein Family
UniProt Gene Name
thiF
NCBI Description
The ThiF sequence has been confirmed using MS/MS, however since use of the start codon was forced during cloning, this is not a verification of the start site: the measured mass of the intact protein is 26970.2 indicating that the initial Met is not removed (Kelleher, 1998). [More information is available at EcoGene: EG11587]. The ThiF protein catalyzes the adenylation of the ThiS protein as part of the formation of the thiazole moiety during thiamin biosynthesis . [More information is available at EcoCyc: EG11587].
Uniprot Description
Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS.
Research Articles on thiF
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Product Notes
The thiF thif (Catalog #AAA1225636) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-251, full length protein. The amino acid sequence is listed below: MNDRDFMRYS RQILLDDIAL DGQQKLLDSQ VLIIGLGGLG TPAALYLAGA GVGTLVLADD DDVHLSNLQR QILFTTEDID RPKSQVSQQR LTQLNPDIQL TALQQRLTGE ALKDAVARAD VVLDCTDNMA TRQEINAACV ALNTPLITAS AVGFGGQLMV LTPPWEQGCY RCLWPDNQEP ERNCRTAGVV GPVVGVMGTL QALEAIKLLS GIETPAGELR LFDGKSSQWR SLALRRASGC PVCGGSNADP V. It is sometimes possible for the material contained within the vial of "Sulfur carrier protein ThiS adenylyltransferase (thiF), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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