Mouse anti-Human TCF8 Monoclonal Antibody | anti-TCF8 antibody

Mouse monoclonal antibody Anti-Human TCF8

Cat. Num. AAA120270

• Gene Names: ZEB1; BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
• Reactivity: Human
• Applications: Dot Blot
• Synonyms: TCF8; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human TCF8; Homo sapiens zinc finger E-box binding homeobox 1 (ZEB1); ZEB1; BZP; ZEB; AREB6; ZFHEP; NIL-2A; ZFHX1A; NIL-2-A; anti-TCF8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 416A7H10
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 5998

• Applicable Applications for anti-TCF8 antibody: Dot Blot (DB)
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-TCF8 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Related Product Information for anti-TCF8 antibody

TCF8 encodes a human zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991).[supplied by OMIM] [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 291575186
• NCBI GeneID: 6935
• NCBI Accession #: NP_110378.3
• NCBI GenBank Nucleotide #: NM_030751
• Molecular Weight: 122,084 Da
• NCBI Official Full Name: Homo sapiens zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: zinc finger E-box binding homeobox 1
• NCBI Official Symbol: ZEB1
• NCBI Official Synonym Symbols: BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
• NCBI Protein Information: zinc finger E-box-binding homeobox 1; delta-crystallin enhancer binding factor 1; negative regulator of IL2; posterior polymorphous corneal dystrophy 3; transcription factor 8 (represses interleukin 2 expression); zinc finger homeodomain enhancer-binding protein
• UniProt Protein Name: Zinc finger E-box-binding homeobox 1
• UniProt Gene Name: ZEB1
• UniProt Entry Name: ZEB1_HUMAN

NCBI Description

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]

Uniprot Description

TCF8: Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. Interacts (via N-terminus) with SMARCA4/BRG1. Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas. Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription, coactivator/corepressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p11.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; transcription coactivator activity; double-stranded DNA binding; chromatin binding; transcription factor binding; transcription corepressor activity; transcription factor activity

Biological Process: response to nutrient levels; transcription, DNA-dependent; regulation of T cell differentiation in the thymus; semicircular canal morphogenesis; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; pattern specification process; embryonic skeletal morphogenesis; regulation of mesenchymal cell proliferation; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; cartilage development; regulation of smooth muscle cell differentiation; forebrain development; positive regulation of transcription from RNA polymerase II promoter; immune response; response to activity; embryonic camera-type eye morphogenesis; positive regulation of neuron differentiation; negative regulation of transcription, DNA-dependent

Disease: Corneal Dystrophy, Posterior Polymorphous, 3; Corneal Dystrophy, Fuchs Endothelial, 6