WD-40 repeat-containing protein MSI1 (MSI1) Recombinant Protein | MSI1 recombinant protein
Recombinant Arabidopsis thaliana WD-40 repeat-containing protein MSI1 (MSI1)
NCBI and Uniprot Product Information
NCBI Description
Encodes a WD-40 repeat containing protein that functions in chromatin assembly as part of the CAF1 and FIE complex. Mutants exhibit parthenogenetic development that includes proliferation of unfertilized endosperm and embryos. In heterozygous plants 50% of embryos abort. Of the aborted embryos the early aborted class are homozygous and the later aborting lass are heterozygotes in which the defective allele is maternally transmitted. Other phenotypes include defects in ovule morphogenesis and organ initiation,as well as increased levels of heterochromatic DNA. MSI1 is needed for the transition to flowering. In Arabidopsis, the three CAF-1 subunits are encoded by FAS1, FAS2 and, most likely, MSI1, respectively. Mutations in FAS1 or FAS2 lead to increased frequency of homologous recombination and T-DNA integration in Arabidopsis. In the ovule, the MSI1 transcripts are accumulated at their highest level before fertilization and gradually decrease after fertilization. MSI is biallelically expressed, the paternall allele is expressed in the endosperm and embryo and is not imprinted. MSI1 forms a complex with RBR1 that is required for activation of the imprinted genes FIS2 and FWA. This activation is mediated by MSI1/RBR1 mediated repression of MET1.
Uniprot Description
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair, and the fertilization independent seed (FIS) complex, a polycomb group protein complex which is required to maintain the transcriptionally repressive state of homeotic genes throughout development. Required for several aspects of plant development including normal leaf expansion, correct development of flowers, normal endosperm development, repression of parthenogenetic seed development and repression of floral homeotic genes in leaf tissue. Also required for the recruitment of chromosomal DNA into heterochromatic chromocenters. Also involved in regulation of imprinted genes. Acts together with RBR1 to repress the expression of MET1 during female gametogenesis. This in turn activates expression of the imprinted genes FIS2 and FWA.
Research Articles on MSI1
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Product Notes
The MSI1 msi1 (Catalog #AAA1185653) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-424, Full length protein. The amino acid sequence is listed below: MGKDEEEMRG EIEERLINEE YKIWKKNTPF LYDLVITHAL EWPSLTVEWL PDREEPSGKD YSVQKMILGT HTSESEPNYL MLAQVQLPLD DTESEARQYD DDRSEFGGFG CATGKVQIIQ QINHDGEVNR ARYMPQNPFI IATKTVNAEV YVFDYSKHPS KPPLDGACNP DLKLRGHSSE GYGLSWSKFK QGHLLSGSDD AQICLWDINA TPKNKSLDAQ QIFKAHEGVV EDVAWHLRHE YLFGSVGDDQ YLLIWDLRSP SASKPVQSVV AHSMEVNCLA FNPFNEWVVA TGSTDKTVKL FDLRKLSTAL HTFDSHKEEV FQVGWNPKNE TILASCCLGR RLMVWDLSRI DEEQTVEDAE DGPPELLFIH GGHTSKISDF SWNPCEDWVI SSVAEDNILQ IWQMAENIYH DEDDAPGEEP SKAS. It is sometimes possible for the material contained within the vial of "WD-40 repeat-containing protein MSI1 (MSI1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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