L-threonine dehydratase biosynthetic IlvA (ilvA) Recombinant Protein | ilvA recombinant protein

Recombinant Escherichia coli L-threonine dehydratase biosynthetic IlvA (ilvA)

Cat. Num. AAA1142762

• Gene Names: ilvA; ECK3764; JW3745
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: L-threonine dehydratase biosynthetic IlvA (ilvA); Recombinant Escherichia coli L-threonine dehydratase biosynthetic IlvA (ilvA); ilvA recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-514, full length protein
• Sequence: MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRLDNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLGVKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHPMVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSACLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKDLFEDVRAVAEPSGALALAGMKKYIALHNIRGERLAHILSGANVNFHGLRYVSERCELGEQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEERKEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRFLNTLGTYWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAFRFFLAG
• Sequence Length: 514

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 16131630
• NCBI GeneID: 948287
• NCBI Accession #: NP_418220.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P04968; Q2M881
• Molecular Weight: 56,195 Da
• NCBI Official Full Name: l-threonine dehydratase, biosynthetic; also known as threonine deaminase
• NCBI Official Symbol: ilvA
• NCBI Official Synonym Symbols: ECK3764; JW3745
• NCBI Protein Information: l-threonine dehydratase, biosynthetic; also known as threonine deaminase
• UniProt Protein Name: L-threonine dehydratase biosynthetic IlvA
• Protein Family: L-threonine dehydratase biosynthetic
• UniProt Gene Name: ilvA

NCBI Description

N-terminal sequence of cloned IlvA shows fMet is clipped, but natural RBS was not used (Eisenstein, 1991) so it is not Verified, but the ATG start is reliable based on comparisons to Salmonella. [More information is available at EcoGene: EG10493]. Threonine deaminase (IlvA) carries out the first step in the synthesis of isoleucine. [More information is available at EcoCyc: EG10493].

Uniprot Description

Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA.