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HIT-like protein hinT (hinT) Recombinant Protein | hinT recombinant protein

Recombinant Escherichia coli HIT-like protein hinT (hinT)

Gene Names
hinT; ECK1089; JW1089; ycfF
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
HIT-like protein hinT (hinT); Recombinant Escherichia coli HIT-like protein hinT (hinT); hinT recombinant protein
Ordering
For Research Use Only!
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-119, full length protein
Sequence
MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIPNILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHMHLLGGRPLGPMLAHKGL
Sequence Length
119
Species
Escherichia coli (strain K12)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,241 Da
NCBI Official Full Name
purine nucleoside phosphoramidase, dadA activator protein
NCBI Official Symbol
hinT
NCBI Official Synonym Symbols
ECK1089; JW1089; ycfF
NCBI Protein Information
purine nucleoside phosphoramidase, dadA activator protein
UniProt Protein Name
Purine nucleoside phosphoramidase
UniProt Gene Name
hinT
UniProt Synonym Gene Names
HIT protein

NCBI Description

HinT hydrolyzes lysyl-AMP, an activity that may be involved in the regulation of translation or the scavanging of aminoacyl-adenylates (Chou, 2006). hinT is essential for growth under high salt conditions (Chou, 2005). [More information is available at EcoGene: EG12172]. HinT is a purine nucleoside phosphoramidase with a preference for purine nucleoside phosphoramidates. [More information is available at EcoCyc: EG12172].

Uniprot Description

Hydrolyzes purine nucleotide phosphoramidates, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate), guanosine 5'monophosphomorpholidate (GMP-morpholidate) and tryptamine 5'guanosine monophosphate (TpGd) (PubMed:15703176, PubMed:20934431). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine--tRNA ligase, and lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) (PubMed:15703176). Is essential for the activity of the enzyme D-alanine dehydrogenase (DadA) and is required for E.coli to grow on D-alanine as a sole carbon source (PubMed:21754980). Is also required for growth at high salt concentrations (PubMed:15703176).

Research Articles on hinT

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Product Notes

The hinT hint (Catalog #AAA1130558) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-119, full length protein. The amino acid sequence is listed below: MAEETIFSKI IRREIPSDIV YQDDLVTAFR DISPQAPTHI LIIPNILIPT VNDVSAEHEQ ALGRMITVAA KIAEQEGIAE DGYRLIMNTN RHGGQEVYHI HMHLLGGRPL GPMLAHKGL. It is sometimes possible for the material contained within the vial of "HIT-like protein hinT (hinT), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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