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Ribosomal RNA large subunit methyltransferase N (rlmN) Recombinant Protein | rlmN recombinant protein

Recombinant Shewanella sp. Ribosomal RNA large subunit methyltransferase N (rlmN)

Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Ribosomal RNA large subunit methyltransferase N (rlmN); Recombinant Shewanella sp. Ribosomal RNA large subunit methyltransferase N (rlmN); rlmN recombinant protein
Ordering
For Research Use Only!
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-373, Full length protein
Sequence
MSEKKINLLDLDRKAMRALFADLGEKPFRADQLMKWIYHFGVSDFEEMTNINKVLRQKLAARCEIVAPEISSFQKSTDGTIKFAIHVGEGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTVSEIVGQIWRVSHFLGFAKDTGDRPITNVVMMGMGEPLLNLANVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDALDVALAVSIHAPNDELRDILVPVNKKYPLQEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTEQAHELAKLMKDTPCKVNLIPFNPYPGSPYGRSSNSRIDRFSKVLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKRMQENQISVTMN
Sequence Length
373
Species
Shewanella sp. (strain W3-18-1)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41,668 Da
NCBI Official Full Name
MULTISPECIES: bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN
UniProt Protein Name
Dual-specificity RNA methyltransferase RlmN
UniProt Gene Name
rlmN

Uniprot Description

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.

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Product Notes

The rlmN rlmn (Catalog #AAA1100153) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-373, Full length protein. The amino acid sequence is listed below: MSEKKINLLD LDRKAMRALF ADLGEKPFRA DQLMKWIYHF GVSDFEEMTN INKVLRQKLA ARCEIVAPEI SSFQKSTDGT IKFAIHVGEG QEVETVYIPE DDRATLCVSS QVGCALECTF CSTAQQGFNR NLTVSEIVGQ IWRVSHFLGF AKDTGDRPIT NVVMMGMGEP LLNLANVIPA MDIMLDDFGF SLSKRRVTLS TSGVVPALDK LGDALDVALA VSIHAPNDEL RDILVPVNKK YPLQEFLAGI RRYIAKSNAN RGRVTVEYVM LDHINDSTEQ AHELAKLMKD TPCKVNLIPF NPYPGSPYGR SSNSRIDRFS KVLMEYGLTV IVRKTRGDDI DAACGQLAGD IRDRTKRLAK KRMQENQISV TMN. It is sometimes possible for the material contained within the vial of "Ribosomal RNA large subunit methyltransferase N (rlmN), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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