Cyclic nucleotide-gated ion channel 2 (CNGC2) Recombinant Protein | DND1 recombinant protein

Recombinant Arabidopsis thaliana Cyclic nucleotide-gated ion channel 2 (CNGC2)

• Gene Names: DND1; ATCNGC2; CNGC2; CYCLIC NUCLEOTIDE GATED CHANNEL 2; CYCLIC NUCLEOTIDE-GATED CHANNEL 2; DEFENSE NO DEATH 1; DND1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Cyclic nucleotide-gated ion channel 2 (CNGC2); Recombinant Arabidopsis thaliana Cyclic nucleotide-gated ion channel 2 (CNGC2); Recombinant Cyclic nucleotide-gated ion channel 2 (CNGC2); Cyclic nucleotide-gated ion channel 2; AtCNGC2; Cyclic nucleotide- and calmodulin-regulated ion channel 2 Protein DEFENSE NO DEATH 1; DND1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-726
• Sequence: MPSHPNFIFRWIGLFSDKFRRQTTGIDENSNLQINGGDSSSSGSDETPVLSSVECYACTQVGVPAFHSTSCDQAHAPEWRASAGSSLVPIQEGSVPNPARTRFRRLKGPFGEVLDPRSKRVQRWNRALLLARGMALAVDPLFFYALSIGRTTGPACLYMDGAFAAVVTVLRTCLDAVHLWHVWLQFRLAYVSRESLVVGCGKLVWDPRAIASHYARSLTGFWFDVIVILPVPQAVFWLVVPKLIREEKVKLIMTILLLIFLFQFLPKIYHCICLMRRMQKVTGYIFGTIWWGFALNLIAYFIASHVAGGCWYVLAIQRVASCIRQQCMRTGNCNLSLACKEEVCYQFVSPTSTVGYPCLSGNLTSVVNKPMCLDSNGPFRYGIYRWALPVISSNSLAVKILYPIFWGLMTLSTFANDLEPTSNWLEVIFSIVMVLSGLLLFTLLIGNIQVFLHAVMAKKRKMQIRCRDMEWWMKRRQLPSRLRQRVRRFERQRWNALGGEDELELIHDLPPGLRRDIKRYLCFDLINKVPLFRGMDDLILDNICDRAKPRVFSKDEKIIREGDPVQRMIFIMRGRVKRIQSLSKGVLATSTLEPGGYLGDELLSWCLRRPFLDRLPPSSATFVCLENIEAFSLGSEDLRYITDHFRYKFANERLKRTARYYSSNWRTWAAVNIQMAWRRRRKRTRGENIGGSMSPVSENSIEGNSERRLLQYAAMFMSIRPHDHLE
• Sequence Length: 726

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

NCBI and Uniprot Product Information

• NCBI GI #: 15242291
• NCBI GeneID: 831393
• NCBI Accession #: NP_197045.1
• NCBI GenBank Nucleotide #: NM_121545.4
• UniProt Accession #: O65718
• Molecular Weight: 83,241 Da
• NCBI Official Full Name: cyclic nucleotide-gated ion channel 2
• NCBI Official Symbol: DND1
• NCBI Official Synonym Symbols: ATCNGC2; CNGC2; CYCLIC NUCLEOTIDE GATED CHANNEL 2; CYCLIC NUCLEOTIDE-GATED CHANNEL 2; DEFENSE NO DEATH 1; DND1
• NCBI Protein Information: cyclic nucleotide-gated ion channel 2
• UniProt Protein Name: Cyclic nucleotide-gated ion channel 2
• Protein Family: Dead end protein
• UniProt Gene Name: CNGC2
• UniProt Synonym Gene Names: DND1; AtCNGC2
• UniProt Entry Name: CNGC2_ARATH

Uniprot Description

Function: Acts as cyclic nucleotide-gated ion channel. Permeable to potassium and calcium in a cyclic nucleotide-dependent fashion (cAMP or cGMP). Could also transport lithium, cesium and rubium and displays a strong selectivity against sodium. Seems to directly participate in pathogen-induced calcium influx. May function in homeostasis, re-establishing ionic balance after defense action and/or other stimuli. Could mediate the initiation of the developmentally regulated cell death programs. Ref.3 Ref.8

Subunit structure: Homotetramer or heterotetramer

Potential. Binds calmodulin-1/4 with a higher affinity than calmodulin-2/3/5.

Subcellular location: Cell membrane; Multi-pass membrane protein

Potential.

Tissue specificity: Expressed in the whole plant but only weakly in roots. Strongly expressed in the expanded cotyledons of 14-day-old seedlings and detected later in leaves after the transition to flowering. Also detected in flowers during organ senescence and in the dehiscence zone of siliques. Ref.8

Induction: Up-regulated by light. Transiently induced during leaf and culture senescence. Ref.8

Domain: The binding of calmodulin to the C-terminus might interfere with cyclic nucleotide binding and thus channel activation

By similarity. Ref.7

Miscellaneous: Loss-of-function mutations cngc2-1 (dnd1-1) or cncg2-2 results in the loss of the hypersensitive response and leads to a broad spectrum disease resistance. These mutations lead to a specific and dramatic calcium hypersensitivity that results in severe reductions in plant size and seed yield.

Sequence similarities: Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. [View classification]Contains 1 cyclic nucleotide-binding domain.Contains 1 IQ domain.