Mouse Fibronectin Type III Domain Containing Protein 5 ELISA Kit | FNDC5 elisa kit

Mouse Fibronectin Type III Domain Containing Protein 5 ELISA Kit

Cat. Num. AAA107547

• Gene Names: FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
• Reactivity: Mouse
• Synonyms: Fibronectin Type III Domain Containing Protein 5; Mouse Fibronectin Type III Domain Containing Protein 5 ELISA Kit; FNDC5 elisa kit

• Reactivity: Mouse
• Specificity: No significant cross-reactivity or interference between Mouse FNDC5 and analogues was observed.
• Sequence Length: 2386

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 31.2 pg/ml - 1000 pg/ml.
• Sensitivity: 5.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated FNDC5 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse FNDC5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FNDC5 and FNDC5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FNDC5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for FNDC5 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated FNDC5 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse FNDC5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FNDC5 and FNDC5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FNDC5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 300669710
• NCBI GeneID: 2335
• UniProt Accession #: P02751; O95609; O95610; Q14312; Q14325; Q14326; Q17RV7; Q564H7; Q585T2; B7ZLF0; E9PE77; E9PG29
• Molecular Weight: 262,625 Da
• NCBI Official Full Name: Fibronectin
• NCBI Official Synonym Full Names: fibronectin 1
• NCBI Official Symbol: FN1
• NCBI Official Synonym Symbols: FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
• NCBI Protein Information: fibronectin; cold-insoluble globulin; migration-stimulating factor
• UniProt Protein Name: Fibronectin
• Protein Family: Fibronectin type III domain-containing protein
• UniProt Gene Name: FN1
• UniProt Synonym Gene Names: FN; FN; CIG
• UniProt Entry Name: FINC_HUMAN

NCBI Description

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: extracellular matrix; extracellular space; apical plasma membrane; fibrinogen complex; extracellular region; ER-Golgi intermediate compartment; basal lamina

Molecular Function: collagen binding; integrin binding; heparin binding; protein binding; protease activator activity; protease binding

Biological Process: integrin activation; platelet activation; extracellular matrix organization and biogenesis; positive regulation of axon extension; extracellular matrix disassembly; regulation of cell shape; platelet degranulation; cell-substrate junction assembly; calcium-independent cell-matrix adhesion; response to wounding; acute-phase response; peptide cross-linking; angiogenesis; blood coagulation; cell adhesion; leukocyte migration

Disease: Glomerulopathy With Fibronectin Deposits 2; Plasma Fibronectin Deficiency