Sensor histidine kinase DcuS (dcuS) Recombinant Protein | dcuS recombinant protein

Recombinant Escherichia coli Sensor histidine kinase DcuS (dcuS)

• Gene Names: dcuS; ECK4118; JW4086; yjdH
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Sensor histidine kinase DcuS (dcuS); Recombinant Escherichia coli Sensor histidine kinase DcuS (dcuS); dcuS recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-543
• Sequence: MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQISDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTDMQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQIGVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEISTLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQVVDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRLDGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKIKSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEALGPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVKQQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR
• Sequence Length: 543

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Related Product Information for dcuS recombinant protein

dcuS; yjdH

NCBI and Uniprot Product Information

• NCBI GI #: 16131951
• NCBI GeneID: 948639
• NCBI Accession #: NP_418549.1
• NCBI GenBank Nucleotide #: NC_000913.2
• UniProt Accession #: P0AEC8; P39272; P76795; Q2M6H9
• Molecular Weight: 60,551 Da
• NCBI Official Full Name: sensory histidine kinase in two-component regulatory system with DcuR, regulator of anaerobic fumarate respiration
• NCBI Official Symbol: dcuS
• NCBI Official Synonym Symbols: ECK4118; JW4086; yjdH
• NCBI Protein Information: sensory histidine kinase in two-component regulatory system with DcuR, regulator of anaerobic fumarate respiration
• UniProt Protein Name: Sensor histidine kinase DcuS
• Protein Family: Sensor histidine kinase
• UniProt Gene Name: dcuS
• UniProt Synonym Gene Names: yjdH
• UniProt Entry Name: DCUS_ECOLI

NCBI Description

dcuSR operon is repressed by nitrate via the NarXL system. [More information is available at EcoGene: EG12465]. DcuS acts as a C4-dicarboxylate-sensing histidine kinase . [More information is available at EcoCyc: G7827].

Uniprot Description

Function: Member of the two-component regulatory system DcuR/DcuS. Involved in the C4-dicarboxylate-stimulated regulation of the genes encoding the anaerobic fumarate respiratory system (frdABCD; nuoAN; dcuB; dcuC; sdhCDAB; etc.). Weakly regulates the aerobic C4-dicarboxylate transporter dctA. Activates DcuR by phosphorylation.

Catalytic activity: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Enzyme regulation: Autophosphorylation is stimulated by the presence of C4-dicarboxylates such as fumarate, succinate, malate, and tartrate.

Subunit structure: Homodimer

Probable. Ref.9 Ref.10

Subcellular location: Cell inner membrane; Multi-pass membrane protein.

Domain: The periplasmic domain is involved in C4-dicarboxylate binding and sensing. The structural disorder in the cytoplasmic PAS domain has an important role in signal transduction to the kinase domain and may be the decisive structural feature that characterizes the activated kinase. Ref.8 Ref.9 Ref.10

Post-translational modification: Autophosphorylated. The phosphoryl group is rapidly transferred to DcuR.

Miscellaneous: The region encompassing approximately residues 42 to 181 has been shown to be periplasmic, however exactly which residues are periplasmic is not clear.

Sequence similarities: Contains 1 histidine kinase domain.Contains 1 PAS (PER-ARNT-SIM) domain.