Sensor protein PhoQ (phoQ) Recombinant Protein | phoQ recombinant protein

Recombinant Escherichia coli Sensor protein PhoQ (phoQ)

• Gene Names: phoQ; ECK1115; JW1115
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Sensor protein PhoQ (phoQ); Recombinant Escherichia coli Sensor protein PhoQ (phoQ); Recombinant Sensor protein PhoQ (phoQ); Sensor protein PhoQ EC= 2.7.13.3; phoQ recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-486
• Sequence: MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSFDKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDVPWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVAVNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSLRPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLTHSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLSRELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACKYCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAREITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE
• Sequence Length: 486

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

NCBI and Uniprot Product Information

• NCBI GI #: 16129092
• NCBI GeneID: 946326
• NCBI Accession #: NP_415647.1
• NCBI GenBank Nucleotide #: NC_000913.2
• UniProt Accession #: P23837
• Molecular Weight: 55,300 Da
• NCBI Official Full Name: sensory histidine kinase in two-component regulatory system with PhoP
• NCBI Official Symbol: phoQ
• NCBI Official Synonym Symbols: ECK1115; JW1115
• NCBI Protein Information: sensory histidine kinase in two-component regulatory system with PhoP
• UniProt Protein Name: Sensor protein PhoQ
• Protein Family: Sensor protein
• UniProt Gene Name: phoQ
• UniProt Entry Name: PHOQ_ECOLI

NCBI Description

PhoPQ has been primarily characterized in Salmonella, with significant differences from Escherichia coli. [More information is available at EcoGene: EG10732]. The subunit structure in vivo is not known. [More information is available at EcoCyc: EG10732].

Uniprot Description

Function: Member of the two-component regulatory system PhoQ/PhoP involved in adaptation to low Mg2+ environments and the control of acid resistance genes. In presence of low periplasmic Mg2+ concentrations, PhoQ functions as a membrane-associated protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to PhoP, which results in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In presence of high periplasmic Mg2+ concentrations, acts as a protein phosphatase that dephosphorylates phospho-PhoP, which results in the repression of PhoP-activated genes and may lead to expression of some PhoP-repressed genes

By similarity. Mediates magnesium influx to the cytosol by activation of mgtA. Promotes expression of the two-component regulatory system rstA/rstB and transcription of the hemL, mgrB, nagA, slyB, vboR and yrbL genes. Ref.7

Catalytic activity: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Enzyme regulation: Acetyl-CoA acts as a non-competitive inhibitor of the PhoQ autokinase activity. Ref.9

Subunit structure: Homodimer

Probable. Ref.18

Subcellular location: Cell inner membrane; Multi-pass membrane protein Ref.15.

Induction: PhoQ/phoP operon is positively autoregulated by both PhoP and PhoQ in a Mg2+-dependent manner. Ref.7 Ref.9 Ref.10 Ref.14

Miscellaneous: There is a close linkage between the PhoQ/PhoP and Rcs signaling systems, and both signaling systems respond to certain external divalent cations (zinc and magnesium).Two-component regulatory system EvgA/EvgS interacts with PhoQ/PhoP via signal transduction mediated by phospho-EvgA.

Sequence similarities: Contains 1 HAMP domain.Contains 1 histidine kinase domain.

Biophysicochemical propertiesKinetic parameters:KM=20.93 µM for ATP (at 22 degrees Celsius and pH 8, in the presence of 50 mM KCl and 1 mM MgCl2) Ref.9 Ref.17KM=55 µM for ATP (at 22 degrees Celsius and pH 8, in the presence of 25 mM KCl and 0.4 mM MgCl2)