Rat Integrin Alpha M ELISA Kit | ITGalphaM elisa kit

Rat Integrin Alpha M ELISA Kit

Cat. Num. AAA100015

• Gene Names: ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
• Reactivity: Rat
• Synonyms: Integrin Alpha M; Rat Integrin Alpha M ELISA Kit; ITGalphaM elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat ITGalphaM and analogues was observed.
• Sequence Length: 1153

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 3.12 ng/ml - 100 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated ITGalphaM concentrations in Rat serum, plasma and other body fluids. Using Purified Rat ITGalphaM antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ITGalphaM and ITGalphaM antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ITGalphaM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for ITGalphaM elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated ITGalphaM concentrations in Rat serum, plasma and other body fluids. Using Purified Rat ITGalphaM antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ITGalphaM and ITGalphaM antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ITGalphaM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 224831239
• NCBI GeneID: 3684
• NCBI Accession #: NP_001139280.1
• NCBI GenBank Nucleotide #: NM_001145808.1
• UniProt Accession #: P11215; Q4VAK0; Q4VAK1; Q4VAK2
• Molecular Weight: 127,179 Da
• NCBI Official Full Name: integrin alpha-M isoform 1
• NCBI Official Synonym Full Names: integrin, alpha M (complement component 3 receptor 3 subunit)
• NCBI Official Symbol: ITGAM
• NCBI Official Synonym Symbols: CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
• NCBI Protein Information: integrin alpha-M; CR-3 alpha chain; antigen CD11b (p170); leukocyte adhesion receptor MO1; CD11 antigen-like family member B; macrophage antigen alpha polypeptide; cell surface glycoprotein MAC-1 subunit alpha; neutrophil adherence receptor alpha-M subunit
• UniProt Protein Name: Integrin alpha-M
• UniProt Gene Name: ITGAM
• UniProt Synonym Gene Names: CD11B; CR3A
• UniProt Entry Name: ITAM_HUMAN

NCBI Description

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

Function: Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

Subunit structure: Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Interacts with JAM3. Ref.10 Ref.11

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Predominantly expressed in monocytes and granulocytes.

Domain: The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

Involvement in disease: Systemic lupus erythematosus 6 (SLEB6) [MIM:609939]: A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.Note: Disease susceptibility may be associated with variations affecting the gene represented in this entry.

Sequence similarities: Belongs to the integrin alpha chain family.Contains 7 FG-GAP repeats.Contains 1 VWFA domain.