Fish 5-Nucleotidase ELISA Kit | 5NT elisa kit

Fish 5-Nucleotidase ELISA Kit

• Gene Names: Nt5e; Nt5; CD73
• Reactivity: Fish
• Synonyms: 5-Nucleotidase; Fish 5-Nucleotidase ELISA Kit; 5NT elisa kit

• Reactivity: Fish
• Specificity: No significant cross-reactivity or interference between Fish 5NT and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 3.12U/L-100U/L.
• Sensitivity: 1.0U/L.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated 5NT concentrations in Fish serum, plasma and other body fluids. Using Purified Fish 5NT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add 5NT and 5NT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 5NT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for 5NT elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated 5NT concentrations in Fish serum, plasma and other body fluids. Using Purified Fish 5NT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add 5NT and 5NT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 5NT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148747417
• NCBI GeneID: 58813
• NCBI Accession #: NP_067587.2
• NCBI GenBank Nucleotide #: NM_021576.2
• UniProt Accession #: P21588
• Molecular Weight: 63,969 Da
• NCBI Official Full Name: 5'-nucleotidase
• NCBI Official Synonym Full Names: 5' nucleotidase, ecto
• NCBI Official Symbol: Nt5e
• NCBI Official Synonym Symbols: Nt5; CD73
• NCBI Protein Information: 5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• UniProt Gene Name: Nt5e
• UniProt Synonym Gene Names: Nt5; Nte; 5'-NT
• UniProt Entry Name: 5NTD_RAT

NCBI Description

may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]

Uniprot Description

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Nucleotide Metabolism - purine; Membrane protein, integral; EC 3.1.3.5; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Nucleotide Metabolism - pyrimidine

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane; integral to membrane

Molecular Function: 5'-nucleotidase activity; ferrous iron binding; metal ion binding; nucleotide binding

Biological Process: dephosphorylation; negative regulation of inflammatory response; adenosine biosynthetic process; purine nucleotide biosynthetic process; brain development; positive regulation of lipid biosynthetic process; adenosine metabolic process; response to aluminum ion; AMP catabolic process