DNA-directed RNA polymerase (1) Recombinant Protein | 1 recombinant protein

Recombinant Enterobacteria phage T7 DNA-directed RNA polymerase (1), partial

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: DNA-directed RNA polymerase (1); Recombinant Enterobacteria phage T7 DNA-directed RNA polymerase (1); partial; 1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 883

• Species: Enterobacteria phage T7 (Bacteriophage T7)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 9627432
• NCBI GeneID: 1261050
• NCBI Accession #: NP_041960.1
• NCBI GenBank Nucleotide #: NC_001604.1
• UniProt Accession #: P00573; Q38543
• Molecular Weight: 98,855 Da
• NCBI Official Full Name: T7 RNA polymerase
• UniProt Protein Name: T7 RNA polymerase
• UniProt Gene Name: 1

Uniprot Description

Highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. Recognizes a specific promoter sequence and enters first into an "abortive phase" where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. Unwinds the double-stranded DNA to expose the coding strand for templating. Participates in the initiation of viral DNA replication presumably by making primers accessible to the DNA polymerase, thus facilitating the DNA opening. Plays also a role in viral DNA packaging, probably by pausing the transcription at the right end of concatemer junction to allow packaging complex recruitment and beginning of the packaging process.