Mouse Zinc finger protein 271 ELISA Kit | ZNF271 elisa kit
Mouse Zinc finger protein 271 (ZNF271) ELISA Kit
Gene Names
Zfp35; Zfp-35; Znf271; mszf13; mszf28; mszf68; mszf20-1
Reactivity
Mouse
Synonyms
Zinc finger protein 271; Mouse Zinc finger protein 271 (ZNF271) ELISA Kit; ZNF271 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse ZNF271. No significant cross-reactivity or interference between Mouse ZNF271 and analogues was observed.
Sequence Length
655
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ZNF271 elisa kit
Epstein-Barr virus (EBV) infects the majority of humans in adolescence, establishes latency in a small subset of B lymphocytes, and transforms cells through the coordinate expression of a selected repertoire of EBV-encoded latency genes. The deduced 423-amino acid protein contains 5 putative zinc-binding, Kruppel-type finger domains in its C-terminal portion, each separated by 7 amino acids. Expression in tonsillar B cells was low but increased transiently after B-cell receptor stimulation and mitogenic activation. After EBV infection, however, levels continued to rise, approximating the kinetics of viral latency genes. Treatment of tonsillar B cells with antisense to ZNFEB inhibited EBV-induced growth transformation and ZNFEB expression, similar to the effects of antisense to viral EBNA1 or EBNA2 or cellular LCK. ZNFEB is essential in the EBV-mediated transformation process.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZNF271 in samples. An antibody specific for ZNF271 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZNF271 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZNF271 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZNF271 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZNF271 in samples. An antibody specific for ZNF271 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZNF271 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZNF271 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZNF271 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
67,036 Da
NCBI Official Full Name
zinc finger protein 271
NCBI Official Synonym Full Names
zinc finger protein 35
NCBI Official Symbol
Zfp35
NCBI Official Synonym Symbols
Zfp-35; Znf271; mszf13; mszf28; mszf68; mszf20-1
NCBI Protein Information
zinc finger protein 271
UniProt Protein Name
Zinc finger protein 271
Protein Family
UniProt Gene Name
Znf271
UniProt Synonym Gene Names
Zfp-35; Zfp35; Zfp-35
Uniprot Description
ZNF271: May be involved in transcriptional regulation. Plays a role in the EBV-mediated transformation process. Belongs to the krueppel C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 18|18 A2
Cellular Component: nucleus
Molecular Function: DNA binding; DNA binding transcription factor activity; metal ion binding; nucleic acid binding
Biological Process: cell differentiation; inflammatory response to antigenic stimulus; multicellular organism development; negative regulation of T-helper 2 cell differentiation; negative regulation of T-helper 2 type immune response; regulation of transcription, DNA-templated; spermatogenesis; transcription, DNA-dependent
Research Articles on ZNF271
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Product Notes
The Mouse ZNF271 znf271 (Catalog #AAA282469) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282469 ELISA Kit recognizes Mouse ZNF271. It is sometimes possible for the material contained within the vial of "Zinc finger protein 271, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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