Mouse Zinc finger protein 36, C3H1 type-like 1 ELISA Kit | ZFP36L1 elisa kit

Mouse Zinc finger protein 36, C3H1 type-like 1 (ZFP36L1) ELISA Kit

Cat. Num. AAA283094

• Gene Names: Zfp36l1; Brf1; ERF1; cMG1; Berg36; TIS11b; AW742437; AW743212; D530020L18Rik
• Reactivity: Mouse
• Synonyms: Zinc finger protein 36; C3H1 type-like 1; Mouse Zinc finger protein 36; C3H1 type-like 1 (ZFP36L1) ELISA Kit; ZFP36L1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse ZFP36L1. No significant cross-reactivity or interference between Mouse ZFP36L1 and analogues was observed.
• Sequence Length: 338

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ZFP36L1 elisa kit

ZFP36L1 is a member of the TIS11 family of early response genes. The gene is well conserved across species and has a promoter that contains motifs seen in other early-response genes. The encoded protein contains a distinguishing putative zinc finger domain with a repeating cys-his motif. The deduced 338-amino acid BERG36 protein could be induced by treatment with calcium ionophore, and the induction could be blocked by treatment with interleukin-4 (IL4) but not by CD40 ligation. Treatment of the Epstein-Barr virus-negative human Burkitt lymphoma cell line Ramos, which phenotypically resembles germinal center B cells, with BERG36-antisense or with IL4 or CD40 ligation protected the cells from ionophore-induced apoptosis.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZFP36L1 in samples. An antibody specific for ZFP36L1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZFP36L1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZFP36L1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZFP36L1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6680808
• NCBI GeneID: 12192
• NCBI Accession #: NP_031590.1
• NCBI GenBank Nucleotide #: NM_007564.5
• UniProt Accession #: P23950
• Molecular Weight: 36,385 Da
• NCBI Official Full Name: mRNA decay activator protein ZFP36L1
• NCBI Official Synonym Full Names: zinc finger protein 36, C3H type-like 1
• NCBI Official Symbol: Zfp36l1
• NCBI Official Synonym Symbols: Brf1; ERF1; cMG1; Berg36; TIS11b; AW742437; AW743212; D530020L18Rik
• NCBI Protein Information: mRNA decay activator protein ZFP36L1
• UniProt Protein Name: mRNA decay activator protein ZFP36L1
• Protein Family: mRNA decay activator protein
• UniProt Gene Name: Zfp36l1
• UniProt Synonym Gene Names: ZFP36-like 1

Uniprot Description

BRF1: a zinc finger protein that plays an important role in the assembly of the RNA polymerase III initiation factor TFIIIB. Regulates mRNA levels by targeting transcripts containing AREs (AU-rich elements) into the decay pathway. Phosphorylation by Akt apparently generates 14-3-3 binding sites and inhibits BRF1 from promoting mRNA activity.Contains 2 C3H1-type zinc fingers.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 12|12 C3

Cellular Component: cytoplasm; cytosol; intracellular ribonucleoprotein complex; nucleus; P-body

Molecular Function: 14-3-3 protein binding; AU-rich element binding; DNA binding; metal ion binding; mRNA binding; protein binding; RNA binding

Biological Process: apoptosis; cell proliferation; cellular response to cAMP; cellular response to epidermal growth factor stimulus; cellular response to fibroblast growth factor stimulus; cellular response to glucocorticoid stimulus; cellular response to hypoxia; cellular response to insulin stimulus; cellular response to peptide hormone stimulus; cellular response to phorbol 13-acetate 12-myristate; cellular response to salt stress; cellular response to transforming growth factor beta stimulus; cellular response to tumor necrosis factor; embryonic organ development; ERK1 and ERK2 cascade; heart development; MAPK cascade; mesendoderm development; mRNA catabolic process; mRNA catabolic process, deadenylation-dependent decay; mRNA catabolic process, deadenylation-independent decay; mRNA processing; mRNA transport; multicellular organism development; multicellular organism growth; negative regulation of erythrocyte differentiation; negative regulation of mitotic cell cycle phase transition; neural tube development; p38MAPK cascade; phosphoinositide 3-kinase cascade; positive regulation of fat cell differentiation; positive regulation of intracellular mRNA localization; positive regulation of monocyte differentiation; positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; proepicardium development; protein kinase B signaling; regulation of B cell differentiation; regulation of gene expression; regulation of keratinocyte apoptotic process; regulation of keratinocyte differentiation; regulation of keratinocyte proliferation; regulation of mRNA 3'-end processing; regulation of mRNA stability; regulation of myoblast differentiation; regulation of stem cell proliferation; regulation of translation; response to wounding; T cell differentiation in thymus; transport; vasculogenesis