Mouse Zinc finger protein 106 homolog ELISA Kit | ZFP106 elisa kit
Mouse Zinc finger protein 106 homolog (ZFP106) ELISA Kit
Reactivity
Mouse
Synonyms
Zinc finger protein 106 homolog; Mouse Zinc finger protein 106 homolog (ZFP106) ELISA Kit; ZFP106 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse ZFP106. No significant cross-reactivity or interference between Mouse ZFP106 and analogues was observed.
Sequence Length
1883
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ZFP106 elisa kit
ZFP106 a protein with six WD40 domains and a zinc-finger domain. May be involved in growth related metabolism. A region of ZFP106 is identical to a 600-amino acid sequence implicated in the insulin receptor signaling pathway. Regulated by NRF-1 and myogenin, and expressed in brown fat, developing brain, and striated muscle.
The WD40 repeats containing zinc finger protein 106 (ZFP106)is a conserved mammalian protein of unknown function. However, its cDNA shares an extended region of identity with Sh3bp3 cDNA encoding a protein implicated in the insulin signaling pathway. In situ hybridization of mouse embryos confirmed predominant expression of Zfp106 in tissues with high developmental expression of either NRF-1 or myogenin.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZFP106 in samples. An antibody specific for ZFP106 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZFP106 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZFP106 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZFP106 bound in the initial step. The color development is stopped and the intensity of the color is measured.
The WD40 repeats containing zinc finger protein 106 (ZFP106)is a conserved mammalian protein of unknown function. However, its cDNA shares an extended region of identity with Sh3bp3 cDNA encoding a protein implicated in the insulin signaling pathway. In situ hybridization of mouse embryos confirmed predominant expression of Zfp106 in tissues with high developmental expression of either NRF-1 or myogenin.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZFP106 in samples. An antibody specific for ZFP106 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZFP106 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZFP106 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZFP106 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
94,224 Da
NCBI Official Full Name
Zinc finger protein 106
UniProt Protein Name
Zinc finger protein 106
UniProt Gene Name
Znf106
UniProt Synonym Gene Names
H3a; Sh3bp3; Sirm; Zfp106; Znf474; Zfp-106
Uniprot Description
ZFP106: a protein with six WD40 domains and a zinc-finger domain. May be involved in growth related metabolism. A region of ZFP106 is identical to a 600-amino acid sequence implicated in the insulin receptor signaling pathway. Regulated by NRF-1 and myogenin, and expressed in brown fat, developing brain, and striated muscle.
Protein type: Adaptor/scaffold; C2H2-type zinc finger protein; Nucleolus
Chromosomal Location of Human Ortholog: 2 E5|2 60.37 cM
Cellular Component: cytoplasm; cytosol; membrane; nucleus
Molecular Function: metal ion binding; nucleic acid binding; opioid peptide activity; RNA binding; SH3 domain binding
Biological Process: insulin receptor signaling pathway
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Product Notes
The Mouse ZFP106 znf106 (Catalog #AAA282224) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282224 ELISA Kit recognizes Mouse ZFP106. It is sometimes possible for the material contained within the vial of "Zinc finger protein 106 homolog, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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