VTCN1 recombinant protein

VTCN1, 25-259aa, Human, His-tag, Baculovirus

Cat. Num. AAA206657

• Gene Names: VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: VTCN1; 25-259aa; Human; His-tag; Baculovirus; V-set domain-containing T-cell activation inhibitor 1 isoform 1; B7-H4; B7h.5; B7H4; B7S1; B7X; PRO1291; VTCN1 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPLIIGFGI SGRHSITVTT VASAGNIGED GILSCTFEPD IKLSDIVIQW LKEGVLGLVH EFKEGKDELS EQDEMFRGRT AVFADQVIVG NASLRLKNVQ LTDAGTYKCY IITSKGKGNA NLEYKTGAFS MPEVNVDYNA SSETLRCEAP RWFPQPTVVW ASQVDQGANF SEVSNTSFEL NSENVTMKVV SVLYNVTINN TYSCMIENDI AKATGDIKVT ESEIKRRSHL QLLNSKASHH HHHH
• Sequence Length: 282

• Applicable Applications for VTCN1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for VTCN1 recombinant protein

VTCN1, also known as v-set domain-containing T-cell activation inhibitor 1 isoform 1, is a member of the costimulatory B7 family. It has been found to be widely expressed in many kinds of tumor tissues and to play an important part in tumor progression and poor prognosis. It serves a critical role in the negative regulation of T cell-mediated antitumor immune responses. It facilitates proliferation of esophageal squamous cell carcinoma cells through promoting interleukin-6/signal transducer and activator of transcription 3 pathway activation. Recombinant human VTCN1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for VTCN1 recombinant protein

Cell Cycle

References

Zeng S., et al. (2016) Mol Biol. 50:1007-1013.; Chen X., et al. (2016) Cancer Sci. 107:944-954.

NCBI and Uniprot Product Information

• NCBI GI #: 99028881
• NCBI GeneID: 79679
• NCBI Accession #: NP_078902
• NCBI GenBank Nucleotide #: NM_024626.3
• UniProt Accession #: Q7Z7D3; Q0GN76; Q45VN0; Q5WPZ3; Q6P097; Q9H6B2
• Molecular Weight: 26.9kDa (244aa) 28-40kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: V-set domain-containing T-cell activation inhibitor 1 isoform 1
• NCBI Official Synonym Full Names: V-set domain containing T cell activation inhibitor 1
• NCBI Official Symbol: VTCN1
• NCBI Official Synonym Symbols: B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
• NCBI Protein Information: V-set domain-containing T-cell activation inhibitor 1
• UniProt Protein Name: V-set domain-containing T-cell activation inhibitor 1
• Protein Family: V-set domain containing T-cell activation inhibitor
• UniProt Gene Name: VTCN1
• UniProt Synonym Gene Names: B7-H4

NCBI Description

This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.