U2AF1 recombinant protein

Recombinant Human U2AF1

Cat. Num. AAA7613220

• Gene Names: U2AF1; RN; FP793; U2AF35; U2AFBP; RNU2AF1
• Applications: Western Blot, ELISA
• Purity: Greater than 70% by SDS-PAGE gel analyses
• Synonyms: U2AF1; Recombinant Human U2AF1; FP793; RN; RNU2AF1; U2AF35; U2AFBP; U2AF1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 70% by SDS-PAGE gel analyses
• Form/Format: Lyophilized from a 0.2um filtered solution in PBS with 5% trehalose, pH7.4
• Sequence Positions: 100-240

• Applicable Applications for U2AF1 recombinant protein: Western Blot (WB), ELISA (EIA)
• Species: Human
• Tag: N-terminal His-IF2DI Tag
• Reconstitution: Centrifuge the vial at 10,000rpm for 1 minute, reconstitute at 200ug/ml in sterile distilled water
• Preparation and Storage: Store at -20 degree C for 12 months as lyophilized; 2-8 degree C for 1 month under sterile conditions after reconstitution

NCBI and Uniprot Product Information

• NCBI GI #: 267187
• NCBI GeneID: 7307
• UniProt Accession #: Q01081; Q701P4; Q71RF1
• Molecular Weight: 240
• NCBI Official Full Name: Splicing factor U2AF 35 kDa subunit
• NCBI Official Synonym Full Names: U2 small nuclear RNA auxiliary factor 1
• NCBI Official Symbol: U2AF1
• NCBI Official Synonym Symbols: RN; FP793; U2AF35; U2AFBP; RNU2AF1
• NCBI Protein Information: splicing factor U2AF 35 kDa subunit; splicing factor U2AF 35kDa subunit; U2 small nuclear RNA auxillary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor binding protein; U2 small nuclear ribonucleoprotein auxi
• UniProt Protein Name: Splicing factor U2AF 35 kDa subunit
• Protein Family: Splicing factor
• UniProt Gene Name: U2AF1
• UniProt Synonym Gene Names: U2AF35; U2AFBP
• UniProt Entry Name: U2AF1_HUMAN

NCBI Description

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

U2AF1: Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron. Belongs to the splicing factor SR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; RNA splicing; RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleoplasm; spliceosome; Cajal body; nuclear speck

Molecular Function: RS domain binding; protein binding; metal ion binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription