Thioredoxin reductase 1 Recombinant Protein | TRXR1 recombinant protein

Recombinant Human Thioredoxin reductase 1, cytoplasmic

Cat. Num. AAA9421130

• Gene Names: TXNRD1; TR; TR1; TXNR; TRXR1; GRIM-12
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Thioredoxin reductase 1; Recombinant Human Thioredoxin reductase 1; cytoplasmic; Gene associated with retinoic and interferon-induced mortality 12 protein; GRIM-12; Gene associated with retinoic and IFN-induced mortality 12 proteinKM-102-derived reductase-like factorThioredoxin reductase TR1; TRXR1 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Partial of isoform 5, 1-497aa

• Tag Info: His-SUMO-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for TRXR1 recombinant protein

Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell mbrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.

NCBI and Uniprot Product Information

• NCBI GI #: 148277071
• NCBI GeneID: 7296
• NCBI Accession #: NP_001087240.1
• NCBI GenBank Nucleotide #: NM_001093771.2
• UniProt Accession #: Q16881; Q6FI31; Q6VB40; Q6VB41; Q6VB42; Q6VBP2; Q6VBP3; B7Z1F4; B7Z3Y8; B7Z904; E9PMY9; F5H780
• Molecular Weight: 58.61kD
• NCBI Official Full Name: thioredoxin reductase 1, cytoplasmic isoform 5
• NCBI Official Synonym Full Names: thioredoxin reductase 1
• NCBI Official Symbol: TXNRD1
• NCBI Official Synonym Symbols: TR; TR1; TXNR; TRXR1; GRIM-12
• NCBI Protein Information: thioredoxin reductase 1, cytoplasmic
• UniProt Protein Name: Thioredoxin reductase 1, cytoplasmic
• Protein Family: Thioredoxin reductase
• UniProt Gene Name: TXNRD1
• UniProt Synonym Gene Names: GRIM12; KDRF; TR; GRIM-12; Gene associated with retinoic and IFN-induced mortality 12 protein

NCBI Description

The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms, including a glutaredoxin-containing isoform that is predominantly expressed in testis. [provided by RefSeq, May 2017]

Uniprot Description

TRXR1: a cytoplasmic pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine residue which is required for the catalytic activity.

Protein type: EC 1.8.1.9; Nuclear receptor co-regulator; Nucleotide Metabolism - pyrimidine; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q23.3

Cellular Component: cytosol; fibrillar center; mitochondrion; nucleoplasm

Molecular Function: protein binding; thioredoxin-disulfide reductase activity

Biological Process: cell redox homeostasis; nucleobase, nucleoside and nucleotide interconversion; regulation of lipid metabolic process; response to oxygen radical; selenium metabolic process