TUBB3 recombinant protein

Recombinant Human TUBB3 protein, C-His Tag

Cat. Num. AAA157711

• Gene Names: TUBB3; CDCBM; TUBB4; beta-4; CFEOM3A
• Purity: >90% as determined by SDS-PAGE
• Synonyms: TUBB3; Recombinant Human TUBB3 protein; C-His Tag; Tubulin beta-4 chain; Tubulin beta-III; TUBB4; TUBB3 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Source: Human
• Construction: A DNA sequence encoding the Human TUBB3 (Met1-Lys450) was fused with the C-terminal His Tag
• Preparation and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for ~12 months from the date of receipt.; Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.; In general, proteins are provided as lyophilized powder. They are shipped out with blue ice unless customers require otherwise

Product Categories/Family for TUBB3 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 308235963
• NCBI GeneID: 10381
• NCBI Accession #: NP_001184110.1
• NCBI GenBank Nucleotide #: NM_001197181.1
• UniProt Accession #: Q13509; Q9BTZ0; Q9BW10
• Molecular Weight: 50,433 Da
• NCBI Official Full Name: tubulin beta-3 chain isoform 2
• NCBI Official Synonym Full Names: tubulin, beta 3 class III
• NCBI Official Symbol: TUBB3
• NCBI Official Synonym Symbols: CDCBM; TUBB4; beta-4; CFEOM3A
• NCBI Protein Information: tubulin beta-3 chain; tubulin beta-III; tubulin beta-4 chain; class III beta-tubulin
• UniProt Protein Name: Tubulin beta-3 chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBB3
• UniProt Synonym Gene Names: TUBB4
• UniProt Entry Name: TBB3_HUMAN

NCBI Description

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

Uniprot Description

TUBB3: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Dimer of alpha and beta chains. Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: microtubule; cell soma; axon; cytoplasm; dendrite; nucleus

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; peptide binding

Biological Process: protein polymerization; axon guidance; mitosis; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; transmembrane transport; microtubule-based process

Disease: Fibrosis Of Extraocular Muscles, Congenital, 3a, With Or Without Extraocular Involvement; Cortical Dysplasia, Complex, With Other Brain Malformations 1