UQCRQ sirna

UQCRQ siRNA (Rat)

Cat. Num. AAA8218448

• Gene Names: Uqcrq; Qpc
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UQCRQ; UQCRQ siRNA (Rat); QPC; Cytochrome b-c1 complex subunit 8; Complex III subunit 8; Complex III subunit VIII; Low molecular mass ubiquinone-binding protein; Ubiquinol-cytochrome c reductase complex 9.5 kDa protein; Ubiquinol-cytochrome c reductase complex ubiquinone-binding protein QP-C; UQCRQ sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: UQCRQ siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 82

• Applicable Applications for UQCRQ sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat UQCRQ gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UQCRQ sirna

siRNA to inhibit UQCRQ expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 68341999
• NCBI GeneID: 497902
• NCBI Accession #: NP_001020305.1
• NCBI GenBank Nucleotide #: NM_001025134.1
• UniProt Accession #: Q7TQ16
• Molecular Weight: 9,849 Da
• NCBI Official Full Name: cytochrome b-c1 complex subunit 8
• NCBI Official Synonym Full Names: ubiquinol-cytochrome c reductase, complex III subunit VII
• NCBI Official Symbol: Uqcrq
• NCBI Official Synonym Symbols: Qpc
• NCBI Protein Information: cytochrome b-c1 complex subunit 8
• UniProt Protein Name: Cytochrome b-c1 complex subunit 8
• UniProt Gene Name: Uqcrq
• UniProt Synonym Gene Names: Qpc
• UniProt Entry Name: QCR8_RAT

Uniprot Description

UQCRQ: This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This subunit, together with cytochrome b, binds to ubiquinone. Defects in UQCRQ are a cause of mitochondrial complex III deficiency (MT-C3D). A disorder of the mitochondrial respiratory chain resulting in a highly variable phenotype depending on which tissues are affected. Clinical features include mitochondrial encephalopathy, psychomotor retardation, ataxia, severe failure to thrive, liver dysfunction, renal tubulopathy, muscle weakness and exercise intolerance. Belongs to the UQCRQ/QCR8 family.

Protein type: Membrane protein, integral; Oxidoreductase; Mitochondrial; Cell development/differentiation; Energy Metabolism - oxidative phosphorylation

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: ubiquinol-cytochrome-c reductase activity

Biological Process: hypothalamus development; pons development; hippocampus development; midbrain development; cerebellar Purkinje cell layer development; subthalamus development; pyramidal neuron development; thalamus development