SLC7A9 sirna

SLC7A9 siRNA (Rat)

Cat. Num. AAA8217611

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC7A9; SLC7A9 siRNA (Rat); BAT1; b(0.+)-type amino acid transporter 1; b(0.+)AT; Glycoprotein-associated amino acid transporter b0.+AT1; Solute carrier family 7 member 9; SLC7A9 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SLC7A9 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 487

• Applicable Applications for SLC7A9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SLC7A9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC7A9 sirna

siRNA to inhibit SLC7A9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758812
• NCBI GeneID: 116726
• NCBI Accession #: NP_446381.1
• NCBI GenBank Nucleotide #: NM_053929.1
• UniProt Accession #: P82252; Q4KM04
• Molecular Weight: 53,658 Da
• NCBI Official Full Name: B(0,+)-type amino acid transporter 1
• NCBI Official Synonym Full Names: solute carrier family 7 (amino acid transporter light chain, bo,+ system), member 9
• NCBI Official Symbol: Slc7a9
• NCBI Protein Information: B(0,+)-type amino acid transporter 1
• UniProt Protein Name: b(0,+)-type amino acid transporter 1
• Protein Family: b(0,+)-type amino acid transporter
• UniProt Gene Name: Slc7a9
• UniProt Synonym Gene Names: Bat1; b(0,+)AT
• UniProt Entry Name: BAT1_RAT

NCBI Description

amino acid transporter protein; exhibits high-affinity sodium-independent transport of cystine; may mediate reabsorption of cystine in the kidney proximal tubule [RGD, Feb 2006]

Uniprot Description

SLC7A9: Involved in the high-affinity, sodium-independent transport of cystine and neutral and dibasic amino acids (system b(0,+)-like activity). Thought to be responsible for the high- affinity reabsorption of cystine in the kidney tubule. Defects in SLC7A9 are a cause of non-type I cystinuria (CSNU). CSNU arises from impaired transport of cystine and dibasic amino acids through the epithelial cells of the renal tubule and gastrointestinal tract. Three types of cystinuria have been described: type I (fully recessive or silent); type II (high excretor); type III (moderate excretor). Defects in SLC7A9 are associated with type II and type III cystinuria. They also might account for some non-classic type I cystinuria cases. Belongs to the amino acid-polyamine-organocation (APC) superfamily.

Protein type: Transporter; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: brush border membrane; apical plasma membrane; integral to membrane; plasma membrane

Molecular Function: peptide antigen binding; neutral amino acid transmembrane transporter activity; L-cystine transmembrane transporter activity; amino acid transmembrane transporter activity

Biological Process: neutral amino acid transport; amino acid transport; L-cystine transport