ESR2 sirna

ESR2 siRNA (Rat)

Cat. Num. AAA8218482

• Gene Names: Esr2; Erb2; ERbeta; ER-beta
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ESR2; ESR2 siRNA (Rat); ERBETA; NR3A2; Estrogen receptor beta; ER-beta; Nuclear receptor subfamily 3 group A member 2; ESR2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ESR2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 485

• Applicable Applications for ESR2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ESR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ESR2 sirna

siRNA to inhibit ESR2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6978817
• NCBI GeneID: 25149
• NCBI Accession #: NP_036886.1
• NCBI GenBank Nucleotide #: NM_012754.1
• UniProt Accession #: Q62986; O35784; O35785; O55015; O55016; O70195; Q9R185
• Molecular Weight: 47,904 Da
• NCBI Official Full Name: estrogen receptor beta
• NCBI Official Synonym Full Names: estrogen receptor 2 (ER beta)
• NCBI Official Symbol: Esr2
• NCBI Official Synonym Symbols: Erb2; ERbeta; ER-beta
• NCBI Protein Information: estrogen receptor beta
• UniProt Protein Name: Estrogen receptor beta
• Protein Family: Estrogen receptor
• UniProt Gene Name: Esr2
• UniProt Synonym Gene Names: Erbeta; Nr3a2; ER-beta
• UniProt Entry Name: ESR2_RAT

NCBI Description

binds estrogen and mediates transcriptional activation [RGD, Feb 2006]

Uniprot Description

ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

Protein type: DNA-binding; Nuclear receptor

Cellular Component: neuron projection; cell soma; mitochondrion; perinuclear region of cytoplasm; cytoplasm; plasma membrane; perikaryon; nucleus; cilium

Molecular Function: ligand-dependent nuclear receptor activity; peroxisome proliferator activated receptor binding; estrogen receptor activity; hormone binding; zinc ion binding; estrogen response element binding; drug binding; steroid binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity

Biological Process: prostate gland development; hypothalamus development; estrogen receptor signaling pathway; behavioral fear response; negative regulation of smooth muscle cell proliferation; positive regulation of apoptosis; induction of apoptosis by hormones; positive regulation of transcription, DNA-dependent; response to hormone stimulus; neuron migration; negative regulation of transcription from RNA polymerase II promoter; amygdala development; response to insecticide; uterus development; response to organic cyclic substance; response to estradiol stimulus; negative regulation of cell proliferation; learning and/or memory; regulation of transcription, DNA-dependent; ovarian follicle development; positive regulation of epidermal growth factor receptor signaling pathway; regulation of neuron apoptosis; cerebellum development; female gonad development; negative regulation of epithelial cell proliferation; response to nutrient levels; steroid hormone receptor signaling pathway; response to drug; transcription, DNA-dependent; male gonad development; vagina development; Sertoli cell proliferation; response to testosterone stimulus; Sertoli cell development; regulation of cell proliferation; response to genistein; response to ethanol; response to estrogen stimulus; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; positive regulation of transcription factor activity; brain development; response to activity; negative regulation of behavior; response to water deprivation