ADCY8 sirna

ADCY8 siRNA (Rat)

Cat. Num. AAA8213011

• Gene Names: Adcy8; Ac8
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADCY8; ADCY8 siRNA (Rat); Adenylate cyclase type 8; ATP pyrophosphate-lyase 8; Adenylate cyclase type VIII; Adenylyl cyclase 8; Ca(2+)/calmodulin-activated adenylyl cyclase; ADCY8 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ADCY8 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1248

• Applicable Applications for ADCY8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ADCY8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADCY8 sirna

siRNA to inhibit ADCY8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8392852
• NCBI GeneID: 29241
• NCBI Accession #: NP_058838.1
• NCBI GenBank Nucleotide #: NM_017142.1
• UniProt Accession #: P40146
• Molecular Weight: 139,823 Da
• NCBI Official Full Name: adenylate cyclase type 8
• NCBI Official Synonym Full Names: adenylate cyclase 8 (brain)
• NCBI Official Symbol: Adcy8
• NCBI Official Synonym Symbols: Ac8
• NCBI Protein Information: adenylate cyclase type 8
• UniProt Protein Name: Adenylate cyclase type 8
• Protein Family: Adenylate cyclase
• UniProt Gene Name: Adcy8
• UniProt Entry Name: ADCY8_RAT

NCBI Description

may play a role in calcium/calmodulin regulated intracellular signaling; may mediate long term potentiation [RGD, Feb 2006]

Uniprot Description

ADCY8: This is a membrane-bound, calcium-stimulable adenylyl cyclase. May be involved in learning, in memory and in drug dependence. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Protein type: Adenylyl cyclase; Nucleotide Metabolism - purine; Transporter; Membrane protein, integral; EC 4.6.1.1; Lyase; Transporter, aquaporin family; Membrane protein, multi-pass

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: protein C-terminus binding; calmodulin binding; protein binding; metal ion binding; nucleotide binding; protein N-terminus binding; calcium- and calmodulin-responsive adenylate cyclase activity; ATP binding; adenylate cyclase activity

Biological Process: cAMP-mediated signaling; long-term memory; cAMP biosynthetic process; response to organic cyclic substance; G-protein signaling, adenylate cyclase activating pathway