TSHZ1 sirna

TSHZ1 siRNA (Mouse)

Cat. Num. AAA8209769

• Gene Names: Tshz1; Tsh1; Mtsh1; NY-CO-33; Sdccag33; mKIAA4206; teashirt1; D18Bwg1409e; 5730407I04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TSHZ1; TSHZ1 siRNA (Mouse); KIAA4206; SDCCAG33; TSH1; Teashirt homolog 1; Serologically defined colon cancer antigen 3 homolog; TSHZ1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TSHZ1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1084

• Applicable Applications for TSHZ1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TSHZ1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TSHZ1 sirna

siRNA to inhibit TSHZ1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 124486833
• NCBI GeneID: 110796
• NCBI Accession #: NP_001074769.1
• NCBI GenBank Nucleotide #: NM_001081300.1
• UniProt Accession #: Q5DTH5; Q6PE60; Q8VD19; Q9JLD5
• Molecular Weight: 118,567 Da
• NCBI Official Full Name: teashirt homolog 1
• NCBI Official Synonym Full Names: teashirt zinc finger family member 1
• NCBI Official Symbol: Tshz1
• NCBI Official Synonym Symbols: Tsh1; Mtsh1; NY-CO-33; Sdccag33; mKIAA4206; teashirt1; D18Bwg1409e; 5730407I04Rik
• NCBI Protein Information: teashirt homolog 1
• UniProt Protein Name: Teashirt homolog 1
• Protein Family: Teashirt
• UniProt Gene Name: Tshz1
• UniProt Synonym Gene Names: Kiaa4206; Sdccag33; Tsh1
• UniProt Entry Name: TSH1_MOUSE

Uniprot Description

TSHZ1: Probable transcriptional regulator involved in developmental processes. May act as a transcriptional repressor (Potential). Defects in TSHZ1 are the cause of congenital aural atresia (CAA). A rare anomaly of the ear that involves some degree of failure of the development of the external auditory canal. The malformation can also involve the tympanic membrane, ossicles and middle ear space. The inner ear development is most often normal. Different CAA forms are known. CAA type I is characterized by bony or fibrous atresia of the lateral part of the external auditory canal and an almost normal medial part and middle ear. CAA type II is the most frequent type and is characterized by partial or total aplasia of the external auditory canal. CAA type IIA involves an external auditory canal with either complete bony atresia of the medial part or partial aplasia that ends blindly in a fistula leading to a rudimentary tympanic membrane. CAA type IIB is characterized by bony stenosis of the total length of the external auditory canal. CAA type III involves bony atresia of the external auditory canal and a very small or absent middle-ear cavity. Belongs to the teashirt C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger protein

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding; metal ion binding

Biological Process: anterior/posterior pattern formation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; regulation of gene expression; multicellular organismal development; soft palate development; middle ear morphogenesis