MT-CYB sirna

MT-CYB siRNA (Mouse)

Cat. Num. AAA8206617

• Gene Names: mt-Cytb; CYTB
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MT-CYB; MT-CYB siRNA (Mouse); COB; CYTB; MT-CYTB; MTCYB; Cytochrome b; Complex III subunit 3; Complex III subunit III; Cytochrome b-c1 complex subunit 3; Ubiquinol-cytochrome-c reductase complex cytochrome b subunit; MT-CYB sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MT-CYB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 381

• Applicable Applications for MT-CYB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MT-CYB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MT-CYB sirna

siRNA to inhibit MT-CYB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 34538610
• NCBI GeneID: 17711
• NCBI Accession #: NP_904340.1
• NCBI GenBank Nucleotide #: NC_005089.1
• UniProt Accession #: P00158
• Molecular Weight: 43,210 Da
• NCBI Official Full Name: cytochrome b (mitochondrion)
• NCBI Official Synonym Full Names: cytochrome b, mitochondrial
• NCBI Official Symbol: mt-Cytb
• NCBI Official Synonym Symbols: CYTB
• NCBI Protein Information: cytochrome b
• UniProt Protein Name: Cytochrome b
• UniProt Gene Name: Mt-Cyb
• UniProt Synonym Gene Names: Cob; Cytb; mt-Cytb; Mtcyb
• UniProt Entry Name: CYB_MOUSE

Uniprot Description

CYTB: Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. Defects in MT-CYB are a rare cause of mitochondrial dysfunction underlying different myopathies. They include mitochondrial encephalomyopathy, hypertrophic cardiomyopathy (HCM), and sporadic mitochondrial myopathy (MM). In mitochondrial myopathy, exercise intolerance is the predominant symptom. Additional features include lactic acidosis, muscle weakness and/or myoglobinuria. Defects in MTCYB are also found in cases of exercise intolerance accompanied by deafness, mental retardation, retinitis pigmentosa, cataract, growth retardation, epilepsy (multisystem disorder). Defects in MT-CYB are the cause of cardiomyopathy infantile histiocytoid (CMIH). CMIH is characterized by the presence of pale granular foamy histiocyte-like cells within the myocardium. It usually affects children younger than 2 years of age, with a clear predominance of females over males. Infants present with dysrhythmia or cardiac arrest, and the clinical course is usually fulminant, sometimes simulating sudden infant death syndrome. Defects in MT-CYB contribute to Leber hereditary optic neuropathy (LHON). LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes. Belongs to the cytochrome b family.

Protein type: Membrane protein, integral; Energy Metabolism - oxidative phosphorylation; Membrane protein, multi-pass; Mitochondrial

Cellular Component: protein complex; mitochondrion; membrane; mitochondrial inner membrane; integral to membrane

Molecular Function: electron carrier activity; metal ion binding; protein complex binding; oxidoreductase activity

Biological Process: response to mercury ion; proton transport; response to cadmium ion; response to glucagon stimulus; hyperosmotic salinity response; transport; response to calcium ion