ESR1 sirna

ESR1 siRNA (Mouse)

Cat. Num. AAA8229442

• Gene Names: Esr1; ER; ERa; ESR; Estr; Estra; Nr3a1; ERalpha; ER-alpha
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ESR1; ESR1 siRNA (Mouse); ESR; ESTR; ESTRA; NR3A1; Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ESR1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 599

• Applicable Applications for ESR1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ESR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ESR1 sirna

siRNA to inhibit ESR1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 700274121
• NCBI GeneID: 13982
• NCBI Accession #: NP_001289460.1
• NCBI GenBank Nucleotide #: NM_001302531.1
• UniProt Accession #: P19785; Q9JJT5; Q9QY51; Q9QY52
• Molecular Weight: 66,955 Da
• NCBI Official Full Name: estrogen receptor isoform 1
• NCBI Official Synonym Full Names: estrogen receptor 1 (alpha)
• NCBI Official Symbol: Esr1
• NCBI Official Synonym Symbols: ER; ERa; ESR; Estr; Estra; Nr3a1; ERalpha; ER-alpha
• NCBI Protein Information: estrogen receptor
• UniProt Protein Name: Estrogen receptor
• Protein Family: Estrogen receptor
• UniProt Gene Name: Esr1
• UniProt Synonym Gene Names: Esr; Estr; Estra; Nr3a1; ER
• UniProt Entry Name: ESR1_MOUSE

NCBI Description

This gene encodes an estrogen receptor, a member of the nuclear hormone family of intracellular receptors. The encoded protein, activated by the sex hormone estrogen, is a transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Similar genes in human have been implicated in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Uniprot Description

ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Nuclear receptor

Cellular Component: Golgi apparatus; neuron projection; protein complex; mitochondrion; membrane; perinuclear region of cytoplasm; T-tubule; cytoplasm; plasma membrane; terminal button; perikaryon; cytosol; nucleus

Molecular Function: identical protein binding; ligand-dependent nuclear receptor activity; estrogen receptor activity; hormone binding; type 1 metabotropic glutamate receptor binding; zinc ion binding; metal ion binding; beta-catenin binding; estrogen response element binding; transcription factor binding; steroid binding; ATPase binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; protein complex binding; steroid hormone receptor activity; chromatin binding; lipid binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; estrogen receptor signaling pathway; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; uterus development; response to estradiol stimulus; regulation of apoptosis; elevation of cytosolic calcium ion concentration; positive regulation of fibroblast proliferation; regulation of transcription, DNA-dependent; positive regulation of epidermal growth factor receptor signaling pathway; regulation of neuron apoptosis; epithelial cell development; cell growth; steroid hormone receptor signaling pathway; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of nitric-oxide synthase activity; antral ovarian follicle growth; negative regulation of transcription factor activity; male gonad development; androgen metabolic process; vagina development; positive regulation of retinoic acid receptor signaling pathway; Sertoli cell proliferation; negative regulation of I-kappaB kinase/NF-kappaB cascade; regulation of transcription from RNA polymerase II promoter; negative regulation of glucose import; response to estrogen stimulus; negative regulation of mitosis; steroid hormone mediated signaling; positive regulation of transcription factor activity; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of transcription from RNA polymerase II promoter