CHUK sirna

CHUK siRNA (Mouse)

Cat. Num. AAA8207873

• Gene Names: Chuk; IKK1; Ikka; Chuk1; Fbx24; IKBKA; Fbxo24; NFKBIKA; AI256658
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHUK; CHUK siRNA (Mouse); IKKA; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; CHUK sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CHUK siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 584

• Applicable Applications for CHUK sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CHUK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHUK sirna

siRNA to inhibit CHUK expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 14285502
• NCBI GeneID: 12675
• UniProt Accession #: Q60680; Q80VU2; Q9D2X3
• Molecular Weight: 66,805 Da
• NCBI Official Full Name: Inhibitor of nuclear factor kappa-B kinase subunit alpha
• NCBI Official Synonym Full Names: conserved helix-loop-helix ubiquitous kinase
• NCBI Official Symbol: Chuk
• NCBI Official Synonym Symbols: IKK1; Ikka; Chuk1; Fbx24; IKBKA; Fbxo24; NFKBIKA; AI256658
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit alpha
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit alpha
• Protein Family: Inhibitor of nuclear factor kappa-B kinase
• UniProt Gene Name: Chuk
• UniProt Synonym Gene Names: Ikka; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA
• UniProt Entry Name: IKKA_MOUSE

Uniprot Description

IKKA: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B, leading to their dissociation from NF-kappa-B and ultimately to their degradation. Phosphorylated and activated downstream of growth factor receptors, IL1R, and TNFR by NAK, IRAK and NIK, respectively. Preferentially found as a heterodimer with IKK-beta but also as an homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. Heterodimers form the active complex. The tripartite complex can also bind to MAP3K14/NIK, MEKK1, IKAP and IKB-alpha-p65-p50 complex. Inhibitors are under development to treat arthritis, inflammation, and apoptotic aspects of cancer. Aspirin (sodium salicylate) selectively binds IKK and reduces inflammation. Misexpression and inhibitors show involvement in insulin sensitization in obese rodents. Mutations in IKK-gamma are found in several immune deficiencies: hyper IgM syndrome, incontinentia pigmenti [embryonic lethal in males, defects in skin, hair, teeth in females] and hypohidrotic ectodermal dysplasia; recurrent infections and defects in teeth, hair, and sweat glands]. Inhibitors: CHS 828, NBD peptide, BMS-345541.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; EC 2.7.11.10; Other group; IKK family

Cellular Component: nucleoplasm; internal side of plasma membrane; intracellular membrane-bound organelle; cytoplasm; IkappaB kinase complex; cytosol; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: lactation; positive regulation of I-kappaB kinase/NF-kappaB cascade; mammary gland epithelial cell proliferation; response to lipopolysaccharide; osteoclast differentiation; protein amino acid phosphorylation; Rho protein signal transduction; odontogenesis of dentine-containing teeth; response to organic substance; morphogenesis of an epithelial sheet; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; I-kappaB phosphorylation; phosphorylation