C8G sirna

C8G siRNA (Mouse)

Cat. Num. AAA8224813

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: C8G; C8G siRNA (Mouse); Complement component C8 gamma chain; C8G sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: C8G siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 202

• Applicable Applications for C8G sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse C8G gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for C8G sirna

siRNA to inhibit C8G expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 58037159
• NCBI GeneID: 69379
• NCBI Accession #: NP_081338.1
• NCBI GenBank Nucleotide #: NM_027062.2
• UniProt Accession #: Q8VCG4
• Molecular Weight: 22,508 Da
• NCBI Official Full Name: complement component C8 gamma chain isoform 1
• NCBI Official Synonym Full Names: complement component 8, gamma polypeptide
• NCBI Official Symbol: C8g
• NCBI Protein Information: complement component C8 gamma chain
• UniProt Protein Name: Complement component C8 gamma chain
• Protein Family: Complement component
• UniProt Gene Name: C8g
• UniProt Entry Name: CO8G_MOUSE

NCBI Description

This gene encodes a secreted protein that is a core component of the complement 8 (C8) complex. C8 is part of the membrane attack complex which participates in the innate immune response against bacterial pathogens. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]

Uniprot Description

C8G: C8 is a constituent of the membrane attack complex. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: membrane attack complex; extracellular space; extracellular region

Molecular Function: complement binding; protein complex binding; retinol binding

Biological Process: immune system process; cytolysis; complement activation, alternative pathway; innate immune response; complement activation, classical pathway