YES1 sirna

YES1 siRNA (Human)

Cat. Num. AAA8228209

• Gene Names: YES1; Yes; c-yes; HsT441; P61-YES
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: YES1; YES1 siRNA (Human); YES; Tyrosine-protein kinase Yes; Proto-oncogene c-Yes; p61-Yes; YES1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: YES1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 543

• Applicable Applications for YES1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human YES1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for YES1 sirna

siRNA to inhibit YES1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4885661
• NCBI GeneID: 7525
• NCBI Accession #: NP_005424.1
• NCBI GenBank Nucleotide #: NM_005433.3
• UniProt Accession #: P07947; A6NLB3; D3DUH1
• Molecular Weight: 60,801 Da
• NCBI Official Full Name: tyrosine-protein kinase Yes
• NCBI Official Synonym Full Names: YES proto-oncogene 1, Src family tyrosine kinase
• NCBI Official Symbol: YES1
• NCBI Official Synonym Symbols: Yes; c-yes; HsT441; P61-YES
• NCBI Protein Information: tyrosine-protein kinase Yes
• UniProt Protein Name: Tyrosine-protein kinase Yes
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: YES1
• UniProt Synonym Gene Names: YES
• UniProt Entry Name: YES_HUMAN

NCBI Description

This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]

Uniprot Description

Yes: a proto-oncogenic cytosolic tyrosine kinase of the Src family. Expressed at high levels in adult neurons, spermatozoa, platelets, and epithelial cells.

Protein type: Oncoprotein; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; TK group; Src family

Chromosomal Location of Human Ortholog: 18p11.31-p11.21

Cellular Component: Golgi apparatus; extrinsic to internal side of plasma membrane; focal adhesion; cytoplasm; plasma membrane; microtubule organizing center; actin filament; cytosol

Molecular Function: protein binding; enzyme binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding; epidermal growth factor receptor binding

Biological Process: axon guidance; T cell costimulation; ephrin receptor signaling pathway; protein modification process; innate immune response; glucose transport; regulation of vascular permeability; cell differentiation; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation