WARS2 sirna

WARS2 siRNA (Human)

Cat. Num. AAA8213285

• Gene Names: WARS2; TrpRS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WARS2; WARS2 siRNA (Human); Tryptophan--tRNA ligase mitochondrial; (Mt)TrpRS; Tryptophanyl-tRNA synthetase; TrpRS; WARS2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: WARS2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 360

• Applicable Applications for WARS2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WARS2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WARS2 sirna

siRNA to inhibit WARS2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7710154
• NCBI GeneID: 10352
• NCBI Accession #: NP_056651.1
• NCBI GenBank Nucleotide #: NM_015836.3
• UniProt Accession #: Q9UGM6; Q53FT4; Q5VUD2; Q86TQ0; B1ALR1; B2R9D4
• Molecular Weight: 24,813 Da
• NCBI Official Full Name: tryptophan--tRNA ligase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: tryptophanyl tRNA synthetase 2, mitochondrial
• NCBI Official Symbol: WARS2
• NCBI Official Synonym Symbols: TrpRS
• NCBI Protein Information: tryptophan--tRNA ligase, mitochondrial
• UniProt Protein Name: Tryptophan--tRNA ligase, mitochondrial
• UniProt Gene Name: WARS2
• UniProt Synonym Gene Names: TrpRS
• UniProt Entry Name: SYWM_HUMAN

NCBI Description

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. This gene encodes the mitochondrial tryptophanyl-tRNA synthetase. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

WARS2: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. This gene encodes the mitochondrial tryptophanyl-tRNA synthetase. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Protein type: Amino Acid Metabolism - tryptophan; Translation; Ligase; Mitochondrial; EC 6.1.1.2

Chromosomal Location of Human Ortholog: 1p12

Cellular Component: mitochondrial matrix

Molecular Function: tryptophan-tRNA ligase activity; ATP binding

Biological Process: tRNA aminoacylation for protein translation; tryptophanyl-tRNA aminoacylation; gene expression; vasculogenesis