PAOX sirna

PAOX siRNA (Human)

Cat. Num. AAA8225457

• Gene Names: PAOX; PAO
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PAOX; PAOX siRNA (Human); PAO; Peroxisomal N(1)-acetyl-spermine/spermidine oxidase; Polyamine oxidase; PAOX sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PAOX siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 511

• Applicable Applications for PAOX sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PAOX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PAOX sirna

siRNA to inhibit PAOX expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 23097272
• NCBI GeneID: 196743
• NCBI Accession #: NP_690875.1
• NCBI GenBank Nucleotide #: NM_152911.3
• UniProt Accession #: Q6QHF9; Q5VWY0; Q6QHF5; Q6QHF6; Q6QHF7; Q6QHF8; Q6QHG0; Q6QHG1; Q6QHG2; Q6QHG3; Q6QHG4; D3DXI6
• Molecular Weight: 4,499 Da
• NCBI Official Full Name: peroxisomal N(1)-acetyl-spermine/spermidine oxidase isoform 1
• NCBI Official Synonym Full Names: polyamine oxidase (exo-N4-amino)
• NCBI Official Symbol: PAOX
• NCBI Official Synonym Symbols: PAO
• NCBI Protein Information: peroxisomal N(1)-acetyl-spermine/spermidine oxidase
• UniProt Protein Name: Peroxisomal N(1)-acetyl-spermine/spermidine oxidase
• Protein Family: Peroxisomal N(1)-acetyl-spermine/spermidine oxidase
• UniProt Gene Name: PAOX
• UniProt Synonym Gene Names: PAO
• UniProt Entry Name: PAOX_HUMAN

Uniprot Description

PAOX: Flavoenzyme which catalyzes the oxidation of N(1)- acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N(1)-acetylspermidine to putrescine. Substrate specificity: N(1)-acetylspermine = N(1)- acetylspermidine > N(1),N(12)-diacylspermine >> spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. Belongs to the flavin monoamine oxidase family. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.5.3.13; Oxidoreductase

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: peroxisomal matrix

Molecular Function: polyamine oxidase activity; receptor binding

Biological Process: spermine catabolic process; xenobiotic metabolic process; putrescine biosynthetic process; polyamine biosynthetic process; polyamine catabolic process; spermidine catabolic process; putrescine catabolic process; polyamine metabolic process