C1QB sirna

C1QB siRNA (Human)

Cat. Num. AAA8225194

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: C1QB; C1QB siRNA (Human); Complement C1q subcomponent subunit B; C1QB sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: C1QB siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 253

• Applicable Applications for C1QB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human C1QB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for C1QB sirna

siRNA to inhibit C1QB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 87298828
• NCBI GeneID: 713
• NCBI Accession #: NP_000482.3
• NCBI GenBank Nucleotide #: NM_000491.3
• UniProt Accession #: P02746; Q5T959; Q96H17
• Molecular Weight: 26,722 Da
• NCBI Official Full Name: complement C1q subcomponent subunit B
• NCBI Official Synonym Full Names: complement component 1, q subcomponent, B chain
• NCBI Official Symbol: C1QB
• NCBI Protein Information: complement C1q subcomponent subunit B
• UniProt Protein Name: Complement C1q subcomponent subunit B
• Protein Family: Complement C1q subcomponent
• UniProt Gene Name: C1QB
• UniProt Entry Name: C1QB_HUMAN

NCBI Description

This gene encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the B-chain polypeptide of human complement subcomponent C1q [provided by RefSeq, Jul 2008]

Uniprot Description

C1QB: C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. Defects in C1QB are a cause of complement component C1q deficiency (C1QD). A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: collagen; extracellular region; complement component C1 complex

Molecular Function: protein binding; protein homodimerization activity

Biological Process: innate immune response; complement activation, classical pathway; inner ear development; complement activation

Disease: C1q Deficiency