ADCYAP1R1 sirna

ADCYAP1R1 siRNA (Human)

Cat. Num. AAA8213771

• Gene Names: ADCYAP1R1; PAC1; PAC1R; PACAPR; PACAPRI
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADCYAP1R1; ADCYAP1R1 siRNA (Human); Pituitary adenylate cyclase-activating polypeptide type I receptor; PACAP type I receptor; PACAP-R-1; PACAP-R1; ADCYAP1R1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ADCYAP1R1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 468

• Applicable Applications for ADCYAP1R1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADCYAP1R1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADCYAP1R1 sirna

siRNA to inhibit ADCYAP1R1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 29171759
• NCBI GeneID: 117
• NCBI Accession #: NP_001109.2
• NCBI GenBank Nucleotide #: NM_001118.4
• UniProt Accession #: P41586; Q17S10; A8K1Y1; B7ZLA7; B8ZZK3
• Molecular Weight: 46,957 Da
• NCBI Official Full Name: pituitary adenylate cyclase-activating polypeptide type I receptor isoform 3
• NCBI Official Synonym Full Names: adenylate cyclase activating polypeptide 1 (pituitary) receptor type I
• NCBI Official Symbol: ADCYAP1R1
• NCBI Official Synonym Symbols: PAC1; PAC1R; PACAPR; PACAPRI
• NCBI Protein Information: pituitary adenylate cyclase-activating polypeptide type I receptor
• UniProt Protein Name: Pituitary adenylate cyclase-activating polypeptide type I receptor
• Protein Family: Pituitary adenylate cyclase-activating polypeptide type I receptor
• UniProt Gene Name: ADCYAP1R1
• UniProt Synonym Gene Names: PACAP type I receptor; PACAP-R-1; PACAP-R1
• UniProt Entry Name: PACR_HUMAN

NCBI Description

This gene encodes type I adenylate cyclase activating polypeptide receptor, which is a membrane-associated protein and shares significant homology with members of the glucagon/secretin receptor family. This receptor mediates diverse biological actions of adenylate cyclase activating polypeptide 1 and is positively coupled to adenylate cyclase. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2010]

Uniprot Description

ADCYAP1R1: This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract. Belongs to the G-protein coupled receptor 2 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 2; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: neuron projection; tight junction; cell surface; rough endoplasmic reticulum; integral to plasma membrane; plasma membrane; caveola; receptor complex; endosome

Molecular Function: neuropeptide binding; ADP-ribosylation factor binding; vasoactive intestinal polypeptide receptor activity; protein binding; receptor activity; adenylate cyclase binding

Biological Process: response to drug; development of primary female sexual characteristics; nerve growth factor receptor signaling pathway; adenylate cyclase activation; response to estradiol stimulus; G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; cell surface receptor linked signal transduction; response to ethanol; phospholipase C activation; spermatogenesis; cell differentiation; multicellular organismal response to stress; transmembrane receptor protein tyrosine kinase signaling pathway