Suppressors Of Cytokine Signaling 3 (SOCS3) Recombinant Protein | SOCS3 recombinant protein

Recombinant Suppressors Of Cytokine Signaling 3 (SOCS3)

Cat. Num. AAA2010454

• Gene Names: Socs3; Cish3; Ssi-3; Socs-3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Suppressors Of Cytokine Signaling 3 (SOCS3); Recombinant Suppressors Of Cytokine Signaling 3 (SOCS3); SOCS3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-MVTHSKFPAA GMSRPLDTSL RLKTFSSKSE YQLVVNAVRK LQESGFYWSA VTGGEANLLL SAEPAGTFLI RDSSDQRHFF TLSVETQSGT KNLRIQCEGG SFSLQSDPRS TQPVPRFDCV LKLVHHYMPP PGAPSFSLPP TEPSFEVQEQ PPAQALPGGT PKRAYYIYSG GEKIPLVLSR PLSSNVATLQ HLCRKTVNGH LDSYEKVTQL PGPIREFLDQ YDAPL
• Sequence Length: 225

• Applicable Applications for SOCS3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Met1~Leu225 (Accession # O88583) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 16758336
• NCBI GeneID: 89829
• NCBI Accession #: NP_446017.1
• NCBI GenBank Nucleotide #: NM_053565.1
• UniProt Accession #: O88583; Q9QYV5
• Molecular Weight: 26.3kDa
• NCBI Official Full Name: suppressor of cytokine signaling 3
• NCBI Official Synonym Full Names: suppressor of cytokine signaling 3
• NCBI Official Symbol: Socs3
• NCBI Official Synonym Symbols: Cish3; Ssi-3; Socs-3
• NCBI Protein Information: suppressor of cytokine signaling 3; cytokine-inducible SH2 protein 3; cytokine inducible SH2-containing protein 3
• UniProt Protein Name: Suppressor of cytokine signaling 3
• Protein Family: Suppressor of cytokine signaling
• UniProt Gene Name: Socs3
• UniProt Synonym Gene Names: Cish3; SOCS-3
• UniProt Entry Name: SOCS3_RAT

NCBI Description

regulates cytokine levels to modulate inflammation [RGD, Feb 2006]

Uniprot Description

Function: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo. Probable substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST

By similarity.

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. Binding to JAK2 is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK2 JH1 domain. Binds specific activated tyrosine residues of the leptin, EPO, IL12, GSCF and gp130 receptors. Interaction with CSNK1E stabilize SOCS3 protein. Component of the probable ECS(SOCS3) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SOCS3. Interacts with CUL5, RNF7, TCEB1 and TCEB2. Interacts with FGFR3

By similarity. Interacts with INSR

By similarity. Interacts with BCL10; this interaction may interfere with BCL10-binding with PELI2

By similarity. Ref.3

Domain: The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes

By similarity.

Post-translational modification: Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1

By similarity.

Sequence similarities: Contains 1 SH2 domain.Contains 1 SOCS box domain.