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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
84 results for "retinoblastoma-family-protein" - showing 1-50
Western Blot (WB)
(Western blot analysis of anti-Cyclin D1 Antibody (S90) in mouse lung lysates (35ug/lane). Cyclin D1(arrow) was detected using the purified Pab.)
Cyclin D1, Polyclonal Antibody (Cat# AAA9214117)
Full Name
Cyclin D1 Antibody (S90)
Gene Names
CCND1; BCL1; PRAD1; U21B31; D11S287E
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
WB, EIA, IHC, IF
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot detection of eIF2alpha in K562, COS7, 3T3, C2C12, MCF-7, C6 and Hela cell lysates using eIF2alpha mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.)
eIF2alpha, Monoclonal Antibody (Cat# AAA475237)
Full Name
Anti-eIF2alpha Mouse mAb
Gene Names
EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
Reactivity
Human, Mouse, Rat
Applications
WB, IF, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot detection of RBBP5 in C2C12, THP-1, PC12 and 3T3 cell lysates using RBBP5 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
RBBP5, Monoclonal Antibody (Cat# AAA475202)
Full Name
Anti-RBBP5 Mouse mAb
Gene Names
RBBP5; RBQ3; SWD1
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Testing Data
(Cathepsin V (CTSV) is a lysosomal cysteine proteinase which belongs to peptidase C1 family. It is expressed in normal human thymus, testis and corneal epithelium. Cathepsin V plays an important role in corneal physiology and mediates degradation of invariant chain in human thymus. Besides, Retinoblastoma Protein 1 (RB1) has been identified as an interactor of CTSV, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CTSV and recombinant human RB1. Briefly, CTSV were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RB1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CTSV pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CTSV and RB1 was shown in Figure 1, and this effect was in a dose dependent manner.Figure 1.The binding activity of CTSV with RB1.)
Cathepsin V (CTSV), Active Protein (Cat# AAA2097309)
Full Name
Active Cathepsin V (CTSV)
Gene Names
CTSV; CTSU; CATL2; CTSL2
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>95%
Pricing
$225/0.01 mg | $485/0.05 mg | $755/0.1 mg | $930/0.2 mg | $1,820/0.5 mg | $2,690/1 mg | $6,580/5 mg
Western Blot (WB)
(Western blot detection of E2F-1 in C6, Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
E2F-1, Monoclonal Antibody (Cat# AAA475158)
Full Name
Anti-E2F-1 Mouse mAb
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human, Rat
Applications
WB, IF, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Sequence
Histone Deacetylase 1 (HDAC1), Active Protein (Cat# AAA2105171)
Full Name
Active Histone Deacetylase 1 (HDAC1)
Gene Names
HDAC1; HD1; RPD3; KDAC1; GON-10; RPD3L1
Reactivity
Homo sapiens (Human)
Applications
Cell Culture, Activity Assays
Purity
>95%
Pricing
$220/0.01 mg | $470/0.05 mg | $725/0.1 mg | $895/0.2 mg | $1,745/0.5 mg | $2,600/1 mg | $6,360/5 mg
Western Blot (WB)
(Western blot detection of Rb in Jurkat and MCF7 cell lysates using Rb mouse mAb (1:2000 diluted).Predicted band size:106KDa.Observed band size:110KDa.)
Rb, Monoclonal Antibody (Cat# AAA476491)
Full Name
Anti-Rb Mouse mAb
Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
Reactivity
Human
Applications
WB
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
Western Blot (WB)
(Western Blot Analysis of (1) HeLa (2) Jurkat and (3) MCF-7 cell lysates using Cyclin A1 Mouse Monoclonal Antibody (XLA1-3).)
Cyclin A1, Monoclonal Antibody (Cat# AAA4381478)
Full Name
Cyclin A1
Gene Names
ccna1.S; ccna1; Cyclin-A1
Reactivity
Xenopus laevis
Applications
WB
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Flow Cytometry
(Flow Cytometric analysis using HDAC1 antibodyFlow Cytometric analysis of HEK293T cells transfected with either recombinant HDAC1 protein (red) or empty vector (blue) stained using HDAC1 antibody)
HDAC1, Monoclonal Antibody (Cat# AAA830571)
Full Name
HDAC1 antibody
Gene Names
HDAC1; HD1; RPD3; GON-10; RPD3L1
Applications
FC/FACS, IF, WB
Purity
HDAC1 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
SDS-Page
(SDS-PAGE Analysis Purified Cyclin A Mouse Monoclonal Antibody (XLA1-1). Confirmation of Purity and Integrity of Antibody. )
Cyclin A1, Monoclonal Antibody (Cat# AAA4381584)
Full Name
Cyclin A1
Gene Names
ccna1.S; ccna1; Cyclin-A1
Reactivity
African clawed frog (Xenopus Laevis)
Applications
WB
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
CYCLIN D1, Polyclonal Antibody (Cat# AAA222202)
Full Name
RABBIT ANTI CYCLIN D1
Gene Names
CCND1; BCL1; PRAD1; U21B31; D11S287E
Reactivity
Amphibia (Expected from Sequence), Chimpanzee (Expected from Sequence), Dog (Expected from Sequence), Mouse, Rat, Zebrafish (Expected from Sequence)
Applications
EIA, WB
Pricing
$715/0.1 mL | $3,075/5x0.1 mL
Testing Data #1
(Western blot analysis of 25(A), 50(B) and 100(C) ng purified recombinant human nerve growth factor receptor (NGFR) with Rabbit anti Human CD271 (MBS221322))
CD271, Polyclonal Antibody (Cat# AAA221322)
Full Name
RABBIT ANTI HUMAN CD271
Gene Names
NGFR; CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
Reactivity
Mouse
Applications
WB
Pricing
$420/0.1 mg | $1,735/5x0.1 mg
Western Blot (WB)
E2F1, Polyclonal Antibody (Cat# AAA540238)
Full Name
E2F1 Antibody
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human, Mouse, Rat
Applications
EIA, IP, WB
Pricing
$490/0.1 mg | $2,065/5x0.1 mg
Western Blot (WB)
()
p130, Polyclonal Antibody (Cat# AAA837504)
Full Name
p130 antibody
Gene Names
RBL2; Rb2; P130
Applications
WB
Purity
p130 antibody was purified by Protein A affinity chromatography
chromosome 3 open reading frame 63, ELISA Kit (Cat# AAA9326549)
Full Name
Human Uncharacterized protein C3orf63, C3orf63 ELISA Kit
Gene Names
FAM208A; RAP140; se89-1; C3orf63
Reactivity
Human
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Western Blot (WB)
E2F1, Polyclonal Antibody (Cat# AAA540274)
Full Name
E2F1 Antibody Affinity Purified
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$490/0.1 mg | $2,065/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HDAC1 protein (Right) or empty vector (Left) detected with HDAC1 antibody)
HDAC1, Monoclonal Antibody (Cat# AAA533927)
Full Name
HDAC1 antibody
Gene Names
HDAC1; HD1; RPD3; GON-10; RPD3L1
Applications
FC/FACS, IF, WB
Purity
HDAC1 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
HIV1p27Kip1, Polyclonal Antibody (Cat# AAA535317)
Full Name
HIV1p27Kip1 antibody
Reactivity
Human
Applications
IHC
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HDAC1 protein (Right) or empty vector (Left) detected with HDAC1 antibody)
HDAC1, Monoclonal Antibody (Cat# AAA830938)
Full Name
HDAC1 antibody
Gene Names
HDAC1; HD1; RPD3; GON-10; RPD3L1
Applications
FC/FACS, IF, WB
Purity
HDAC1 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Testing Data #1
(Western blot analysis of mouse colon lysate probed with Rabbit anti Human CDKN2A (MBS222939) at 1(A) and 2(B) ug/ml)
CDKN2A, Polyclonal Antibody (Cat# AAA222939)
Full Name
RABBIT ANTI CDKN2A
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Mouse, Rat
Applications
WB
Pricing
$435/0.1 mg | $1,800/5x0.1 mg
USP4, Polyclonal Antibody (Cat# AAA535459)
Full Name
USP4 antibody
Gene Names
USP4; UNP; Unph
Reactivity
Human
Applications
IHC
Purity
USP4 antibody was purified by affinity chromatography.
Pricing
$885/0.05 mg | $3,965/5x0.05 mg
USP4, Polyclonal Antibody (Cat# AAA535582)
Full Name
USP4 antibody
Gene Names
USP4; UNP; Unph
Reactivity
Human
Applications
IHC
Purity
USP4 antibody was purified by affinity chromatography.
Pricing
$885/0.05 mg | $3,965/5x0.05 mg
CDKN2A, Polyclonal Antibody (Cat# AAA221351)
Full Name
GOAT ANTI HUMAN CDKN2A (C-TERMINAL)
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Applications
EIA, WB
Pricing
$625/0.1 mg | $2,670/5x0.1 mg
Testing Data
(IP western blot analysis was performed on human leukemia cell lines that harbor MLL chromosomal translocations and express MLL chimeric oncoproteins (indicated at tops). IP was performed with the antibodies indicated on the top of the gels, and the precipitates were immunoblotted with the antibodies indicated on the right. Antibody MBS190886 is shown as AF4. Anti-GST antibodies served as negative controls. Positive control: K562 cell lysates.Optimal dilutions/concentrations should be determined by the end user.)
AF4, Monoclonal Antibody (Cat# AAA190886)
Full Name
Anti-AF4 Monoclonal Antibody
Gene Names
AFF1; AF4; PBM1; MLLT2
Applications
IB
Purity
Purified by Protein G
Pricing
$405/0.1 mg | $1,760/5x0.1 mg
Retinoblastoma-related protein (RBL901), Recombinant Protein (Cat# AAA1229415)
Full Name
Recombinant Populus trichocarpa Retinoblastoma-related protein (RBL901), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
E2F1, Positive Control (Cat# AAA542717)
Full Name
E2F1 Positive Control
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Applications
WB
Pricing
$335/5 Applications | $1,350/5x5 Applications
E2F1, Polyclonal Antibody (Cat# AAA541445)
Full Name
E2F1 Antibody Affinity Purified
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$490/0.1 mg | $2,065/5x0.1 mg
E2F2, Positive Control (Cat# AAA542718)
Full Name
E2F2 Antibody Western Blot Positive Control
Gene Names
E2f2; E2F-2
Applications
WB
Pricing
$335/5 Applications | $1,350/5x5 Applications
Western Blot (WB)
(The anti-RB Pab is used in Western blot to detect RB in, from left to right, HL60, T47D, and SK-BR-3 tissue lysates.)
Phospho-RB (S612), Polyclonal Antibody (Cat# AAA9209781)
Full Name
Phospho-RB (S612) Antibody
Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
Reactivity
Human
Applications
WB, EIA, IHC-p
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$435/0.4 mL | $190/0.08 mL | $1,705/5x0.4 mL
MRFAP1, Polyclonal Antibody (Cat# AAA839219)
Full Name
MRFAP1 antibody
Applications
EIA, IHC, WB
Purity
MRFAP1 antibody was purified by Antigen Affinity chromatography
Pricing
$400/0.05 mL | $1,780/5x0.05 mL
Retinoblastoma family protein (Rbf), Recombinant Protein (Cat# AAA1374913)
Full Name
Recombinant Drosophila melanogaster Retinoblastoma family protein (Rbf), partial
Gene Names
Rbf; CG7413; DmelCG7413; dRBF; EG:34F3.3; FBF; pRb; rb; Rb; RB; Rb1; rbf; RbF; RBF; rbf1; Rbf1; RBf1; RBF1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
E2F1, Blocking Peptide (Cat# AAA543605)
Full Name
E2F1 Antibody Antigenic Blocking Peptide
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Applications
Immunocompetition, Immunodepletion
Pricing
$280/0.25 mg | $1,110/5x0.25 mg
E2F1, Polyclonal Antibody (Cat# AAA541449)
Full Name
E2F1 Antibody FITC-Conjugated
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
E2F1, Polyclonal Antibody (Cat# AAA541450)
Full Name
E2F1 Antibody BIOTIN-Conjugated
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
E2F1, Polyclonal Antibody (Cat# AAA541448)
Full Name
E2F1 Antibody BIOTIN-Conjugated
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
E2F1, Blocking Peptide (Cat# AAA543607)
Full Name
E2F1 Peptide
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Applications
Immunocompetition, Immunodepletion
Pricing
$280/0.25 mg | $1,110/5x0.25 mg
CCND1, Polyclonal Antibody (Cat# AAA1490299)
Full Name
Rabbit Anti Human CCND1 PolyClonal Antibody
Gene Names
CCND1; BCL1; PRAD1; U21B31; D11S287E
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IP
Purity
Affinity Purification
Pricing
$320/0.1 mL | $1,440/5x0.1 mL
E2F1, Polyclonal Antibody (Cat# AAA541451)
Full Name
E2F1 Antibody FITC-Conjugated
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
E2F1, Blocking Peptide (Cat# AAA543606)
Full Name
E2F1 Antibody Antigenic Blocking Peptide
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Applications
Immunocompetition, Immunodepletion
Pricing
$280/0.25 mg | $1,110/5x0.25 mg
E2F1, Polyclonal Antibody (Cat# AAA541447)
Full Name
E2F1 Antibody FITC-Conjugated
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
E2F1, Polyclonal Antibody (Cat# AAA541446)
Full Name
E2F1 Antibody BIOTIN-Conjugated
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
FAM208A, siRNA (Cat# AAA8232964)
Full Name
FAM208A siRNA (Human)
Gene Names
FAM208A; RAP140; se89-1; C3orf63
Reactivity
Human
Applications
RNAi
Purity
> 97%
Pricing
$395/15 nmol | $555/30 nmol | $2,440/5x30 nmol
Western Blot (WB)
(Western blot analysis of Rb expression in Jurkat cell lysate treated with Alkaline Phosphatase (MBS179051).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RB1 )
Rb, Monoclonal Antibody (Cat# AAA179051)
Full Name
Anti-Rb Rabbit Monoclonal Antibody
Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
Reactivity
Human
Applications
IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse spleen, using Phospho-Rb (S807) Antibody (MBS1750198)RB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RB1 Antibody (MBS1750198)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Rb, Monoclonal Antibody (Cat# AAA1750198)
Full Name
Anti-Phospho-Rb (S807) Rabbit Monoclonal Antibody
Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of E2F1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate (MBS179227).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E2F1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for E2F1 )
E2F1, Monoclonal Antibody (Cat# AAA179227)
Full Name
Anti-E2F1 Rabbit Monoclonal Antibody
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(HMX1 antibody - middle region validated by WB using Y79, WERI-(human retinoblastoma cell lines), HeLa-(human papilloma virus cell line) at 1:1000.)
HMX1, Polyclonal Antibody (Cat# AAA3201058)
Full Name
HMX1 antibody - middle region
Gene Names
HMX1; H6; NKX5-3
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
FRK, Recombinant Protein (Cat# AAA5304551)
Full Name
FRK protein
Purity
FRK protein was purified using a a proprietary chromatographic technique.
Pricing
$540/0.005 mg | $2,425/5x0.005 mg
Western Blot (WB)
(Western blot analysis of Rb expression in (1) Jurkat cell lysate; (2) MCF-7 cell lysate (MBS179052).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RB1 )
Rb, Monoclonal Antibody (Cat# AAA179052)
Full Name
Anti-Rb Rabbit Monoclonal Antibody
Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
Reactivity
Human, Mouse
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
NDN / Necdin, Recombinant Protein (Cat# AAA2901762)
Full Name
Human NDN / Necdin Recombinant Protein
Gene Names
NDN; PWCR; HsT16328
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
Western Blot (WB)
(Figure 1. Western blot analysis of SERPINF1 using anti-SERPINF1 antibody (M02034).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINF1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SERPINF1. )
PEDF, Monoclonal Antibody (Cat# AAA1752161)
Full Name
Anti-PEDF Monoclonal Antibody
Gene Names
SERPINF1; OI6; OI12; PEDF; EPC-1; PIG35
Reactivity
Human
Applications
FC/FACS, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
