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Gene Name
Isotype
Host
Application
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10000 results for " phosphorylation" - showing 4900-4950
Ikk alpha, beta, Polyclonal Antibody (Cat# AAA624470)
Full Name
Ikk alpha, beta (IkappaB Kinase alpha, beta, IKKa,b)
Gene Names
IKBKB; IKK2; IKKB; NFKBIKB; IKK-beta
Reactivity
Human, Mouse, Rat
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunD (Ab-255) antibody.)
JunD, Antibody (Cat# AAA856686)
Full Name
JunD (Ab-255) Antibody
Gene Names
JUND; AP-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of K562 using p-TIRAP (Y86) antibody.)
TIRAP, Polyclonal Antibody (Cat# AAA9414567)
Full Name
TIRAP (Phospho-Tyr86) Polyclonal Antibody
Gene Names
TIRAP; Mal; wyatt; BACTS1; MyD88-2
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of 293T cells using Phospho-Tak1 (T187) Polyclonal Antibody)
Tak1, Polyclonal Antibody (Cat# AAA9404733)
Full Name
Tak1 (Phospho-Thr187) Polyclonal Antibody
Gene Names
MAP3K7; TAK1; MEKK7; TGF1a
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Testing Data
MAP Kinase Kinase 3, Polyclonal Antibody (Cat# AAA625081)
Full Name
MAP Kinase Kinase 3, phosphorylated (Ser189) (Dual Specificity Mitogen-activated Protein Kinase Kinase 3, Mitogen-activated Protein Kinase Kinase 3, MAP2K3, MAPKK 3, MKK3, MAPK/ERK Kinase 3, MEK 3, MEK3, PRKMK3, Stress-activated Protein Kinase Kinase 2, S
Gene Names
MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3
Reactivity
Human
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$620/0.1 mg | $2,780/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts from HuvEc, using C-RAF (Phospho-Ser301) antibody. The lane on the right is treated with the synthesized peptide.)
C-RAF, Polyclonal Antibody (Cat# AAA9408687)
Full Name
C-RAF (Phospho-Ser301) Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using Connexin 43 (Phospho-Ser261) antibody.)
Connexin 43, Antibody (Cat# AAA859538)
Full Name
Connexin 43 (Phospho-Ser261) Antibody
Gene Names
GJA1; HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; EKVP3; HLHS1; PPKCA
Reactivity
Human
Applications
IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data
(P-peptide-+)
MAPKAPK2, Antibody (Cat# AAA852291)
Full Name
MAPKAPK2 (Phospho-Ser272) Antibody
Gene Names
MAPKAPK2; MK2; MK-2; MAPKAP-K2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HER2 (phospho-Tyr877) antibody.)
HER2, Antibody (Cat# AAA856935)
Full Name
HER2 (Phospho-Tyr877) Antibody
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorrylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data
COXIV (Ser58), Polyclonal Antibody (Cat# AAA502194)
Full Name
Anti-Phospho-Ser58 COXIV
Gene Names
Cox4i1; Cox4; COXIV; Cox4a; AL024441
Reactivity
Mouse, rat
Applications
WB
Purity
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Pricing
$420/0.1 mL | $1,740/5x0.1 mL
Pyk2, Polyclonal Antibody (Cat# AAA626772)
Full Name
Pyk2, phosphorylated (Tyr402) (Protein-tyrosine Kinase 2-beta, Calcium-dependent Tyrosine Kinase, CADTK, Cell Adhesion Kinase beta, CAK-beta, Focal Adhesion Kinase 2, FADK 2, Proline-rich Tyrosine Kinase 2, Related Adhesion Focal Tyrosine Kinase, RAFTK, P
Gene Names
PTK2B; PKB; PTK; CAKB; FAK2; PYK2; CADTK; FADK2; RAFTK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Western Blot (WB)
(Western blot analysis of TGF beta receptor 1 in lysates from rat kindey (Lane 1), 3T3 cells (Lane 2) and Jurkat cells (Lane 3 & 4).)
TGFR1, Polyclonal Antibody (Cat# AAA837241)
Full Name
TGFR1 antibody
Applications
WB
Purity
TGFR1 antibody was purified by affinity chromatography
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using Bcr (Ab-177) antibody.)
Bcr, Antibody (Cat# AAA858931)
Full Name
Bcr (Ab-177) Antibody
Gene Names
BCR; ALL; CML; PHL; BCR1; D22S11; D22S662
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain tissue using a-Synuclein(Phospho-Tyr136) Antibody and the same antibody preincubated with blocking peptide.)
alpha-Synuclein, Polyclonal Antibody (Cat# AAA9413608)
Full Name
alpha-Synuclein (Phospho-Tyr136) Antibody
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from RAW264.7 cells, treated with forskolin (40nM, 30mins), using Kir5.1 (Phospho-Ser417) antibody.)
Kir5.1, Antibody (Cat# AAA858554)
Full Name
Kir5.1 (Phospho-Ser417) Antibody
Gene Names
KCNJ16; BIR9; KIR5.1
Reactivity
Mouse, Rat
Applications
WB, IHC, IF, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunofluorescence (IF)
(Immunocytochemistry of fixed and permeabilized MDCK cells using biotinylated alpha Tubulin antibody followed by Streptavidin FITC.)
alpha Tubulin, Monoclonal Antibody (Cat# AAA833243)
Full Name
alpha Tubulin antibody (biotin)
Applications
FC/FACS
Pricing
$385/0.025 mg | $1,725/5x0.025 mg
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, treated with EGF (200ng/ml, 5mins), using Androgen Receptor (Ab-363) antibody.)
Androgen Receptor, Antibody (Cat# AAA851423)
Full Name
Androgen Receptor (Ab-363) Antibody
Gene Names
AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded breast carcinoma, using p53 (Phospho-Ser15) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.)
p53, Antibody (Cat# AAA853869)
Full Name
p53 (Phospho-Ser15) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Rat
Applications
IHC, EIA, WB, IP
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot All lanes: Tumor necrosis factor antibody at 2/mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 26 kDa Observed band size: 60 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Tumor necrosis factor, Polyclonal Antibody (Cat# AAA719434)
Full Name
Rabbit anti-Bovine Tumor necrosis factor polyclonal Antibody, FITC
Gene Names
TNF; TNFa; TNF-a; TNF-alpha
Reactivity
Bovine
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data
(P-Peptide - +Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin E1 (phospho-Thr395) Antibody (E011541).)
Cyclin E1, Antibody (Cat# AAA857253)
Full Name
Cyclin E1 (phospho-Thr395) Antibody
Gene Names
CCNE1; CCNE; pCCNE1
Reactivity
Human
Applications
IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
PHOSPHOSERINE, Polyclonal Antibody (Cat# AAA222020)
Full Name
RABBIT ANTI PHOSPHOSERINE
Applications
EIA, IP, WB
Pricing
$715/0.05 mg | $3,075/5x0.05 mg
Western Blot (WB)
(Western blot analysis of EIF2AK1 Antibody (Center) in Hela cell line lysates (35ug/lane). EIF2AK1 (arrow) was detected using the purified Pab.)
HRI (EIF2AK1), Polyclonal Antibody (Cat# AAA9206335)
Full Name
HRI (EIF2AK1) Antibody (Center)
Gene Names
EIF2AK1; HCR; HRI
Reactivity
Human
Applications
WB, EIA, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts from JK cells (Lane 2), using TAL-1 (Phospho-Ser122) Antibody. The lane on the left is treated with antigen-specific peptide.)
TAL-1, Polyclonal Antibody (Cat# AAA9402398)
Full Name
TAL-1 (Phospho-Ser122) Antibody
Gene Names
TAL1; SCL; TCL5; tal-1; bHLHa17
Reactivity
Human, Mouse
Applications
WB
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Phosphorylation P38 Mitogen-Activated Protein Kinase, ELISA Kit (Cat# AAA10659)
Full Name
Fish Phosphorylation P38 Mitogen-Activated Protein Kinase ELISA Kit
Reactivity
Fish
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Histone H3 (Phospho-Thr3) antibody (#A0434).)
Histone H3, Antibody (Cat# AAA854834)
Full Name
Histone H3 (Phospho-Thr3) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from JK cells using AML1 (Phospho-Ser276) Antibody.The lane on the left is treated with the antigen-specific peptide.)
AML1, Polyclonal Antibody (Cat# AAA9401992)
Full Name
AML1 (Phospho-Ser276) Antibody
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Structure
Baricitinib, Inhibitor (Cat# AAA576687)
Full Name
Baricitinib (LY3009104, INCB028050)
Purity
98.9%
Pricing
$185/2 mg | $195/5 mg | $205/10 mg | $230/25 mg | $245/50 mg | $270/100 mg | $195/1 mL (in DMSO) | $950/5x100 mg
Western Blot (WB)
(Western blot analysis of 293T using p-Tak1 (S439) antibody.)
Tak1, Polyclonal Antibody (Cat# AAA9405083)
Full Name
Tak1 (Phospho-Ser439) Polyclonal Antibody
Gene Names
MAP3K7; TAK1; MEKK7; TGF1a
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from 293 cells using SOX-9 (Phospho-Ser181) Antibody.The lane on the right is treated with the antigen-specific peptide.)
SOX-9, Polyclonal Antibody (Cat# AAA9405676)
Full Name
SOX-9 (Phospho-Ser181) Antibody
Gene Names
SOX9; CMD1; SRA1; CMPD1; SRXX2; SRXY10
Reactivity
Human, Mouse
Applications
WB
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from rat heart, using PAK3 (Ab-154) antibody.)
PAK3, Antibody (Cat# AAA855781)
Full Name
PAK3 (Ab-154) Antibody
Gene Names
PAK3; ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3cells, using c-RAF (Phospho-Ser43) antibody.)
C-RAF, Antibody (Cat# AAA8501000)
Full Name
C-RAF (Phospho-Ser43) Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform Antibody at 2 ug/ml + EC109 whole cell lysate at 20 ug Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 34 kDa Observed band size: 45 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, Polyclonal Antibody (Cat# AAA715317)
Full Name
Rabbit anti-human Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform polyclonal Antibody, FITC
Gene Names
PPP2CB; PP2CB; PP2Abeta
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Elk1, Polyclonal Antibody (Cat# AAA628161)
Full Name
Elk1, phosphorylated (Ser383) (ETS Domain-containing Protein Elk-1)
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Western Blot (WB)
(Western blot analysis of extracts from COS7 cells treated with UV using Chk2 (Phospho-Thr383) Antibody.The lane on the right is treated with the antigen-specific peptide.)
Chk2, Polyclonal Antibody (Cat# AAA9407563)
Full Name
Chk2 (Phospho-Thr383) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western blot analysis of p44/42 MAP Kinase (Tyr204) in NIH-3T3 cells.)
MAP Kinase p44/42, Polyclonal Antibody (Cat# AAA6001191)
Full Name
MAP Kinase p44/42, phosphorylated (Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinase 2, MA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$580/0.05 mg | $2,600/5x0.05 mg
Western Blot (WB)
(Western blot All lanes: Activator of 90 kDa heat shock protein ATPase homolog 1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 37 kDa Observed band size: 45kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) · multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Activator of 90 kDa heat shock protein ATPase homolog 1, Polyclonal Antibody (Cat# AAA715985)
Full Name
Rabbit anti-human Activator of 90 kDa heat shock protein ATPase homolog 1 polyclonal Antibody, HRP conjugated
Gene Names
AHSA1; p38; AHA1; C14orf3
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
SDS-Page
(Peptide Competition Extracts of NIH3T3 cells untreated (lane 1) or treated with 50 ng/mL PDGF for 15 minutes (lanes 2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 4% BSA-TBST buffer for one hour at room temperature, then incubated with the MEK1 [pT292] antibody in a 1% BSA-TBST buffer for two hours at RT following prior incubation with: no peptide (1, 2), a generic phosphothreonine-containing peptide (3), the nonphosphopeptide corresponding to the phosphopeptide immunogen (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and signals were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to MEK1 [pT292] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the induction of MEK1 [pT292] phosphorylation by the addition of PDGF to this cell system. In addition, this antibody did not recognize a recombinant MEK1 T292A mutant protein (kindly provided by Dr. Natalie Ahn, University of Colorado), further demonstrating its specificity for MEK1 [pT292] (data not shown).)
MEK1, Polyclonal Antibody (Cat# AAA627915)
Full Name
MEK1, phosphorylated (Thr292) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-Activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1)
Gene Names
MEK1; ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK
Reactivity
Human, Mouse
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$695/10 Blots | $3,110/5x10 Blots
Western Blot (WB)
(Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30min) using Ezrin (Ab-566) antibody and Ezrin (phospho-Thr566) antibody.)
Ezrin, Antibody (Cat# AAA857558)
Full Name
Ezrin (Ab-566) Antibody
Gene Names
EZR; CVL; CVIL; VIL2; HEL-S-105
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
AMPK1, 2, Polyclonal Antibody (Cat# AAA625033)
Full Name
AMPK1, 2 (5'-AMP-activated Protein Kinase Catalytic Subunit alpha-1, AMPK Subunit alpha-1, PRKAA1/5'-AMP-activated Protein Kinase Catalytic Subunit alpha-2, AMPK Subunit alpha-2, PRKAA2)
Reactivity
Human, Mouse, Rat
Applications
IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Western Blot (WB)
(Western Blot analysis of extracts from Hela cells untreated or treated with starvationusing alpha-catenin (Phospho-Ser641) antibody)
alpha catenin, Polyclonal Antibody (Cat# AAA5302856)
Full Name
alpha catenin antibody
Gene Names
CTNNA1; CAP102
Applications
WB
Purity
alpha catenin antibody was purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
Testing Data
(P-peptide-+)
MAP2K3, Antibody (Cat# AAA855687)
Full Name
MAP2K3 (Phospho-Thr222) Antibody
Gene Names
MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
SDS-Page
(Figure. SDS-PAGE)
Complement Receptor 2 (CR2), Recombinant Protein (Cat# AAA2018463)
Full Name
Recombinant Complement Receptor 2 (CR2)
Gene Names
Cr2; Cr1; C3DR; CD21; CD35; Cr-1; Cr-2
Reactivity
Mus musculus (Mouse)
Applications
WB
Purity
> 97%
Pricing
$165/0.01 mg | $315/0.05 mg | $485/0.1 mg | $595/0.2 mg | $1,145/0.5 mg | $1,695/1 mg | $4,160/5 mg
ATP synthase, H+ transportingF0 complex, subunit F6, Polyclonal Antibody (Cat# AAA714654)
Full Name
Rabbit anti-human ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 polyclonal Antibody
Gene Names
ATP5J; F6; CF6; ATP5; ATPM; ATP5A
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Antigen Affinity purified
Pricing
$250/0.05 mL | $540/0.15 mL | $2,410/5x0.15 mL
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT5A (Ab-694) antibody.)
STAT5A, Antibody (Cat# AAA856983)
Full Name
STAT5A (Ab-694) Antibody
Gene Names
STAT5A; MGF; STAT5
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using Zap-70 (Phospho-Tyr319) antibody)
Zap 70, Polyclonal Antibody (Cat# AAA5301214)
Full Name
Zap 70 antibody
Applications
IF, IHC, WB
Purity
Zap 70 antibody was purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
AMPK1, 2, Polyclonal Antibody (Cat# AAA630212)
Full Name
AMPK1, 2 (5'-AMP-activated Protein Kinase Catalytic Subunit alpha-1, AMPK Subunit alpha-1, PRKAA1/5'-AMP-activated Protein Kinase Catalytic Subunit alpha-2, AMPK Subunit alpha-2, PRKAA2)
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (Ab-727) antibody.)
STAT3, Antibody (Cat# AAA857166)
Full Name
STAT3 (Ab-727) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKC theta (Ab-676) antibody.)
PKC theta, Antibody (Cat# AAA856944)
Full Name
PKC theta (Ab-676) Antibody
Gene Names
PRKCQ; PRKCT; nPKC-theta
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Ab-421) antibody (MBS857476).)
p70 S6 Kinase, Antibody (Cat# AAA857476)
Full Name
p70 S6 Kinase (Ab-421) Antibody
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human, Mouse, Rat
Applications
IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from A549 cells and NIH-3T3 cells using K6PL antibody)
K6PL, Polyclonal Antibody (Cat# AAA5302981)
Full Name
K6PL antibody
Applications
WB, EIA
Pricing
$405/0.1 mg | $1,805/5x0.1 mg
