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3255 results for "Primary Antibodies Detection antibody" - showing 2300-2350
Western Blot (WB)
(Western blot analysis of Rat kidney lysate showing detection of ~28 kDa MMP7 protein using Rabbit Anti-MMP7 Polyclonal Antibody (SPC-735). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat kidney lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-MMP7 Polyclonal Antibody (SPC-735) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~28 kDa.)
MMP7, Polyclonal Antibody (Cat# AAA8000661)
Full Name
MMP7 Antibody
Gene Names
MMP7; MMP-7; MPSL1; PUMP-1
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse brain lysate showing detection of ~32.5 kDa Cyclin D3 protein using Rabbit Anti-Cyclin D3 Polyclonal Antibody (SPC-732). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclin D3 Polyclonal Antibody (SPC-732) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~32.5 kDa. Other Band(s): ~45 kDa due to post translational modifications.)
Cyclin D3, Polyclonal Antibody (Cat# AAA8000608)
Full Name
Cyclin D3 antibody: ATTO 390
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat kidney lysate showing detection of ~28 kDa MMP7 protein using Rabbit Anti-MMP7 Polyclonal Antibody (SPC-735). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat kidney lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-MMP7 Polyclonal Antibody (SPC-735) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~28 kDa.)
MMP7, Polyclonal Antibody (Cat# AAA8000663)
Full Name
MMP7 Antibody: ATTO 488
Gene Names
MMP7; MMP-7; MPSL1; PUMP-1
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of ~18.3 kDa BAD protein using Rabbit Anti-BAD Polyclonal Antibody (SPC-773). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-BAD Polyclonal Antibody (SPC-773) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~18.3 kDa. Other Band(s): 28 kDa (PTM).)
BAD, Polyclonal Antibody (Cat# AAA8001346)
Full Name
BAD Antibody: ATTO 390
Gene Names
BAD; BBC2; BCL2L8
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse brain lysate showing detection of ~32.5 kDa Cyclin D3 protein using Rabbit Anti-Cyclin D3 Polyclonal Antibody (SPC-732). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclin D3 Polyclonal Antibody (SPC-732) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~32.5 kDa. Other Band(s): ~45 kDa due to post translational modifications.)
Cyclin D3, Polyclonal Antibody (Cat# AAA8000616)
Full Name
Cyclin D3 antibody: Alkaline Phosphatase
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~33.1 kDa Cyclin D2 protein using Rabbit Anti-Cyclin D2 Polyclonal Antibody (SPC-733). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclin D2 Polyclonal Antibody (SPC-733) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~33.1 kDa. Other Band(s): ~50 kDa due to post translational modifications.)
Cyclin D2, Polyclonal Antibody (Cat# AAA8000638)
Full Name
Cyclin D2 antibody: HRP
Gene Names
CCND2; MPPH3; KIAK0002
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 52.9 kDa ATG4D protein using Rabbit Anti-ATG4D Polyclonal Antibody (SPC-635). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody (SPC-635) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 52.9 kDa. Other Band(s): ~45 and 48 kDa.)
ATG4D, Polyclonal Antibody (Cat# AAA809616)
Full Name
ATG4D Antibody
Gene Names
ATG4D; APG4D; AUTL4; APG4-D
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 48.1 kDa Beclin 2 protein using Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-604). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-604) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 48.1 kDa.)
Beclin 2, Polyclonal Antibody (Cat# AAA809412)
Full Name
Beclin 2 Antibody: FITC
Gene Names
Becn2; Gm104; Beclin-2
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,900/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Pancreas cell lysates showing detection of 46.2kDa VMP1 protein using Rabbit Anti-VMP1 Polyclonal Antibody (SPC-680). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody (SPC-680) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 46.2kDa. Other Band(s): Multiple bands for A549; Rat Brain shows a band at 60kDa, 75kDa, and 150kDa.)
VMP1, Polyclonal Antibody (Cat# AAA8000007)
Full Name
VMP1 Antibody: FITC
Gene Names
VMP1; EPG3; TANGO5; TMEM49
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,900/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of ~41.9 kDa CCR2 protein using Rabbit Anti-CCR2 Polyclonal Antibody (SPC-704). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CCR2 Polyclonal Antibody (SPC-704) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~41.9 kDa.)
CCR2, Polyclonal Antibody (Cat# AAA8000263)
Full Name
CCR2 Antibody: RPE
Gene Names
CCR2; CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.)
SMAD4, Polyclonal Antibody (Cat# AAA8000572)
Full Name
SMAD4 Antibody: ATTO 390
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~70 kDa HSP70 protein using Llama Anti-HSP70 Polyclonal Antibody (SPC-744). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Llama Anti-HSP70 Polyclonal Antibody (SPC-744) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Llama HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.)
HSP70, Polyclonal Antibody (Cat# AAA8000825)
Full Name
HSP70 Antibody: ATTO 488
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
Reactivity
Human; Mouse; Rat
Applications
WB, EIA
Purity
Peptide Affinity Purified
Pricing
$495/0.1 mg | $2,010/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~41 kDa Wnt1 protein using Rabbit Anti-Wnt1 Polyclonal Antibody (SPC-759). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Wnt1 Polyclonal Antibody (SPC-759) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~41 kDa. Other Band(s): Band is detected at ~46 kDa, due to post translational modifications.)
Wnt1, Polyclonal Antibody (Cat# AAA8001105)
Full Name
Wnt1 Antibody: FITC
Gene Names
WNT1; INT1; OI15; BMND16
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~41 kDa Wnt1 protein using Rabbit Anti-Wnt1 Polyclonal Antibody (SPC-759). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Wnt1 Polyclonal Antibody (SPC-759) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~41 kDa. Other Band(s): Band is detected at ~46 kDa, due to post translational modifications.)
Wnt1, Polyclonal Antibody (Cat# AAA8001108)
Full Name
Wnt1 Antibody: PerCP
Gene Names
WNT1; INT1; OI15; BMND16
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of ~43.4 kDa MEK1 protein using Rabbit Anti-MEK1 Polyclonal Antibody (SPC-779). Lane 1: Molecular Weight Ladder (MW). Lane 2: A459. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-MEK1 Polyclonal Antibody (SPC-779) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~43.4 kDa.)
MEK1, Polyclonal Antibody (Cat# AAA8001464)
Full Name
MEK1 Antibody: Biotin
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.)
SMAD4, Polyclonal Antibody (Cat# AAA8000575)
Full Name
SMAD4 Antibody: ATTO 594
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~104.7 kDa ROR2 protein using Rabbit Anti-ROR2 Polyclonal Antibody (SPC-739). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ROR2 Polyclonal Antibody (SPC-739) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~104.7 kDa. Other Band(s): ~60, 35 kDa degradation products.)
ROR2, Polyclonal Antibody (Cat# AAA8000737)
Full Name
ROR2 Antibody: ATTO 594
Gene Names
ROR2; BDB; BDB1; NTRKR2
Reactivity
Human; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~104.7 kDa ROR2 protein using Rabbit Anti-ROR2 Polyclonal Antibody (SPC-739). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ROR2 Polyclonal Antibody (SPC-739) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~104.7 kDa. Other Band(s): ~60, 35 kDa degradation products.)
ROR2, Polyclonal Antibody (Cat# AAA8000749)
Full Name
ROR2 Antibody: RPE
Gene Names
ROR2; BDB; BDB1; NTRKR2
Reactivity
Human; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~41 kDa Wnt1 protein using Rabbit Anti-Wnt1 Polyclonal Antibody (SPC-759). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Wnt1 Polyclonal Antibody (SPC-759) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~41 kDa. Other Band(s): Band is detected at ~46 kDa, due to post translational modifications.)
Wnt1, Polyclonal Antibody (Cat# AAA8001094)
Full Name
Wnt1 Antibody: ATTO 390
Gene Names
WNT1; INT1; OI15; BMND16
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Pancreas cell lysates showing detection of 46.2kDa VMP1 protein using Rabbit Anti-VMP1 Polyclonal Antibody (SPC-680). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody (SPC-680) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 46.2kDa. Other Band(s): Multiple bands for A549; Rat Brain shows a band at 60kDa, 75kDa, and 150kDa.)
VMP1, Polyclonal Antibody (Cat# AAA8000011)
Full Name
VMP1 Antibody: RPE
Gene Names
VMP1; EPG3; TANGO5; TMEM49
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Pancreas cell lysates showing detection of 45.3 kDa ATG4A protein using Rabbit Anti-ATG4A Polyclonal Antibody (SPC-628). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody (SPC-628) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 45.3 kDa. Other Band(s): ~55-60 kDa.)
ATG4A, Polyclonal Antibody (Cat# AAA809530)
Full Name
ATG4A Antibody: ATTO 594
Gene Names
ATG4A; APG4A; AUTL2
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Liver showing detection of ~16.5, 11.2 kDa CDKN2A protein using Rabbit Anti-CDKN2A Polyclonal Antibody (SPC-775). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CDKN2A Polyclonal Antibody (SPC-775) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16.5, 11.2 kDa.)
CDKN2A, Polyclonal Antibody (Cat# AAA8001385)
Full Name
CDKN2A Antibody: ATTO 594
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of ~21.6 kDa KRAS protein using Rabbit Anti-KRAS Polyclonal Antibody (SPC-777). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-KRAS Polyclonal Antibody (SPC-777) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21.6 kDa. Other Band(s): ~29 kDa (PTM).)
KRAS, Polyclonal Antibody (Cat# AAA8001421)
Full Name
KRAS Antibody: ATTO 594
Gene Names
KRAS; NS; NS3; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody . Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
SFRP2, Polyclonal Antibody (Cat# AAA8001591)
Full Name
SFRP2 Antibody: FITC
Gene Names
SFRP2; FRP-2; SARP1; SDF-5
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~41.6 kDa TCF7 protein using Rabbit Anti-TCF7 Polyclonal Antibody (SPC-724). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF7 Polyclonal Antibody (SPC-724) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~41.6 kDa. Other Band(s): ~54 kDa isoform.)
TCF7, Polyclonal Antibody (Cat# AAA8000478)
Full Name
TCF7 Antibody: PerCP
Gene Names
TCF7; TCF-1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48.1 kDa SMAD3 protein using Rabbit Anti-SMAD3 Polyclonal Antibody (SPC-729). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD3 Polyclonal Antibody (SPC-729) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.1 kDa. Other Band(s): due to post translational modifications.)
SMAD3, Polyclonal Antibody (Cat# AAA8000555)
Full Name
SMAD3 Antibody: ATTO 488
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse, Rat liver lysate showing detection of ~52.7 kDa Presenilin 1 protein using Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-734). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse liver lysate. Lane 3: Rat liver lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-734) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~52.7 kDa. Other Band(s): 28, 32, 42 kDa other isoforms.)
Presenilin 1, Polyclonal Antibody (Cat# AAA8000658)
Full Name
Presenilin 1 Antibody: PerCP
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~90 kDa HSP90 alpha/beta protein using Llama Anti-HSP90 alpha/beta Polyclonal Antibody (SPC-745). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Llama Anti-HSP90 alpha/beta Polyclonal Antibody (SPC-745) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Llama HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~90 kDa.)
HSP90 alpha/beta, Polyclonal Antibody (Cat# AAA8000854)
Full Name
HSP90 alpha/beta Antibody: HRP
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
Reactivity
Human; Mouse; Rat
Applications
WB, EIA
Purity
Peptide Affinity Purified
Pricing
$485/0.1 mg | $1,950/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808389)
Full Name
N-terminal Arginylation Antibody, Clone 4A9: HRP
Reactivity
Species Independent
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$570/0.1 mg | $2,320/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 183 kDa FIP200 protein using Rabbit Anti-FIP200 Polyclonal Antibody (SPC-658). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FIP200 Polyclonal Antibody (SPC-658) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 183 kDa.)
FIP200, Polyclonal Antibody (Cat# AAA809833)
Full Name
FIP200 Antibody: ATTO 390
Gene Names
RB1CC1; CC1; ATG17; FIP200; PPP1R131
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG10 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG10 Antibody . (D) Composite.)
ATG10, Polyclonal Antibody (Cat# AAA809938)
Full Name
ATG10 Antibody: RPE
Gene Names
ATG10; APG10; APG10L; pp12616
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse, Rat liver lysate showing detection of ~52.7 kDa Presenilin 1 protein using Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-734). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse liver lysate. Lane 3: Rat liver lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-734) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~52.7 kDa. Other Band(s): 28, 32, 42 kDa other isoforms.)
Presenilin 1, Polyclonal Antibody (Cat# AAA8000656)
Full Name
Presenilin 1 Antibody: HRP
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~42.3 kDa Wnt5a protein using Rabbit Anti-Wnt5a Polyclonal Antibody (SPC-737). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Wnt5a Polyclonal Antibody (SPC-737) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~42.3 kDa. Other Band(s): ~35 kDa isoform 2.)
Wnt5a, Polyclonal Antibody (Cat# AAA8000699)
Full Name
Wnt5a Antibody: ATTO 488
Gene Names
WNT5A; hWNT5A
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse testis lysate showing detection of ~59.9 kDa FZD4 protein using Rabbit Anti-FZD4 Polyclonal Antibody (SPC-738). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse testis lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FZD4 Polyclonal Antibody (SPC-738) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~59.9 kDa.)
FZD4, Polyclonal Antibody (Cat# AAA8000716)
Full Name
FZD4 Antibody: ATTO 390
Gene Names
FZD4; Fz4; EVR1; FEVR; Fz-4; FzE4; GPCR; hFz4; CD344; FZD4S
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of ~43 kDa ROR1 protein using Rabbit Anti-ROR1 Polyclonal Antibody (SPC-763). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ROR1 Polyclonal Antibody (SPC-763) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~43 kDa. Other Band(s): Isoform 3.)
ROR1, Polyclonal Antibody (Cat# AAA8001174)
Full Name
ROR1 Antibody: Alkaline Phosphatase
Gene Names
ROR1; NTRKR1; dJ537F10.1
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of ~43 kDa ROR1 protein using Rabbit Anti-ROR1 Polyclonal Antibody (SPC-763). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ROR1 Polyclonal Antibody (SPC-763) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~43 kDa. Other Band(s): Isoform 3.)
ROR1, Polyclonal Antibody (Cat# AAA8001176)
Full Name
ROR1 Antibody: Biotin
Gene Names
ROR1; NTRKR1; dJ537F10.1
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse, Rat Brain showing detection of ~43.1, 38.2, 40.1 kDa ERK1 protein using Rabbit Anti-ERK1 Polyclonal Antibody (SPC-780). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Lane 3: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (SPC-780) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~43.1, 38.2, 40.1 kDa. Other Band(s): 50 kDa.)
ERK1, Polyclonal Antibody (Cat# AAA8001484)
Full Name
ERK1 Antibody: HRP
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of ~48.1 kDa SMAD3 protein using Rabbit Anti-SMAD3 Polyclonal Antibody (SPC-754). Lane 1: Molecular Weight Ladder (MW). Lane 2: A549. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD3 Polyclonal Antibody (SPC-754) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.1 kDa.)
SMAD3, Polyclonal Antibody (Cat# AAA8001016)
Full Name
SMAD3 Antibody: HRP
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Liver showing detection of ~16.5, 11.2 kDa CDKN2A protein using Rabbit Anti-CDKN2A Polyclonal Antibody (SPC-775). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CDKN2A Polyclonal Antibody (SPC-775) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16.5, 11.2 kDa.)
CDKN2A, Polyclonal Antibody (Cat# AAA8001382)
Full Name
CDKN2A Antibody: ATTO 390
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse, Rat Brain showing detection of ~43.1, 38.2, 40.1 kDa ERK1 protein using Rabbit Anti-ERK1 Polyclonal Antibody (SPC-780). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Lane 3: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (SPC-780) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~43.1, 38.2, 40.1 kDa. Other Band(s): 50 kDa.)
ERK1, Polyclonal Antibody (Cat# AAA8001471)
Full Name
ERK1 Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-PDGFB Polyclonal Antibody . Tissue: Heart. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-PDGFB Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.)
PDGFB, Polyclonal Antibody (Cat# AAA8001689)
Full Name
PDGFB Antibody: ATTO 488
Gene Names
PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GAPDH Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GAPDH Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GAPDH Antibody. (D) Composite.)
GAPDH, Polyclonal Antibody (Cat# AAA8000116)
Full Name
GAPDH Antibody: HRP
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~41.6 kDa TCF7 protein using Rabbit Anti-TCF7 Polyclonal Antibody (SPC-724). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF7 Polyclonal Antibody (SPC-724) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~41.6 kDa. Other Band(s): ~54 kDa isoform.)
TCF7, Polyclonal Antibody (Cat# AAA8000467)
Full Name
TCF7 Antibody: ATTO 594
Gene Names
TCF7; TCF-1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48.1 kDa SMAD3 protein using Rabbit Anti-SMAD3 Polyclonal Antibody (SPC-729). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD3 Polyclonal Antibody (SPC-729) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.1 kDa. Other Band(s): due to post translational modifications.)
SMAD3, Polyclonal Antibody (Cat# AAA8000554)
Full Name
SMAD3 Antibody: ATTO 390
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~21 kDa RAC1 protein using Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T . Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa.)
RAC1, Polyclonal Antibody (Cat# AAA8001070)
Full Name
RAC1 Antibody: HRP
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 288.9 kDa MTOR protein using Rabbit Anti-MTOR Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-MTOR Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 288.9 kDa. Other Band(s): 25 kDa.)
mTOR, Polyclonal Antibody (Cat# AAA8001796)
Full Name
mTOR Antibody: ATTO 390
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat kidney lysate showing detection of ~67.9 kDa TCF7L2 protein using Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat kidney lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.9 kDa. Other Band(s): ~55 kDa isoform, ~75 kDa due to post translational modification.)
TCF7L2, Polyclonal Antibody (Cat# AAA8000442)
Full Name
TCF7L2 Antibody: PerCP
Gene Names
TCF7L2; TCF4; TCF-4
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
7-Ketocholesterol, Monoclonal Antibody (Cat# AAA808749)
Full Name
7-Ketocholesterol Antibody, Clone 3F7: HRP
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$545/0.1 mg | $2,215/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 112.6 kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 112.6 kDa.)
ULK1, Polyclonal Antibody (Cat# AAA809688)
Full Name
ULK1 Antibody
Gene Names
ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse liver lysate showing detection of ~71.3 kDa TCF4 protein using Rabbit Anti-TCF4 Polyclonal Antibody (SPC-723). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse liver lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF4 Polyclonal Antibody (SPC-723) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71.3 kDa. Other Band(s): ~45 kDa isoform.)
TCF4, Polyclonal Antibody (Cat# AAA8000461)
Full Name
TCF4 Antibody: RPE
Gene Names
TCF4; E2-2; ITF2; PTHS; SEF2; FECD3; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; bHLHb19
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
