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3255 results for "Primary Antibodies Detection antibody" - showing 2050-2100
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GAPDH Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GAPDH Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GAPDH Antibody. (D) Composite.)
GAPDH, Polyclonal Antibody (Cat# AAA8000104)
Full Name
GAPDH Antibody: ATTO 390
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48.8 kDa c-Myc protein using Rabbit Anti-c-Myc Polyclonal Antibody (SPC-725). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Myc Polyclonal Antibody (SPC-725) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.8 kDa.)
c-Myc, Polyclonal Antibody (Cat# AAA8000481)
Full Name
c-Myc Antibody
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48.8 kDa c-Myc protein using Rabbit Anti-c-Myc Polyclonal Antibody (SPC-725). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Myc Polyclonal Antibody (SPC-725) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.8 kDa.)
c-Myc, Polyclonal Antibody (Cat# AAA8000490)
Full Name
c-Myc Antibody: Alkaline Phosphatase
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~134.3 kDa EGFR protein using Rabbit Anti-EGFR Polyclonal Antibody (SPC-772). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-EGFR Polyclonal Antibody (SPC-772) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~134.3 kDa. Other Band(s): 44 kDa and 69 kDa.)
EGFR, Polyclonal Antibody (Cat# AAA8001327)
Full Name
EGFR Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL2 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL2 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GABARAPL2 Antibody. (D) Composite.)
GABARAPL2, Polyclonal Antibody (Cat# AAA809499)
Full Name
GABARAPL2 Antibody: Alkaline Phosphatase
Gene Names
GABARAPL2; ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of 53.4 kDa ULK3 protein using Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 53.4 kDa. Other Band(s): ~25 kDa in all lysates.)
ULK3, Polyclonal Antibody (Cat# AAA809740)
Full Name
ULK3 Antibody: RPE
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL2 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL2 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GABARAPL2 Antibody. (D) Composite.)
GABARAPL2, Polyclonal Antibody (Cat# AAA809503)
Full Name
GABARAPL2 Antibody: HRP
Gene Names
GABARAPL2; ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human Serum starved HeLa cells treated with PMA showing detection of 41 kDa c-fos protein using Mouse Anti-c-fos Monoclonal Antibody, Clone 5B11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Serum starved HeLa cell lysates. Lane 3: Serum starved HeLa cells treated with PMA (Phorbol 12-myristate 13-acetate). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-c-fos Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 41 kDa. Other Band(s): 62 kDa. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.)
c-Fos, Monoclonal Antibody (Cat# AAA809199)
Full Name
c-Fos Antibody, Clone 5B11: HRP
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Human
Applications
WB
Purity
Protein G Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human Serum starved HeLa cells treated with PMA showing detection of 41 kDa c-fos protein using Mouse Anti-c-fos Monoclonal Antibody, Clone 5B11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Serum starved HeLa cell lysates. Lane 3: Serum starved HeLa cells treated with PMA (Phorbol 12-myristate 13-acetate). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-c-fos Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 41 kDa. Other Band(s): 62 kDa. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.)
c-Fos, Monoclonal Antibody (Cat# AAA809201)
Full Name
c-Fos Antibody, Clone 5B11: PerCP
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Human
Applications
WB
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human Serum starved HeLa cells treated with PMA showing detection of 41 kDa c-fos protein using Mouse Anti-c-fos Monoclonal Antibody, Clone 12G11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Serum starved HeLa cell lysates. Lane 3: Serum starved HeLa cells treated with PMA (Phorbol 12-myristate 13-acetate). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-c-fos Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 41 kDa. Other Band(s): 62 kDa. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.)
c-Fos, Monoclonal Antibody (Cat# AAA809206)
Full Name
c-Fos Antibody, Clone 12G11: ATTO 488
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Human
Applications
WB
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody (SPC-743). Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 uM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody (SPC-743) at 1:60 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) HSP70 Antibody (Acetyl Lys77). (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
HSP70, Polyclonal Antibody (Cat# AAA8000820)
Full Name
HSP70 Antibody (Acetyl Lys77): PerCP
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
Reactivity
Human; Mouse; Rat
Applications
WB, EIA, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$560/0.1 mL | $2,270/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Trypanosoma brucei brucei cells showing detection of ~71 kDa TbHsp70 protein using Rabbit Anti-TbHsp70 Polyclonal Antibody (SPC-747). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cells incubated for 1 hour at 37 degree C. Lane 3: Cells incubated for 1 hour at 42 degree C. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TbHsp70 Polyclonal Antibody (SPC-747) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71 kDa.)
TbHsp70, Polyclonal Antibody (Cat# AAA8000892)
Full Name
TbHsp70 Antibody: PerCP
Reactivity
Trypanosoma brucei brucei
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4. Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.)
ENaC alpha, Monoclonal Antibody (Cat# AAA808279)
Full Name
ENaC alpha Antibody, Clone 2G4: Biotin
Gene Names
Scnn1a; ENaCA
Reactivity
Mouse; Rat
Applications
WB, IHC
Purity
Protein G Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human 293T showing detection of 28.7 kDa DKK1 protein using Rabbit Anti-DKK1 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: 293T . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-DKK1 Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 28.7 kDa.)
DKK1, Polyclonal Antibody (Cat# AAA8001610)
Full Name
DKK1 Antibody: HRP
Gene Names
DKK1; SK; DKK-1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~28.7 kDa PCNA protein using Rabbit Anti-PCNA Polyclonal Antibody (SPC-691). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (SPC-691) at 1:1000 for 1 hour. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 1 hour RT with shaking. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~28.7 kDa.)
PCNA, Polyclonal Antibody (Cat# AAA8000122)
Full Name
PCNA Antibody: ATTO 390
Gene Names
PCNA; ATLD2
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~28.7 kDa PCNA protein using Rabbit Anti-PCNA Polyclonal Antibody (SPC-691). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (SPC-691) at 1:1000 for 1 hour. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 1 hour RT with shaking. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~28.7 kDa.)
PCNA, Polyclonal Antibody (Cat# AAA8000133)
Full Name
PCNA Antibody: FITC
Gene Names
PCNA; ATLD2
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,900/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~28.7 kDa PCNA protein using Rabbit Anti-PCNA Polyclonal Antibody (SPC-691). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (SPC-691) at 1:1000 for 1 hour. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 1 hour RT with shaking. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~28.7 kDa.)
PCNA, Polyclonal Antibody (Cat# AAA8000137)
Full Name
PCNA Antibody: RPE
Gene Names
PCNA; ATLD2
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse brain lysate showing detection of ~32.5 kDa Cyclin D3 protein using Rabbit Anti-Cyclin D3 Polyclonal Antibody (SPC-732). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclin D3 Polyclonal Antibody (SPC-732) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~32.5 kDa. Other Band(s): ~45 kDa due to post translational modifications.)
Cyclin D3, Polyclonal Antibody (Cat# AAA8000607)
Full Name
Cyclin D3 antibody
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 1F2. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
KDEL, Monoclonal Antibody (Cat# AAA809071)
Full Name
KDEL Antibody, Clone 1F2: Biotin
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Protein G Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-FUNDC1 Polyclonal Antibody . Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-FUNDC1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.)
FUNDC1, Polyclonal Antibody (Cat# AAA809683)
Full Name
FUNDC1 Antibody: HRP
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat brain lysate showing detection of ~33.7 kDa Cyclin D1 protein using Rabbit Anti-Cyclin D1 Polyclonal Antibody (SPC-731). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclin D1 Polyclonal Antibody (SPC-731) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~33.7 kDa. Other Band(s): ~40 kDa due to post translational modifications.)
Cyclin D1, Polyclonal Antibody (Cat# AAA8000605)
Full Name
Cyclin D1 antibody: RPE
Gene Names
CCND1; BCL1; PRAD1; U21B31; D11S287E
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~35.7 kDa c-Jun protein using Rabbit Anti-c-Jun Polyclonal Antibody (SPC-753). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Jun Polyclonal Antibody (SPC-753) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~35.7 kDa.)
c-Jun, Polyclonal Antibody (Cat# AAA8000994)
Full Name
c-Jun Antibody: Alkaline Phosphatase
Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.)
SMAD4, Polyclonal Antibody (Cat# AAA8000571)
Full Name
SMAD4 Antibody
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse brain lysate showing detection of ~55.7 kDa PIK3CA protein using Rabbit Anti-PIK3CA Polyclonal Antibody (SPC-741). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3CA Polyclonal Antibody (SPC-741) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa.)
PIK3CA, Polyclonal Antibody (Cat# AAA8000781)
Full Name
PIK3CA Antibody: FITC
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~41 kDa Wnt1 protein using Rabbit Anti-Wnt1 Polyclonal Antibody (SPC-759). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Wnt1 Polyclonal Antibody (SPC-759) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~41 kDa. Other Band(s): Band is detected at ~46 kDa, due to post translational modifications.)
Wnt1, Polyclonal Antibody (Cat# AAA8001104)
Full Name
Wnt1 Antibody: Biotin
Gene Names
WNT1; INT1; OI15; BMND16
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~35.7 kDa c-Jun protein using Rabbit Anti-c-Jun Polyclonal Antibody (SPC-753). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Jun Polyclonal Antibody (SPC-753) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~35.7 kDa.)
c-Jun, Polyclonal Antibody (Cat# AAA8001001)
Full Name
c-Jun Antibody: RPE
Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 52.9 kDa ATG4D protein using Rabbit Anti-ATG4D Polyclonal Antibody (SPC-635). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody (SPC-635) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 52.9 kDa. Other Band(s): ~45 and 48 kDa.)
ATG4D, Polyclonal Antibody (Cat# AAA809620)
Full Name
ATG4D Antibody: ATTO 594
Gene Names
ATG4D; APG4D; AUTL4; APG4-D
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSF2 Polyclonal Antibody (SPC-712). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-HSF2 Polyclonal Antibody (SPC-712) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) HSF2 Antibody. (D) Composite.)
HSF2, Polyclonal Antibody (Cat# AAA8000328)
Full Name
HSF2 Antibody: Alkaline Phosphatase
Gene Names
HSF2; HSF 2; HSTF 2
Reactivity
Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSF2 Polyclonal Antibody (SPC-712). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-HSF2 Polyclonal Antibody (SPC-712) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) HSF2 Antibody. (D) Composite.)
HSF2, Polyclonal Antibody (Cat# AAA8000332)
Full Name
HSF2 Antibody: HRP
Gene Names
HSF2; HSF 2; HSTF 2
Reactivity
Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse brain lysate showing detection of ~55.7 kDa PIK3CA protein using Rabbit Anti-PIK3CA Polyclonal Antibody (SPC-741). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3CA Polyclonal Antibody (SPC-741) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa.)
PIK3CA, Polyclonal Antibody (Cat# AAA8000778)
Full Name
PIK3CA Antibody: Alkaline Phosphatase
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~70 kDa HSP70 protein using Llama Anti-HSP70 Polyclonal Antibody (SPC-744). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Llama Anti-HSP70 Polyclonal Antibody (SPC-744) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Llama HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.)
HSP70, Polyclonal Antibody (Cat# AAA8000836)
Full Name
HSP70 Antibody: HRP
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
Reactivity
Human; Mouse; Rat
Applications
WB, EIA
Purity
Peptide Affinity Purified
Pricing
$485/0.1 mg | $1,950/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~27.3 kDa PDGFB protein using Rabbit Anti-PDGFB Polyclonal Antibody (SPC-770). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PDGFB Polyclonal Antibody (SPC-770) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~27.3 kDa.)
PDGFB, Polyclonal Antibody (Cat# AAA8001306)
Full Name
PDGFB Antibody: PerCP
Gene Names
PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~18.3 kDa BAD protein using Rabbit Anti-BAD Polyclonal Antibody (SPC-774). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-BAD Polyclonal Antibody (SPC-774) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~18.3 kDa. Other Band(s): ~25 kDa (PTM).)
BAD, Polyclonal Antibody (Cat# AAA8001365)
Full Name
BAD Antibody: ATTO 488
Gene Names
BAD; BBC2; BCL2L8
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~18.3 kDa BAD protein using Rabbit Anti-BAD Polyclonal Antibody (SPC-774). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-BAD Polyclonal Antibody (SPC-774) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~18.3 kDa. Other Band(s): ~25 kDa (PTM).)
BAD, Polyclonal Antibody (Cat# AAA8001372)
Full Name
BAD Antibody: Alkaline Phosphatase
Gene Names
BAD; BBC2; BCL2L8
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-FOXO1 Polyclonal Antibody . Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-FOXO1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Localization: Cytoplasm, Nucleus. Magnification: 10X. HRP-DAB Detection.)
FOXO1, Polyclonal Antibody (Cat# AAA8001655)
Full Name
FOXO1 Antibody: ATTO 594
Gene Names
FOXO1; FKH1; FKHR; FOXO1A
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 134 kDa EGF protein using Rabbit Anti-EGF Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-EGF Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 134 kDa. Other Band(s): 80 kDa, 55 kDa .)
EGF, Polyclonal Antibody (Cat# AAA8001706)
Full Name
EGF Antibody: ATTO 390
Gene Names
EGF; URG; HOMG4
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 52.9 kDa ATG4D protein using Rabbit Anti-ATG4D Polyclonal Antibody (SPC-635). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody (SPC-635) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 52.9 kDa. Other Band(s): ~45 and 48 kDa.)
ATG4D, Polyclonal Antibody (Cat# AAA809631)
Full Name
ATG4D Antibody: PerCP
Gene Names
ATG4D; APG4D; AUTL4; APG4-D
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse brain lysate showing detection of ~55.7 kDa PIK3CA protein using Rabbit Anti-PIK3CA Polyclonal Antibody (SPC-741). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3CA Polyclonal Antibody (SPC-741) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa.)
PIK3CA, Polyclonal Antibody (Cat# AAA8000782)
Full Name
PIK3CA Antibody: HRP
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of ~18.3 kDa BAD protein using Rabbit Anti-BAD Polyclonal Antibody (SPC-773). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-BAD Polyclonal Antibody (SPC-773) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~18.3 kDa. Other Band(s): 28 kDa (PTM).)
BAD, Polyclonal Antibody (Cat# AAA8001345)
Full Name
BAD Antibody
Gene Names
BAD; BBC2; BCL2L8
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~70 kDa HSP70 protein using Llama Anti-HSP70 Polyclonal Antibody (SPC-744). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Llama Anti-HSP70 Polyclonal Antibody (SPC-744) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Llama HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.)
HSP70, Polyclonal Antibody (Cat# AAA8000839)
Full Name
HSP70 Antibody: RPE
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
Reactivity
Human; Mouse; Rat
Applications
WB, EIA
Purity
Peptide Affinity Purified
Pricing
$495/0.1 mg | $1,995/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~27.3 kDa PDGFB protein using Rabbit Anti-PDGFB Polyclonal Antibody (SPC-770). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PDGFB Polyclonal Antibody (SPC-770) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~27.3 kDa.)
PDGFB, Polyclonal Antibody (Cat# AAA8001300)
Full Name
PDGFB Antibody: Alkaline Phosphatase
Gene Names
PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Pancreas cell lysates showing detection of 45.3 kDa ATG4A protein using Rabbit Anti-ATG4A Polyclonal Antibody (SPC-628). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody (SPC-628) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 45.3 kDa. Other Band(s): ~55-60 kDa.)
ATG4A, Polyclonal Antibody (Cat# AAA809528)
Full Name
ATG4A Antibody: ATTO 488
Gene Names
ATG4A; APG4A; AUTL2
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Citrulline, Monoclonal Antibody (Cat# AAA808522)
Full Name
Citrulline Antibody, Clone 6C2.1: ATTO 488
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,275/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 4F8. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Nitrotryptophan Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Nitrotryptophan, Monoclonal Antibody (Cat# AAA808859)
Full Name
Nitrotryptophan Antibody, Clone 4F8: PerCP
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,270/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 7D4. Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 uM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Dityrosine Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Dityrosine, Monoclonal Antibody (Cat# AAA808877)
Full Name
Dityrosine Antibody, Clone 7D4: PerCP
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,270/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody . Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
SFRP2, Polyclonal Antibody (Cat# AAA8001592)
Full Name
SFRP2 Antibody: HRP
Gene Names
SFRP2; FRP-2; SARP1; SDF-5
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-PDGFB Polyclonal Antibody . Tissue: Heart. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-PDGFB Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.)
PDGFB, Polyclonal Antibody (Cat# AAA8001700)
Full Name
PDGFB Antibody: HRP
Gene Names
PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~42.3 kDa Wnt5a protein using Rabbit Anti-Wnt5a Polyclonal Antibody (SPC-737). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Wnt5a Polyclonal Antibody (SPC-737) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~42.3 kDa. Other Band(s): ~35 kDa isoform 2.)
Wnt5a, Polyclonal Antibody (Cat# AAA8000713)
Full Name
Wnt5a Antibody: RPE
Gene Names
WNT5A; hWNT5A
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse brain lysate showing detection of ~67.5 kDa RYK protein using Rabbit Anti-RYK Polyclonal Antibody (SPC-740). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Rabbit Anti-RYK Polyclonal Antibody (SPC-740) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.5 kDa. Other Band(s): ~37 kDa degradation product.)
RYK, Polyclonal Antibody (Cat# AAA8000760)
Full Name
RYK Antibody: Alkaline Phosphatase
Gene Names
RYK; JTK5; RYK1; JTK5A; D3S3195
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of ~48.1 kDa SMAD3 protein using Rabbit Anti-SMAD3 Polyclonal Antibody (SPC-754). Lane 1: Molecular Weight Ladder (MW). Lane 2: A549. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD3 Polyclonal Antibody (SPC-754) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.1 kDa.)
SMAD3, Polyclonal Antibody (Cat# AAA8001019)
Full Name
SMAD3 Antibody: RPE
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
