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344 results for "stress-response-kinase" - showing 150-200
Protein rdoA (rdoA), Recombinant Protein (Cat# AAA1308820)
Full Name
Recombinant Salmonella typhi Protein rdoA (rdoA)
Gene Names
STY3884; yihE
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,825/1 mg (E-Coli) | $2,230/1 mg (Yeast) | $3,370/1 mg (Baculovirus) | $1,720/0.1 mg (Baculovirus) | $2,305/0.1 mg (Mammalian-Cell) | $1,020/0.1 mg (E-Coli) | $1,120/0.1 mg (Yeast) | $5,995/0.5 mg (Mammalian-Cell) | $1,195/0.02 mg (Baculovirus) | $1,460/0.02 mg (Mammalian-Cell) | $845/0.02 mg (E-Coli) | $985/0.02 mg (Yeast)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MAPK8 protein (Right) or empty vector (Left) detected with MAPK8 antibody)
MAPK8, Monoclonal Antibody (Cat# AAA837987)
Full Name
MAPK8 antibody
Applications
IF, WB
Purity
MAPK8 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
PRKAA2 (Thr172), Blocking Peptide (Cat# AAA9217924)
Full Name
PRKAA2 (Thr172) Antibody (Center) Blocking peptide
Gene Names
PRKAA2; AMPK; AMPK2; PRKAA; AMPKa2
STK25, cDNA Clone (Cat# AAA1269643)
Full Name
STK25 cDNA Clone
Gene Names
STK25; SOK1; YSK1
Pricing
$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using SEK1/MKK4 antibody)
SEK1, Polyclonal Antibody (Cat# AAA839763)
Full Name
SEK1 antibody
Gene Names
Map2k4; MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4
Applications
IF, IHC
Purity
SEK1 antibody was purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
Western Blot (WB)
(Western blot analysis of extracts from COLO cells and HUVEC cells, using MEKKK 4 antibody.)
MEKKK4, Polyclonal Antibody (Cat# AAA5301142)
Full Name
MEKKK4 antibody
Applications
WB, IF, EIA
Pricing
$405/0.1 mg | $1,805/5x0.1 mg
Western Blot (WB)
(Mouse Stk25 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Stk25 antibody detected the Stk25 protein (arrow).)
Stk25, Polyclonal Antibody (Cat# AAA9210519)
Full Name
Mouse Stk25 Antibody (C-term)
Gene Names
Stk25; Ysk1; C86992; AU018434; Ste20-like; 1500019J11Rik
Reactivity
Human
Applications
WB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
YSK, Blocking Peptide (Cat# AAA9227406)
Full Name
YSK Antibody (C-term) Blocking Peptide
Gene Names
STK25; SOK1; YSK1
Protein rdoA (rdoA), Recombinant Protein (Cat# AAA1452691)
Full Name
Recombinant Salmonella typhimurium Protein rdoA (rdoA)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,825/1 mg (E-Coli) | $2,230/1 mg (Yeast) | $3,370/1 mg (Baculovirus) | $1,720/0.1 mg (Baculovirus) | $2,305/0.1 mg (Mammalian-Cell) | $1,020/0.1 mg (E-Coli) | $1,120/0.1 mg (Yeast) | $5,995/0.5 mg (Mammalian-Cell) | $1,195/0.02 mg (Baculovirus) | $1,460/0.02 mg (Mammalian-Cell) | $845/0.02 mg (E-Coli) | $985/0.02 mg (Yeast)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SEK1/MKK4 antibody (left) or the same antibody preincubated with with blocking peptide (right))
SEK1, Polyclonal Antibody (Cat# AAA5301946)
Full Name
SEK1 antibody
Gene Names
Map2k4; MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4
Applications
IHC, WB
Purity
SEK1 antibody was purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
MAP3K5, Polyclonal Antibody (Cat# AAA1489237)
Full Name
Rabbit Anti Human MAP3K5 PolyClonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$325/0.1 mL | $1,465/5x0.1 mL
JLP (SPAG9), Blocking Peptide (Cat# AAA9226758)
Full Name
JLP (SPAG9) Antibody (Center) Blocking peptide
Gene Names
SPAG9; JLP; CT89; HLC4; HLC6; JIP4; PHET; PIG6; HLC-6; JIP-4
ERN1 / Serine/Threonine-Protein Kinase/Endoribonuclease IRE1, Recombinant Protein (Cat# AAA2903499)
Full Name
Human ERN1 / Serine/Threonine-Protein Kinase/Endoribonuclease IRE1 Recombinant Protein
Gene Names
ERN1; IRE1; IRE1P; IRE1a; hIRE1p
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
JLP (SPAG9), Blocking Peptide (Cat# AAA9226757)
Full Name
JLP (SPAG9) Antibody (N-term) Blocking peptide
Gene Names
SPAG9; JLP; CT89; HLC4; HLC6; JIP4; PHET; PIG6; HLC-6; JIP-4
HIPK1, Blocking Peptide (Cat# AAA9219152)
Full Name
HIPK1 Antibody (C-term) Blocking peptide
Gene Names
HIPK1; Myak; Nbak2
Cdk9, Blocking Peptide (Cat# AAA9221482)
Full Name
Mouse Cdk9 Antibody (C-term) Blocking Peptide
Gene Names
Cdk9; PITALRE
CDK5, Blocking Peptide (Cat# AAA9229690)
Full Name
CDK5 Blocking Peptide (C-term)
Gene Names
CDK5; LIS7; PSSALRE
PCM-1, Blocking Peptide (Cat# AAA9226935)
Full Name
PCM-1 Antibody (C-term) Blocking Peptide
Gene Names
PCM1; PTC4; RET/PCM-1
Protein rdoA (rdoA), Recombinant Protein (Cat# AAA1405598)
Full Name
Recombinant Shigella flexneri Protein rdoA (rdoA)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,825/1 mg (E-Coli) | $2,230/1 mg (Yeast) | $3,370/1 mg (Baculovirus) | $1,720/0.1 mg (Baculovirus) | $2,305/0.1 mg (Mammalian-Cell) | $1,020/0.1 mg (E-Coli) | $1,120/0.1 mg (Yeast) | $5,995/0.5 mg (Mammalian-Cell) | $1,195/0.02 mg (Baculovirus) | $1,460/0.02 mg (Mammalian-Cell) | $845/0.02 mg (E-Coli) | $985/0.02 mg (Yeast)
Stk25, Blocking Peptide (Cat# AAA9220810)
Full Name
Mouse Stk25 Antibody (C-term) Blocking Peptide
Gene Names
Stk25; Ysk1; C86992; AU018434; Ste20-like; 1500019J11Rik
CDK5, Blocking Peptide (Cat# AAA9229498)
Full Name
CDK5 Blocking Peptide (C-term)
Gene Names
CDK5; LIS7; PSSALRE
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MAPK8 protein (Right) or empty vector (Left) detected with MAPK8 antibody)
MAPK8, Monoclonal Antibody (Cat# AAA838041)
Full Name
MAPK8 antibody
Applications
WB
Purity
MAPK8 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
RSK3, Recombinant Protein (Cat# AAA5304588)
Full Name
RSK3 protein
Purity
RSK3 protein was purified using a a proprietary chromatographic technique.
Pricing
$555/0.005 mg | $2,490/5x0.005 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of MAPK8 protein in paraffin embedded Carcinoma of Human prostate tissue using MAPK8 antibody)
MAPK8, Monoclonal Antibody (Cat# AAA838512)
Full Name
MAPK8 antibody
Applications
IHC, WB
Purity
MAPK8 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
STK29, Blocking Peptide (Cat# AAA9226657)
Full Name
STK29 Antibody (C-term) Blocking Peptide
Gene Names
BRSK2; SAD1; SADA; STK29; PEN11B; C11orf7
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NEK11 protein using NEK11 antibody)
NEK11, Monoclonal Antibody (Cat# AAA837914)
Full Name
NEK11 antibody
Applications
IF, WB
Purity
NEK11 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CDK9 expression in HeLa cell lysate (MBS179457).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK9 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDK9 )
CDK9, Monoclonal Antibody (Cat# AAA179457)
Full Name
Anti-CDK9 Rabbit Monoclonal Antibody
Gene Names
CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
Reactivity
Human
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
PRKAA1/2, Blocking Peptide (Cat# AAA9229504)
Full Name
PRKAA1/2 Blocking Peptide (N-term)
Gene Names
PRKAA1; AMPK; AMPKa1
Protein rdoA (rdoA), Recombinant Protein (Cat# AAA1417996)
Full Name
Recombinant Salmonella paratyphi A Protein rdoA (rdoA)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,825/1 mg (E-Coli) | $2,230/1 mg (Yeast) | $3,370/1 mg (Baculovirus) | $1,720/0.1 mg (Baculovirus) | $2,305/0.1 mg (Mammalian-Cell) | $1,020/0.1 mg (E-Coli) | $1,120/0.1 mg (Yeast) | $5,995/0.5 mg (Mammalian-Cell) | $1,195/0.02 mg (Baculovirus) | $1,460/0.02 mg (Mammalian-Cell) | $845/0.02 mg (E-Coli) | $985/0.02 mg (Yeast)
Serine/threonine-protein kinase 25, Recombinant Protein (Cat# AAA9420708)
Full Name
Recombinant Human Serine/threonine-protein kinase 25
Gene Names
STK25; SOK1; YSK1
Reactivity
Human
Purity
Greater than 90% as determined by SDS-PAGE.
Pricing
$285/0.02 mg | $470/0.1 mg | $1,785/1 mg | $7,885/5x1 mg
Western Blot (WB)
(Western blot analysis of IKK gamma expression in Jurkat cell lysate (MBS179480).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKBKG monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IKBKG )
IKK gamma, Monoclonal Antibody (Cat# AAA179480)
Full Name
Anti-IKK gamma Rabbit Monoclonal Antibody
Gene Names
IKBKG; IP; IP1; IP2; FIP3; IKKG; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; IKKAP1; ZC2HC9; IKK-gamma
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
MLCK, Blocking Peptide (Cat# AAA9227420)
Full Name
MLCK Antibody (N-term) Blocking peptide
Gene Names
MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
Western Blot (WB)
(All lanes : Anti-MSK1 Antibody at 1:2000 dilutionLane 1: 293 whole cell lysateLane 2: HT-29 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 90 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
MSK1, Polyclonal Antibody (Cat# AAA9231029)
Full Name
MSK1 (Ser360) Antibody
Gene Names
RPS6KA5; MSK1; RLPK; MSPK1
Reactivity
Human
Predicted: Chicken, Mouse
Predicted: Chicken, Mouse
Applications
WB, EIA
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
STK25, Blocking Peptide (Cat# AAA9228987)
Full Name
STK25 Blocking Peptide (C-term)
Gene Names
STK25; SOK1; YSK1
Western Blot (WB)
(Western blot analysis of Phospho-AMPK alpha (T172) expression in Mouse heart lysate (MBS1750261).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAA2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRKAA2 )
AMPK alpha, Monoclonal Antibody (Cat# AAA1750261)
Full Name
Anti-Phospho-AMPK alpha (T172) Rabbit Monoclonal Antibody
Gene Names
PRKAA2; AMPK; AMPK2; PRKAA; AMPKa2
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
RPS6KA5, Blocking Peptide (Cat# AAA9229804)
Full Name
RPS6KA5 Blocking Peptide (C-term)
Gene Names
RPS6KA5; MSK1; RLPK; MSPK1
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using SEK1/MKK4 (Phospho-Thr261) antibody)
SEK1, Polyclonal Antibody (Cat# AAA5302524)
Full Name
SEK1 antibody
Gene Names
Map2k4; MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4
Applications
IF, IHC
Purity
SEK1 antibody was purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
STK25, siRNA (Cat# AAA8237142)
Full Name
STK25 siRNA (Mouse)
Gene Names
Stk25; Ysk1; C86992; AU018434; Ste20-like; 1500019J11Rik
Reactivity
Mouse
Applications
RNAi
Purity
> 97%
Pricing
$395/15 nmol | $555/30 nmol | $2,440/5x30 nmol
Western Blot (WB)
(Western blot analysis of lysates from 293 cell line, untreated or treated with Calyculin A, 100nM, 30min, using Phospho-MSK1 (Thr581) Antibody (upper) or Beta-actin (lower).)
MSK1, Polyclonal Antibody (Cat# AAA9231101)
Full Name
Phospho-MSK1 (Thr581) Antibody
Gene Names
RPS6KA5; MSK1; RLPK; MSPK1
Reactivity
Human
Predicted: Chicken, Mouse
Predicted: Chicken, Mouse
Applications
WB, EIA
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Western Blot (WB)
(All lanes : Anti-STK25 Antibody at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Human testis lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
STK25, Polyclonal Antibody (Cat# AAA9231096)
Full Name
STK25 Antibody
Gene Names
STK25; SOK1; YSK1
Reactivity
Human
Applications
WB, EIA
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3-Src, using Phospho-P85 alpha(Tyr508) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
PI3K p85 alpha, Polyclonal Antibody (Cat# AAA9613173)
Full Name
Phospho-PI3K p85 alpha (Tyr508) Antibody
Gene Names
PIK3R1; p85; AGM7; GRB1; p85-ALPHA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (83%)
Predicted Reactivity: Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (83%)
Applications
WB, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB)
(Western blot analysis of CDK5 expression in HeLa cell lysate (MBS179347).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDK5 )
CDK5, Monoclonal Antibody (Cat# AAA179347)
Full Name
Anti-CDK5 Rabbit Monoclonal Antibody
Gene Names
CDK5; LIS7; PSSALRE
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-MSK1 (S376) expression in HEK293 cell lysate treated with EGF (MBS1750284).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KA5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPS6KA5 )
MSK1, Monoclonal Antibody (Cat# AAA1750284)
Full Name
Anti-Phospho-MSK1 (S376) Rabbit Monoclonal Antibody
Gene Names
RPS6KA5; MSK1; RLPK; MSPK1
Reactivity
Human, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$310/0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney, using AMPK alpha 1 Antibody(MBS179515)PRKAA1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PRKAA1 Antibody (MBS179515)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
AMPK alpha 1, Monoclonal Antibody (Cat# AAA179515)
Full Name
Anti-AMPK alpha 1 Rabbit Monoclonal Antibody
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
STK25, siRNA (Cat# AAA8209321)
Full Name
STK25 siRNA (Human)
Gene Names
STK25; SOK1; YSK1
Reactivity
Human
Applications
RNAi
Purity
> 97%
Pricing
$395/15 nmol | $555/30 nmol | $2,440/5x30 nmol
Western Blot (WB)
(Western blot analysis of Phospho-AMPK alpha 1 (S496) expression in (1) 293T cell lysate treated with LP; (2) 293T cell lysate (MBS1750246).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRKAA1 )
AMPK alpha 1, Monoclonal Antibody (Cat# AAA1750246)
Full Name
Anti-Phospho-AMPK alpha 1 (S496) Rabbit Monoclonal Antibody
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human
Applications
IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon, using Myosin light chain kinase Antibody(MBS179722)MYLK was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYLK Antibody (MBS179722)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Myosin light chain kinase, Monoclonal Antibody (Cat# AAA179722)
Full Name
Anti-Myosin light chain kinase Rabbit Monoclonal Antibody
Gene Names
MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
CDK5, Blocking Peptide (Cat# AAA9229541)
Full Name
CDK5 Blocking Peptide (C-term)
Gene Names
CDK5; LIS7; PSSALRE
Western Blot (WB)
(Western blot analysis of extracts from Colo205, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody. The lane on the left was treated with blocking peptide.)
p38 MAPK, Polyclonal Antibody (Cat# AAA9613047)
Full Name
Phospho-p38 MAPK (Thr180/Tyr182) Antibody
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (91%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (91%)
Applications
WB, IHC, IF, ICC, IP, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$305/0.1 mL | $365/0.2 mL | $1,495/5x0.2 mL
CDK2 / Cyclin-Dependent Kinase 2, Recombinant Protein (Cat# AAA2903049)
Full Name
Human CDK2 / Cyclin-Dependent Kinase 2 Recombinant Protein
Gene Names
CDK2; CDKN2; p33(CDK2)
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
