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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
3255 results for "Primary Antibodies Detection antibody" - showing 1250-1300
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
O-GlcNAc, Monoclonal Antibody (Cat# AAA808539)
Full Name
O-GlcNAc Antibody, Clone 9H6: ATTO 390
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
PEG Purified
Pricing
$560/0.1 mg | $2,280/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (SPC-713). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (SPC-713) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Multi-Pass Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Calcium Sensing Receptor Antibody (D) Composite.)
Calcium Sensing Receptor, Polyclonal Antibody (Cat# AAA8000350)
Full Name
Calcium Sensing Receptor Antibody: HRP
Gene Names
CASR; CAR; FHH; FIH; HHC; EIG8; HHC1; NSHPT; PCAR1; hCasR; GPRC2A; HYPOC1
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 150.5 kDa PALLD protein using Rabbit Anti-PALLD Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-PALLD Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 150.5 kDa. Other Band(s): 86 kDa.)
Palladin, Polyclonal Antibody (Cat# AAA8001750)
Full Name
Palladin Antibody: Alkaline Phosphatase
Gene Names
PALLD; MYN; PNCA1; CGI151; SIH002; CGI-151
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ESR1 Polyclonal Antibody (SPC-708). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ESR1 Polyclonal Antibody (SPC-708) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ESR1 Antibody. (D) Composite.)
ESR1, Polyclonal Antibody (Cat# AAA8000285)
Full Name
ESR1 Antibody: ATTO 488
Gene Names
ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Schistosoma japonicum Purified GST showing detection of 26 kDa GST protein using Mouse Anti-GST Monoclonal Antibody, Clone 3E2. Lane 1: Molecular Weight Ladder (MW). Lane 2: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein. Lane 3: GST. Load: 1 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-GST Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 26 kDa.)
GST, Monoclonal Antibody (Cat# AAA809273)
Full Name
GST Antibody, Clone 3E2: PerCP
Reactivity
Species Independent
Applications
WB, EIA
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8. Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.)
ENaC beta, Monoclonal Antibody (Cat# AAA808298)
Full Name
ENaC beta Antibody, Clone 7B8: FITC
Gene Names
Scnn1b; RNENACB
Reactivity
Mouse
Applications
WB, IHC
Purity
Protein G Purified
Pricing
$470/0.1 mg | $1,900/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Trypanosoma brucei brucei cells showing detection of ~71 kDa TbHsp70 protein using Rabbit Anti-TbHsp70 Polyclonal Antibody (SPC-747). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cells incubated for 1 hour at 37 degree C. Lane 3: Cells incubated for 1 hour at 42 degree C. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TbHsp70 Polyclonal Antibody (SPC-747) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71 kDa.)
TbHsp70, Polyclonal Antibody (Cat# AAA8000889)
Full Name
TbHsp70 Antibody: FITC
Reactivity
Trypanosoma brucei brucei
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 1F2. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
KDEL, Monoclonal Antibody (Cat# AAA809076)
Full Name
KDEL Antibody, Clone 1F2: RPE
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Protein G Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Lung showing detection of ~93.5 kDa Axin-2 protein using Rabbit Anti-Axin-2 Polyclonal Antibody (SPC-750). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Lung. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Axin-2 Polyclonal Antibody (SPC-750) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~93.5 kDa.)
Axin-2, Polyclonal Antibody (Cat# AAA8000946)
Full Name
Axin-2 Antibody: PerCP
Gene Names
AXIN2; AXIL; ODCRCS
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 11G1 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 2 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 75kDa RDHKH-BSA, and REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808424)
Full Name
N-terminal Arginylation Antibody, Clone 11G1: FITC
Reactivity
Species Independent
Applications
WB
Purity
Protein G Purified
Pricing
$570/0.1 mg | $2,340/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 142.5 kDa AMBRA1 protein using Rabbit Anti-AMBRA1 Polyclonal Antibody (SPC-644). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AMBRA1 Polyclonal Antibody (SPC-644) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 142.5 kDa. Other Band(s): 50 kDa.)
AMBRA1, Polyclonal Antibody (Cat# AAA809746)
Full Name
AMBRA1 Antibody: ATTO 594
Gene Names
AMBRA1; DCAF3; WDR94
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human HeLa showing detection of 90 kda Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone M10E3R . Lane 1: Molecular Weight Ladder (MW). Lane 2: Hela (10ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 90 kda. Other Band(s): .)
Hsp90 alpha, Monoclonal Antibody (Cat# AAA809364)
Full Name
Hsp90 alpha Antibody, Clone M10E3R: RPE
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
Reactivity
Human
Applications
WB, EIA
Purity
Protein A Purified
Pricing
$500/0.1 mg | $2,020/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~63 kDa Frizzled 7 protein using Rabbit Anti-Frizzled 7 Polyclonal Antibody (SPC-761). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Frizzled 7 Polyclonal Antibody (SPC-761) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~63 kDa.)
Frizzled 7, Polyclonal Antibody (Cat# AAA8001129)
Full Name
Frizzled 7 Antibody
Gene Names
FZD7; FzE3
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~35.7 kDa c-Jun protein using Rabbit Anti-c-Jun Polyclonal Antibody (SPC-728). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Jun Polyclonal Antibody (SPC-728) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~35.7 kDa. Other Band(s): ~25 kDa potential degradation product.)
c-Jun, Polyclonal Antibody (Cat# AAA8000550)
Full Name
c-Jun Antibody: PerCP
Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808380)
Full Name
N-terminal Arginylation Antibody, Clone 4A9: ATTO 594
Reactivity
Species Independent
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$580/0.1 mg | $2,385/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IGF-1 Polyclonal Antibody (SPC-699). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IGF-1 Polyclonal Antibody (SPC-699) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IGF-1 Antibody. (D) Composite.)
IGF-1, Polyclonal Antibody (Cat# AAA8000204)
Full Name
IGF-1 Antibody: Biotin
Gene Names
IGF1; MGF; IGFI; IGF-I
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 94 kDa GRP94 protein using Mouse Anti-GRP94 Monoclonal Antibody, Clone 6B12. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-GRP94 Monoclonal Antibody at 1:1000 for O/N at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 94 kDa.)
GRP94, Monoclonal Antibody (Cat# AAA809114)
Full Name
GRP94 Antibody, Clone 6B12
Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Protein G Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~46.7 kDa GSK3 beta protein using Rabbit Anti-GSK3 beta Polyclonal Antibody (SPC-758). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain . Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GSK3 beta Polyclonal Antibody (SPC-758) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~46.7 kDa.)
GSK3 beta, Polyclonal Antibody (Cat# AAA8001079)
Full Name
GSK3 beta Antibody: ATTO 594
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG3 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG3 Antibody. (D) Composite.)
ATG3, Polyclonal Antibody (Cat# AAA809953)
Full Name
ATG3 Antibody: HRP
Gene Names
ATG3; APG3; APG3L; PC3-96; APG3-LIKE
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Kidney, Liver showing detection of 47.2 kDa PTEN protein using Rabbit Anti-PTEN Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Lane 3: Rat Liver. Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-PTEN Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 47.2 kDa. Other Band(s): 64 kDa.)
PTEN, Polyclonal Antibody (Cat# AAA8001624)
Full Name
PTEN Antibody: Alkaline Phosphatase
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 150.5 kDa PALLD protein using Rabbit Anti-PALLD Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-PALLD Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 150.5 kDa. Other Band(s): 86 kDa.)
Palladin, Polyclonal Antibody (Cat# AAA8001752)
Full Name
Palladin Antibody: Biotin
Gene Names
PALLD; MYN; PNCA1; CGI151; SIH002; CGI-151
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Bovine serum albumin showing detection of Methylated Lysine protein using Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158). Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158) at 1:1000. Methylated Lysine in BSA (Left) and Methylated BSA (Right).)
Methylated Lysine, Polyclonal Antibody (Cat# AAA809375)
Full Name
Methylated Lysine Antibody: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, IP, EIA
Purity
Protein A Purified
Pricing
$485/0.1 mg | $1,950/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Trypanosoma brucei brucei cells showing detection of ~71 kDa TbHsp70 protein using Rabbit Anti-TbHsp70 Polyclonal Antibody (SPC-747). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cells incubated for 1 hour at 37 degree C. Lane 3: Cells incubated for 1 hour at 42 degree C. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TbHsp70 Polyclonal Antibody (SPC-747) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71 kDa.)
TbHsp70, Polyclonal Antibody (Cat# AAA8000878)
Full Name
TbHsp70 Antibody: ATTO 390
Reactivity
Trypanosoma brucei brucei
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8. Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.)
ENaC beta, Monoclonal Antibody (Cat# AAA808295)
Full Name
ENaC beta Antibody, Clone 7B8: Alkaline Phosphatase
Gene Names
Scnn1b; RNENACB
Reactivity
Mouse
Applications
WB, IHC
Purity
Protein G Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged showing detection of 36 kDa Metallothionein protein using Mouse Anti-Metallothionein Monoclonal Antibody, Clone 1F5. Lane 1: Molecular Weight Ladder (MW). Lane 2: GST. Lane 3: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged. Load: 1 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Metallothionein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 36 kDa.)
Metallothionein, Monoclonal Antibody (Cat# AAA809306)
Full Name
Metallothionein Antibody, Clone 1F5: FITC
Reactivity
Bacteria (Pseudomonas aeruginosa)
Applications
WB, EIA
Purity
Protein G Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4D12 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 2 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 75kDa, 20kDa RDHKH-BSA; 250kDa, 75kDa REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808398)
Full Name
N-terminal Arginylation Antibody, Clone 4D12: ATTO 594
Reactivity
Species Independent
Applications
WB
Purity
Protein G Purified
Pricing
$580/0.1 mg | $2,385/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 142.5 kDa AMBRA1 protein using Rabbit Anti-AMBRA1 Polyclonal Antibody (SPC-644). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AMBRA1 Polyclonal Antibody (SPC-644) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 142.5 kDa. Other Band(s): 50 kDa.)
AMBRA1, Polyclonal Antibody (Cat# AAA809743)
Full Name
AMBRA1 Antibody: ATTO 390
Gene Names
AMBRA1; DCAF3; WDR94
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 11G1 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 2 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 75kDa RDHKH-BSA, and REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808427)
Full Name
N-terminal Arginylation Antibody, Clone 11G1: PerCP
Reactivity
Species Independent
Applications
WB
Purity
Protein G Purified
Pricing
$580/0.1 mg | $2,380/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 395.2 kDa WDFY3 protein using Rabbit Anti-WDFY3 Polyclonal Antibody (SPC-666). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody (SPC-666) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 395.2 kDa.)
WDFY3, Polyclonal Antibody (Cat# AAA809895)
Full Name
WDFY3 Antibody: Alkaline Phosphatase
Gene Names
WDFY3; ALFY; BCHS; MCPH18; ZFYVE25
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of ~95 kDa Axin-1 protein using Rabbit Anti-Axin-1 Polyclonal Antibody (SPC-748). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Axin-1 Polyclonal Antibody (SPC-748) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~95 kDa.)
Axin-1, Polyclonal Antibody (Cat# AAA8000895)
Full Name
Axin-1 Antibody
Gene Names
AXIN1; AXIN; PPP1R49
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.)
ATG9B, Polyclonal Antibody (Cat# AAA809764)
Full Name
ATG9B Antibody: ATTO 594
Gene Names
ATG9B; SONE; APG9L2; NOS3AS
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.)
ATG9B, Polyclonal Antibody (Cat# AAA809771)
Full Name
ATG9B Antibody: Biotin
Gene Names
ATG9B; SONE; APG9L2; NOS3AS
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 395.2 kDa WDFY3 protein using Rabbit Anti-WDFY3 Polyclonal Antibody (SPC-666). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody (SPC-666) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 395.2 kDa.)
WDFY3, Polyclonal Antibody (Cat# AAA809897)
Full Name
WDFY3 Antibody: Biotin
Gene Names
WDFY3; ALFY; BCHS; MCPH18; ZFYVE25
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~63 kDa Frizzled 7 protein using Rabbit Anti-Frizzled 7 Polyclonal Antibody (SPC-761). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Frizzled 7 Polyclonal Antibody (SPC-761) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~63 kDa.)
Frizzled 7, Polyclonal Antibody (Cat# AAA8001142)
Full Name
Frizzled 7 Antibody: HRP
Gene Names
FZD7; FzE3
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of ~18.3 kDa BAD protein using Rabbit Anti-BAD Polyclonal Antibody (SPC-773). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-BAD Polyclonal Antibody (SPC-773) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~18.3 kDa. Other Band(s): 28 kDa (PTM).)
BAD, Polyclonal Antibody (Cat# AAA8001347)
Full Name
BAD Antibody: ATTO 488
Gene Names
BAD; BBC2; BCL2L8
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 45.3 kda ATG4A protein using Rabbit Anti-ATG4A Polyclonal Antibody (SPC-629). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody (SPC-629) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 45.3 kda.)
ATG4A, Polyclonal Antibody (Cat# AAA809557)
Full Name
ATG4A Antibody: HRP
Gene Names
ATG4A; APG4A; AUTL2
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~55 kDa AKT3 protein using Rabbit Anti-AKT3 Polyclonal Antibody (SPC-769). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AKT3 Polyclonal Antibody (SPC-769) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55 kDa.)
AKT3, Polyclonal Antibody (Cat# AAA8001284)
Full Name
AKT3 Antibody: Biotin
Gene Names
AKT3; MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 1B8 . Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell membrane, Endoplasmic Reticulum, Golgi apparatus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) CD74 Antibody (D) Composite.)
CD74, Monoclonal Antibody (Cat# AAA808463)
Full Name
CD74 Antibody, Clone 1B8: PerCP
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Applications
WB, ICC, IF
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody, Clone 12F7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxynonenal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 4-Hydroxynonenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
4-Hydroxynonenal, Monoclonal Antibody (Cat# AAA808702)
Full Name
4-Hydroxynonenal Antibody, Clone 12F7: ATTO 488
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,275/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~100 kDa LGR5 protein using Rabbit Anti-LGR5 Polyclonal Antibody (SPC-764). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-LGR5 Polyclonal Antibody (SPC-764) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~100 kDa. Other Band(s): 97 kDa, 70 kDa.)
LGR5, Polyclonal Antibody (Cat# AAA8001194)
Full Name
LGR5 Antibody: Biotin
Gene Names
LGR5; FEX; HG38; GPR49; GPR67; GRP49
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~134.3 kDa EGFR protein using Rabbit Anti-EGFR Polyclonal Antibody (SPC-771). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-EGFR Polyclonal Antibody (SPC-771) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~134.3 kDa. Other Band(s): 44 kDa and 69 kDa.)
EGFR, Polyclonal Antibody (Cat# AAA8001321)
Full Name
EGFR Antibody: FITC
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human Serum starved HeLa cells treated with PMA showing detection of 41 kDa c-fos protein using Mouse Anti-c-fos Monoclonal Antibody, Clone 5B10. Lane 1: Molecular Weight Ladder (MW). Lane 2: Serum starved HeLa cell lysates. Lane 3: Serum starved HeLa cells treated with PMA (Phorbol 12-myristate 13-acetate). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-c-fos Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 41 kDa. Other Band(s): 62 kDa. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.)
c-Fos, Monoclonal Antibody (Cat# AAA809170)
Full Name
c-Fos Antibody, Clone 5B10: ATTO 488
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Human
Applications
WB
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human Serum starved HeLa cells treated with PMA showing detection of 41 kDa c-fos protein using Mouse Anti-c-fos Monoclonal Antibody, Clone 5B11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Serum starved HeLa cell lysates. Lane 3: Serum starved HeLa cells treated with PMA (Phorbol 12-myristate 13-acetate). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-c-fos Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 41 kDa. Other Band(s): 62 kDa. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.)
c-Fos, Monoclonal Antibody (Cat# AAA809195)
Full Name
c-Fos Antibody, Clone 5B11: Alkaline Phosphatase
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Human
Applications
WB
Purity
Protein G Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody (SPC-743). Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 uM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody (SPC-743) at 1:60 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) HSP70 Antibody (Acetyl Lys77). (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
HSP70, Polyclonal Antibody (Cat# AAA8000821)
Full Name
HSP70 Antibody (Acetyl Lys77): RPE
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
Reactivity
Human; Mouse; Rat
Applications
WB, EIA, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$555/0.1 mL | $2,260/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4. Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.)
ENaC alpha, Monoclonal Antibody (Cat# AAA808284)
Full Name
ENaC alpha Antibody, Clone 2G4: RPE
Gene Names
Scnn1a; ENaCA
Reactivity
Mouse; Rat
Applications
WB, IHC
Purity
Protein G Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~12.6 kDa 4EBP1 protein using Rabbit Anti-4EBP1 Polyclonal Antibody (SPC-781). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-4EBP1 Polyclonal Antibody (SPC-781) at 1:500 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~12.6 kDa.)
4EBP1, Polyclonal Antibody (Cat# AAA8001490)
Full Name
4EBP1 Antibody: ATTO 390
Gene Names
EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 153.1 kDa PIK3R4 protein using Rabbit Anti-PIK3R4 Polyclonal Antibody (SPC-660). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3R4 Polyclonal Antibody (SPC-660) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 153.1 kDa.)
PIK3R4, Polyclonal Antibody (Cat# AAA809850)
Full Name
PIK3R4 Antibody
Gene Names
PIK3R4; p150; VPS15
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Pseudomonas aeruginosa Purified protein showing detection of ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST) Metallothionein protein using Rabbit Anti-Metallothionein Polyclonal Antibody (SPC-717). Lane 1: Molecular Weight Ladder (MW). Lane 2: Blank. Lane 3: Pseudomonas aeruginosa purified protein with GST tag. Load: 25 ug. Block: 1% milk, 2% BSA. Primary Antibody: Rabbit Anti-Metallothionein Polyclonal Antibody (SPC-717) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Mouse Anti-Rabbit IgG: AP at 1:1000 for 60 min at RT. Color Development: Alkaline phosphate activity colorimetric assay. Predicted/Observed Size: ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST). Courtesy of: Michael Lynes and Amy Thees, University of Connecticut.)
Metallothionein, Polyclonal Antibody (Cat# AAA8000356)
Full Name
Metallothionein Antibody: ATTO 390
Reactivity
Bacteria (Pseudomonas aeruginosa)
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4D12 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 2 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 75kDa, 20kDa RDHKH-BSA; 250kDa, 75kDa REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808394)
Full Name
N-terminal Arginylation Antibody, Clone 4D12
Reactivity
Species Independent
Applications
WB
Purity
Protein G Purified
Pricing
$535/0.1 mg | $2,160/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 11G1 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 2 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 75kDa RDHKH-BSA, and REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808413)
Full Name
N-terminal Arginylation Antibody, Clone 11G1: ATTO 390
Reactivity
Species Independent
Applications
WB
Purity
Protein G Purified
Pricing
$585/0.1 mg | $2,385/5x0.1 mg
