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7565 results for "IgG h l Secondary Antibodies" - showing 50-100

Testing Data (Overlay histogram showing Ramos cells stained with MBS9203899 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9203899, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

BACH2, Polyclonal Antibody (Cat# AAA9203899)

Full Name
BACH2 Antibody (C-term)
Gene Names
BACH2; BTBD25
Reactivity
Human
Applications
FC, WB, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemical (IHC) (Immunohistochemical analysis ofparaffin-embedded human kidney tissue using MBS9202710 performed on the Leica® BOND RXm.Samples were incubated with primary antibody(1/500) for 1 hours at room temperature.A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

ALPL, Polyclonal Antibody (Cat# AAA9202710)

Full Name
ALPL Antibody (Center)
Gene Names
ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
Reactivity
Human, mouse
Applications
FC, EIA, IHC-P-Leica, WB, IF
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 μg of lysates per lane.Antibodies: TIM-1 3809 (8 μg/mL), overnight incubation at 4˚ C in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

TIM-1, Polyclonal Antibody (Cat# AAA150916)

Full Name
TIM-1 Antibody
Gene Names
HAVCR1; TIM; KIM1; TIM1; HAVCR; KIM-1; TIM-1; TIMD1; TIMD-1; HAVCR-1
Reactivity
Human, Mouse
Applications
EIA, WB, IHC, IF
Purity
TIM-1 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using PMS1 antibody (MBS9134056) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ( MBS128200 ) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

PMS1, Polyclonal Antibody (Cat# AAA9134056)

Full Name
PMS1 Polyclonal Antibody
Gene Names
PMS1; MLH2; PMSL1; hPMS1; HNPCC3
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Flow Cytometry (FC/FACS) (Flow cytometric analysis of A549 cells using CF150 Antibody (Center) (green) compared to an isotype control of rabbit IgG(blue). MBS9214999 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

CF150, Polyclonal Antibody (Cat# AAA9214999)

Full Name
CF150 Antibody (Center)
Gene Names
MB21D1; cGAS; h-cGAS; C6orf150
Reactivity
Human
Applications
FC/FACS, EIA, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (All lanes : Anti-GAPDH Antibody at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: C6 whole cell lysatesLane 3: Hela whole cell lysatesLane 4: HUVEC whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

GAPDH, Monoclonal Antibody (Cat# AAA9200139)

Full Name
GAPDH Antibody
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human, mouse, rat
Applications
WB, EIA, IF, IHC
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$435/0.4 mL | $190/0.08 mL | $1,705/5x0.4 mL
Western Blot (WB) (Anti-SLC5A12 Antibody (C-term) at 1:2000 dilution + Y79 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

SLC5A12, Polyclonal Antibody (Cat# AAA9217008)

Full Name
SLC5A12 Antibody (C-term)
Gene Names
SLC5A12; SMCT2
Reactivity
Human
Applications
WB, IHC-P, FC, EIA
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (Anti-SRC Antibody at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size :60 kDa Blocking/Dilution buffer:5% NFDM/TBST.)

SRC, Monoclonal Antibody (Cat# AAA9200273)

Full Name
SRC Antibody
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human, mouse
Applications
WB, EIA, IF
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$435/0.4 mL | $190/0.08 mL | $1,705/5x0.4 mL
Immunohistochemistry IHC (MBS9207973 staining HHLA2 in human pancreas tissue sections by Immunohistochemistry(IHC-P - paraformaldehyde-fixed,paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSAfor 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citratebuffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. Aundiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

HHLA2, Polyclonal Antibody (Cat# AAA9207973)

Full Name
HHLA2 Antibody (N-term)
Gene Names
HHLA2; B7H7; B7-H7
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Anti-TFE3 Antibody (N-term)at 1:2000 dilution + HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

TFE3, Polyclonal Antibody (Cat# AAA9211960)

Full Name
TFE3 Antibody (N-term)
Gene Names
TFE3; TFEA; RCCP2; RCCX1; bHLHe33
Reactivity
Human, mouse
Applications
WB, EIA, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data TD ( All lanes : Anti-SQSTM1 (p62) Antibody (C-term) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: THP-1 whole cell lysate Lane 5: PANC-1 whole cell lysate Lane 6: A20 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

SQSTM1 (p62), Polyclonal Antibody (Cat# AAA9204361)

Full Name
SQSTM1 (p62) Antibody (C-term)
Gene Names
SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B; FTDALS3
Reactivity
Human,Mouse,Rat

Predicted: Rat
Applications
WB, IHC-P , IF, E
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot analysis of lysates from SW620, PC-3 cell line, mouse kidney, rat kidney tissue lysate (from left to right), using SUPV3L1 Antibody (N-term). MBS9203101 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

SUPV3L1, Polyclonal Antibody (Cat# AAA9203101)

Full Name
SUPV3L1 Antibody (N-term)
Gene Names
SUPV3L1; SUV3
Reactivity
Human, mouse, rat
Applications
WB, EIA, IHC-P, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Anti-MARCO Antibody (N-term) at 1:500 dilution + HACAT whole cell lysateLysates/proteins at 20 µg per lane.Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 53 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

MARCO, Polyclonal Antibody (Cat# AAA9206180)

Full Name
MARCO Antibody (N-term)
Gene Names
MARCO; SCARA2
Reactivity
Human
Applications
WB, EIA, FC/FACS, IHC-P
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Immunohistochemistry (IHC) (MBS9200563 staining CSF1R in human skin sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylatedgoat polyvalent antibody was used as the secondary antibody.)

CSF1R, Monoclonal Antibody (Cat# AAA9200563)

Full Name
CSF1R Antibody
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
WB, EIA, FC, IHC-P
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB) (Western blot analysis of lysates from H-4-II-E, mouse NIH/3T3 cell line (from left to right), using HSPA8 Antibody (C-term). MBS9208423 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

HSPA8, Polyclonal Antibody (Cat# AAA9208423)

Full Name
HSPA8 Antibody (C-term)
Gene Names
HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p
Reactivity
Human (Predicted Reactivity: Bovine, Hamster, Mouse, Rat)
Applications
IF, EIA, IHC, FC/FACS, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot analysis of lysate from SW480 cell line, using PCSK9 Antibody (N-term)(Cat.#MBS9213274). MBS9213274 was diluted at 1:2000. Agoat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

PCSK9, Polyclonal Antibody (Cat# AAA9213274)

Full Name
PCSK9 Antibody (N-term)
Gene Names
PCSK9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3
Reactivity
Human
Applications
WB, IHC-P, FC, EIA
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot analysis of lysate from NCCIT cell line, using SOX2 Antibody. AM2048a was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20?g.)

SOX2, Monoclonal Antibody (Cat# AAA9200597)

Full Name
SOX2 Antibody
Gene Names
SOX2; ANOP3; MCOPS3
Reactivity
Human
Applications
WB, IF, IHC-P, FC/FACS
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$435/0.4 mL | $190/0.08 mL | $1,705/5x0.4 mL
Western Blot (WB) (All lanes : Anti-Phospho-Rb-like-1(S975) Antibody (upper) at 1:250 dilution + Hela whole cell lysates, Beta-actin (lower)Lane 1: G0-phase?serum-deprived 24h?Lane 2: S-phase?hydroxycarbamide?5mM, 24hLane 3: G2/M-phase?etoposide?5mM, 24hLane 4: M-phase, nocodazole, 150ng/ml 20h Lysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 121 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Phospho-Rb-like-1 (S975), Polyclonal Antibody (Cat# AAA9214765)

Full Name
Phospho-Rb-like-1 (S975) Antibody
Gene Names
RBL1; PRB1; p107; CP107
Reactivity
Reactivity: Human
Predicted Reactivity: Mouse
Applications
WB, EIA, IHC-P
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (All lanes : Anti-DLK1 Antibody (C-term) at 1:1000 dilutionLane 1: human placenta lysatesLane 2: mouse skeletal muscle lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

DLK1, Polyclonal Antibody (Cat# AAA9210091)

Full Name
DLK1 Antibody (C-term)
Gene Names
DLK1; DLK; FA1; ZOG; pG2; DLK-1; PREF1; Delta1; Pref-1
Reactivity
Human, mouse
Applications
WB, EIA, IHC, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (Western blot analysis of lysate from SH-SY5Y cell line, using APG5L Antibody (D3). AP1812a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

ATG5, Polyclonal Antibody (Cat# AAA9206419)

Full Name
ATG5 Antibody (N-term)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human;
(Predicted Reactivity: Zebrafish, Bovine, Mouse, Pig, Rat, Rabbit )
Applications
WB, EIA, IHC-P, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (Anti-BHLH3 Antibody (N-term) at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

BHLH3, Polyclonal Antibody (Cat# AAA9208829)

Full Name
BHLH3 Antibody (N-term)
Gene Names
BHLHE41; DEC2; hDEC2; BHLHB3; SHARP1
Reactivity
Human, Mouse
Applications
FC, IF, WB, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H. kidney section using UCHL1. MBS9200054 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

UCHL1, Monoclonal Antibody (Cat# AAA9200054)

Full Name
UCHL1 Antibody
Gene Names
UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5
Reactivity
Human
Applications
EIA, IHC, IF, WB
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (All lanes : Anti-IL2RG Antibody (N-term) at 1:2000 dilutionLane 1: human spleen lysatesLane 2: Jurkat whole cell lysatesLane 3: Daudi whole cell lysatesLane 4: KG-1 whole cell lysatesLane 5: MOLT-4 whole cell lysatesLane 6: HL-60 whole cell lysates Lysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

IL2RG, Polyclonal Antibody (Cat# AAA9214003)

Full Name
IL2RG Antibody (N-term)
Gene Names
IL2RG; P64; CIDX; IMD4; CD132; SCIDX; IL-2RG; SCIDX1
Reactivity
Human
Applications
WB, EIA, FC/FACS, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Western blot analysis of lysate from 293T cell line, using CD38 Antibody (C-term). MBS9206190 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

CD38, Polyclonal Antibody (Cat# AAA9206190)

Full Name
CD38 Antibody (C-term)
Gene Names
CD38; T10; ADPRC 1
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using MBS9210328 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

FOLR1, Polyclonal Antibody (Cat# AAA9210328)

Full Name
FOLR1 Antibody (N-term)
Gene Names
FOLR1; FBP; FOLR
Reactivity
Human
Applications
FC/FACS, EIA, IHC, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilutionLane 1: mouse testis lysatesLane 2: mouse liver lysatesLane 3: NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 54 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

M Mlkl, Polyclonal Antibody (Cat# AAA9206965)

Full Name
M Mlkl Antibody (C-term)
Gene Names
Mlkl; 9130019I15Rik
Reactivity
Mouse
Applications
WB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of mouse liver, using HNF4A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

HNF4A, Polyclonal Antibody (Cat# AAA9134008)

Full Name
HNF4A Polyclonal Antibody
Gene Names
HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
Reactivity
Human, Mouse
Applications
WB, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Anti-Nestin Antibody (S1409)at 1:2000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 177 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Nestin, Polyclonal Antibody (Cat# AAA9203158)

Full Name
Nestin Antibody (S1409)
Gene Names
NES; Nbla00170
Reactivity
Human
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Peptide AffinityPurified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Testing Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)

V5-TAG, Monoclonal Antibody (Cat# AAA214958)

Full Name
MOUSE ANTI V5-TAG:HRP
Applications
EIA, WB
Pricing
$490/0.1 mg | $2,060/5x0.1 mg
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H. kidney section using HAS2 Antibody (Center). MBS9207356 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

HAS2, Polyclonal Antibody (Cat# AAA9207356)

Full Name
HAS2 Antibody (Center)
Reactivity
Human, mouse
Applications
WB, EIA, IHC-P, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H. cerebellum section using GLD2 Antibody (N-term). MBS9212203 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

GLD2, Polyclonal Antibody (Cat# AAA9212203)

Full Name
GLD2 Antibody (N-term)
Gene Names
PAPD4; GLD2; TUT2
Applications
EIA, IHC-P, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB) (Western blot analysis of lysate from Ramos cell line, using CD19Antibody (N-term). MBS9204578 was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

CD19, Polyclonal Antibody (Cat# AAA9204578)

Full Name
CD19 Antibody (N-term)
Gene Names
CD19; B4; CVID3
Reactivity
Human
Applications
WB, IF, FC, IHC-P, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Testing Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)

V5-TAG, Monoclonal Antibody (Cat# AAA210664)

Full Name
MOUSE ANTI V5-TAG:FITC
Applications
IF
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Western Blot ( All lanes : Anti-Prohibitin (PHB1) Antibody (N-term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole celllysate Lane 3: A431 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: Hela whole cell lysate Lane 6: Mouse kidney tissue lysate Lane 7: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Prohibitin (PHB1), Polyclonal Antibody (Cat# AAA9202298)

Full Name
Prohibitin (PHB1) Antibody (N-term)
Gene Names
PHB; PHB1; HEL-215; HEL-S-54e
Reactivity
Human, Mouse
Predicted Reactivity: Bovine, Chicken
Applications
FC/FACS, EIA, WB, IHC-P
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CD68 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)

CD68, Polyclonal Antibody (Cat# AAA9133332)

Full Name
CD68 Polyclonal Antibody
Gene Names
CD68; GP110; LAMP4; SCARD1
Reactivity
Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunohistochemistry (IHC) ( Immunohistochemical analysis of paraffin-embedded H. liver section using PAH Antibody (Center). MBS9200125 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

PAH, Monoclonal Antibody (Cat# AAA9200125)

Full Name
PAH Antibody (Center)
Gene Names
PAH; PH; PKU; PKU1
Reactivity
Human, mouse, rat (Predicted Reactivity: Bovine)
Applications
WB, EIA, IHC
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot analysis of extracts of mouse kidney, using MOCS2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

MOCS2, Antibody (Cat# AAA128659)

Full Name
MOCS2
Gene Names
MOCS2; MPTS; MCBPE; MOCO1
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis of lysate from HepG2 cell line, using PCSK9 Antibody (C-term). MBS9214204 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

PCSK9, Polyclonal Antibody (Cat# AAA9214204)

Full Name
PCSK9 Antibody (C-term)
Gene Names
PCSK9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3
Reactivity
Human
Applications
EIA, IHC-p, FC, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC) (Immunohistochemical analysis of MBS9203877 on paraffin-embedded human kidney tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.)

NEU2, Polyclonal Antibody (Cat# AAA9203877)

Full Name
NEU2 Antibody (N-term)
Gene Names
NEU2; SIAL2
Reactivity
Human
Applications
WB, EIA, IHC-P-Leica, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL

CCR7, Polyclonal Antibody (Cat# AAA9205978)

Full Name
CCR7 Antibody (N-term)
Gene Names
CCR7; BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
Reactivity
Human, mouse
Applications
WB, IHC-P, IF , FC, E
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using SLC7A2 antibody (MBS9134575) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

SLC7A2, Polyclonal Antibody (Cat# AAA9134575)

Full Name
SLC7A2 Polyclonal Antibody
Gene Names
SLC7A2; CAT2; ATRC2; HCAT2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (All lanes : Anti-XRN1 Antibody at 1:2000 dilutionLane 1: K562 whole cell lysateLane 2: MCF-7 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 194 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

XRN1, Monoclonal Antibody (Cat# AAA9216876)

Full Name
XRN1 Antibody
Gene Names
XRN1; SEP1
Reactivity
Human
Applications
WB
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Immunohistochemistry (IHC) (Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE))

FOLR1/Folate Receptor Alpha, Polyclonal Antibody (Cat# AAA2401574)

Full Name
Anti-FOLR1/Folate Receptor Alpha Antibody
Gene Names
FOLR1; FBP; FOLR
Reactivity
Human
Applications
FC/FACS, IHC, WB
Purity
Protein A purified
Pricing
$585/0.2 mL | $2,585/5x0.2 mL
Western Blot (Anti-ENPP2 Antibody (Center) at 1:2000 dilution + Human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 99 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

ENPP2, Polyclonal Antibody (Cat# AAA9202571)

Full Name
ENPP2 Antibody (Center)
Gene Names
ENPP2; ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA
Reactivity
Human
Applications
WB, EIA, FC/FACS
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using AVP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

AVP, Polyclonal Antibody (Cat# AAA9135577)

Full Name
AVP Polyclonal Antibody
Gene Names
AVP; VP; ADH; ARVP; AVRP; AVP-NPII
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Flow Cytometry (FC) (Flow cytometric analysis of U-87 MG cells using RAC1(green) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)

RAC1, Monoclonal Antibody (Cat# AAA9200589)

Full Name
RAC1 Antibody
Gene Names
RAC1; MIG5; Rac-1; TC-25; p21-Rac1
Reactivity
Human, rat (Predicted Reactivity: Bovine, Mouse)
Applications
WB, EIA, FC, IHC
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Test Data (All lanes: Anti-CD81 Antibody at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Ramos whole cell lysate Lane 3: U-87 MG whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

CD81, Monoclonal Antibody (Cat# AAA9217050)

Full Name
CD81 Antibody
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human
Applications
WB, EIA
Pricing
$190/0.05 mL | $435/0.2 mL | $1,705/5x0.2 mL
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H. astroglioma section using NCAM1 Antibody (C-term). MBS9207972 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

NCAM1, Polyclonal Antibody (Cat# AAA9207972)

Full Name
NCAM1 Antibody (C-term)
Gene Names
NCAM1; CD56; NCAM; MSK39
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat)
Applications
FC/FACS, IHC-P, WB, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (All lanes : Anti-OGN Antibody (N-term K75) at 1:500 dilutionLane 1: CCRF-CEM whole cell lysateLane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

OGN, Polyclonal Antibody (Cat# AAA9202306)

Full Name
OGN Antibody (C-term)
Gene Names
OGN; OG; OIF; SLRR3A
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
WB, EIA, IHC-P, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with EGF, 100ng/ml, using Phospho-SHP2-Y546 Antibody(EV)(upper) or Beta-actin (lower).)

SHP2, Polyclonal Antibody (Cat# AAA9231732)

Full Name
Phospho-SHP2 (Y546) Antibody
Gene Names
PTPN11; CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3
Reactivity
Human, Mouse
Predicted: Chicken, Rat
Applications
WB, DB, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL

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