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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for " phosphorylation" - showing 5950-6000
protein phosphatase 1, regulatory (inhibitor) subunit 15B, ELISA Kit (Cat# AAA9320678)
Full Name
Mouse Protein phosphatase 1 regulatory subunit 15B, PPP1R15B ELISA Kit
Gene Names
Ppp1r15b; CReP; AI606441; 1810033K10Rik; C530022L24Rik
Reactivity
Mouse
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using Myogenic Factor 5 (Phospho-Ser49) antibody.)
Myogenic Factor 5, Antibody (Cat# AAA859833)
Full Name
Myogenic Factor 5 (Phospho-Ser49) Antibody
Gene Names
MYF5; bHLHc2
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from rat liver cells, using Claudin 7 (Phospho-Tyr210) antibody.)
Claudin 7, Antibody (Cat# AAA853404)
Full Name
Claudin 7 (Phospho-Tyr210) Antibody
Gene Names
CLDN7; CLDN-7; CEPTRL2; CPETRL2; Hs.84359; claudin-1
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Flow Cytometry (FC/FACS)
(Staining of CD3- C57BL/6 splenocytes with CD49b antibody and 0.125 ug of Mouse IgG2a kappa Isotype Control (PE) (left) or 0.25 ug of Ly-49A antibody (PE) (right).)
Ly49A, Monoclonal Antibody (Cat# AAA832833)
Full Name
Ly49A antibody (PE)
Applications
FC/FACS
Pricing
$750/0.1 mg | $3,375/5x0.1 mg
Testing Data
(Peptide-+)
HtrA2, Antibody (Cat# AAA855785)
Full Name
HtrA2 (Ab-142) Antibody
Gene Names
HTRA2; OMI; MGCA8; PARK13; PRSS25
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-alpha (Ab-167) antibody.)
Estrogen Receptor-alpha, Antibody (Cat# AAA856720)
Full Name
Estrogen Receptor-alpha (Ab-167) Antibody
Gene Names
ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
Reactivity
Human
Applications
WB, IHC, IF
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using lkappaB-beta (Phospho-Ser23) antibody (left) or the same antibody preincubated with with blocking peptide (right))
IKB beta, Polyclonal Antibody (Cat# AAA835125)
Full Name
IKB beta antibody
Applications
IHC
Purity
IKB beta antibody was purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MBS627738.)
Estrogen Receptor, alpha, Polyclonal Antibody (Cat# AAA627738)
Full Name
Estrogen Receptor, alpha (ER)
Gene Names
ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
Reactivity
Human
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$480/0.1 mg | $2,150/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Interferon gamma ntibody at at 2 /mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 18.3 kDaObserved band size: 27 kDa Additional bands at: 70kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Interferon gamma, Polyclonal Antibody (Cat# AAA715642)
Full Name
Rabbit anti-human Interferon gamma polyclonal Antibody, HRP conjugated
Gene Names
IFNG; IFG; IFI
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(SIGLEC10 Antibody (C-term) (MBS9210889) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the SIGLEC10 antibody detected the SIGLEC10 protein (arrow).)
SIGLEC10, Polyclonal Antibody (Cat# AAA9210889)
Full Name
SIGLEC10 Antibody (C-term)
Gene Names
SIGLEC10; SLG2; PRO940; SIGLEC-10
Reactivity
Human
Applications
WB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Typical Testing Data/Standard Curve (for reference only)
Phosphorylation P38 Mitogen-activated Protein Kinase, ELISA Kit (Cat# AAA734513)
Full Name
Canine Phosphorylation P38 Mitogen-activated Protein Kinase ELISA Kit
Reactivity
Canine
Pricing
$475/48-Strip-Wells | $680/96-Strip-Wells | $3,010/5x96-Strip-Wells | $5,675/10x96-Strip-Wells
Western Blot (WB)
(Western blot analysis of extract from 3T3 cell using MBS622879, (lane 1 and 2) and Rabbit Anti-HDAC4/HDAC5/HDAC9 (Phospho-Ser246/259/220) Polyclonal Antibody)
HDAC4/5/9, Polyclonal Antibody (Cat# AAA622879)
Full Name
HDAC4/5/9
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$480/0.1 mg | $2,150/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of various cells with Phospho-Rad17 (Ser645) Polyclonal Antibody )
Rad17, Polyclonal Antibody (Cat# AAA2536089)
Full Name
Phospho-Rad17 (S645) Polyclonal Antibody
Gene Names
RAD17; CCYC; R24L; RAD24; HRAD17; RAD17SP
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
PYGB, Polyclonal Antibody (Cat# AAA536156)
Full Name
PYGB antibody
Gene Names
PYGB; GPBB
Reactivity
Human
Applications
IHC
Purity
PYGB antibody was purified by affinity chromatography.
Pricing
$885/0.05 mg | $3,965/5x0.05 mg
Western Blot (WB)
(Western Blot analysis of RAT-KIDNEY cells using GluR-1 Polyclonal Antibody)
GluR-1, Polyclonal Antibody (Cat# AAA9402882)
Full Name
GluR-1 Polyclonal Antibody
Gene Names
GRIA1; GLUH1; GLUR1; GLURA; GluA1; HBGR1
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, IF, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Testing Data
Catenin-beta, Antibody (Cat# AAA858694)
Full Name
Catenin-beta (Ab-654) Antibody
Gene Names
CTNNB1; EVR7; CTNNB; MRD19; armadillo
Reactivity
Human, Mouse, Rat
Applications
WB, IF, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western Blot analysis od extracts from 293 cells untreated or treated with 10% serum)
MEK1, Polyclonal Antibody (Cat# AAA626535)
Full Name
MEK1, phosphorylated (Thr291) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-Activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1)
Gene Names
MEK1; ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK
Reactivity
Human
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$550/0.1 mg | $2,455/5x0.1 mg
Western Blot (WB)
(WB: Lamdba phosphatase treated (Lane 1) and untreated (Lane 2) affinity purified bacterial expressed rat nNOS/NOS I that had been phosphorylated by purified AMPK was probed with MBS629394 (0.5ug/ml).)
NOS I, Polyclonal Antibody (Cat# AAA629394)
Full Name
NOS I, phosphorylated (Ser1417) (Neuronal Nitric Oxide Synthase, bNOS, nNOS, NOS-1)
Reactivity
Human, Rat
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$875/0.1 mg | $3,920/5x0.1 mg
Immunohistochemistry (IHC)
(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p56Dok-2 (phospho-Tyr299) antibody.)
p56Dok-2, Antibody (Cat# AAA856924)
Full Name
p56Dok-2 (Phospho-Tyr299) Antibody
Gene Names
DOK2; p56DOK; p56dok-2
Reactivity
Human
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
()
Nck2, Monoclonal Antibody (Cat# AAA834026)
Full Name
Nck2 antibody
Applications
WB, IP
Purity
Nck2 antibody was purified by ammonium sulfate precipitation
Western Blot
(Western Blot analysis using IKBKB antibodyWestern Blot showing IKBKB antibody used against truncated IKBKB recombinant protein (1) and K562 cell lysate (2).)
IKBKB, Monoclonal Antibody (Cat# AAA830041)
Full Name
IKBKB antibody
Gene Names
IKBKB; IKK2; IKKB; IMD15; NFKBIKB; IKK-beta
Applications
EIA, WB
Pricing
$645/0.1 mL | $2,885/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from COS cells treated with UV or MMS, using Phospho-p53 (Ser6) Antibody (upper) or p53 Antibody #P1001-27A (lower).)
p53, Polyclonal Antibody (Cat# AAA626232)
Full Name
p53, phosphorylated (Ser6) (Tumor Suppressor Protein, Oncogene Protein)
Gene Names
TP53; P53; LFS1; TRP53; FLJ92943
Reactivity
Hamster, Human, Monkey, Mouse
Applications
EL/EIA, WB, IP, ICC
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
PDHK1, Polyclonal Antibody (Cat# AAA9416261)
Full Name
PDHK1(Phospho-Thr338) Antibody
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
By immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(PER1 Antibody (C-term) (MBS9207968) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PER1 antibody detected the PER1 protein (arrow).)
PER1, Polyclonal Antibody (Cat# AAA9207968)
Full Name
PER1 Antibody (C-term)
Gene Names
PER1; PER; hPER; RIGUI
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
WB, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
G3BP-1, Polyclonal Antibody (Cat# AAA625640)
Full Name
G3BP-1 (Ras GTPase-Activating Protein-Binding Protein 1, GAP SH3 Domain-Binding Protein 1, ATP-dependent DNA helicase VIII, HDH-VIII, G3BP1, G3BP)
Reactivity
Human
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Testing Data
(Fig: Western blot analysis on mouse brain using anti-CD34 polyclonal antibody)
CD34, Polyclonal Antibody (Cat# AAA857333)
Full Name
CD34 Antibody
Reactivity
Mouse, Human, Rat
Applications
WB, ICC
Purity
Affinity purified
Pricing
$310/0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from NIH-3T3 cells treated with PMA using Phospho-Glycogen Synthase (Ser645) antibody.The lane on the right is treated with the antigen-specific peptide.)
Glycogen Synthase, Polyclonal Antibody (Cat# AAA9404770)
Full Name
Glycogen Synthase (Phospho-Ser645) Antibody
Gene Names
GYS1; GSY; GYS
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Hela (left), THP1 (middle) and 3T6 cell lysate (30 ug))
HDAC5, Polyclonal Antibody (Cat# AAA623861)
Full Name
HDAC5, phosphorylated (Ser498) (HD5, FLJ90614, KIAA0600, Antigen NY-CO-9, Histone Deacetylase 5)
Gene Names
HDAC5; HD5; NY-CO-9; FLJ90614
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using N2302-001T.)
NFkB, p65, Polyclonal Antibody (Cat# AAA630270)
Full Name
NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA)
Gene Names
NKIRAS2; KBRAS2; kappaB-Ras2
Reactivity
Human
Applications
EL/EIA, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$480/0.1 mg | $2,150/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts from A549 cells, using AKT1/3 (Ab-437/434) antibody.)
AKT1/3, Antibody (Cat# AAA854202)
Full Name
AKT1/3 (Ab-437/434) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells using BRCA2 (Phospho-Ser3291) Antibody.The lane on the right is treated with the antigen-specific peptide.)
BRCA2, Polyclonal Antibody (Cat# AAA9412619)
Full Name
BRCA2 (Phospho-Ser3291) Antibody
Gene Names
BRCA2; FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, treated with Ca+ (40nM, 30mins), using MYOD (Phospho-Ser200) antibody.)
MYOD, Antibody (Cat# AAA859407)
Full Name
MYOD (Phospho-Ser200) Antibody
Gene Names
MYOD1; PUM; MYF3; MYOD; bHLHc1
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from HuvEc cells, treated with Serum (20%, 30mins), using Histone H3 (Phospho-Ser28) antibody (#A0435).)
Histone H3, Antibody (Cat# AAA852974)
Full Name
Histone H3 (Phospho-Ser28) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Helicobacter pylori, Positive Control (Cat# AAA542852)
Full Name
Helicobacter pylori Positive Control
Gene Names
OPTN; NRP; FIP2; HIP7; HYPL; ALS12; GLC1E; TFIIIA-INTP
Applications
WB
Pricing
$335/5 Applications | $1,350/5x5 Applications
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, treated with UV (5mins), using JNK1/2/3 (Phospho-Thr183+Tyr185) antibody.)
JNK1/2/3, Antibody (Cat# AAA859477)
Full Name
JNK1/2/3 (Phospho-Thr183+Tyr185) Antibody
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data
(P-Peptide - +Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ASK1 (phospho- Ser966) antibody (E011179).)
ASK1, Antibody (Cat# AAA857224)
Full Name
ASK1 (Phospho-Ser966) Antibody
Gene Names
MAP3K5; ASK1; MEKK5; MAPKKK5
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot All lanes: Ubiquitin thioesterase OTUB1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 20 kDa Observed band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Ubiquitin thioesterase OTUB1, Polyclonal Antibody (Cat# AAA719480)
Full Name
Rabbit anti-human Ubiquitin thioesterase OTUB1 polyclonal Antibody, FITC
Gene Names
OTUB1; OTB1; OTU1; HSPC263
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Tax1-binding protein 3 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 13kDa Observed band size: 70 kDa Additional bands at: 36 kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Tax1-binding protein 3, Polyclonal Antibody (Cat# AAA715727)
Full Name
Rabbit anti-human Tax1-binding protein 3 polyclonal Antibody, HRP conjugated
Gene Names
TAX1BP3; TIP-1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
phosphorylated adaptor for RNA export, ELISA Kit (Cat# AAA9334796)
Full Name
Chicken Phosphorylated adapter RNA export protein, PHAX ELISA Kit
Gene Names
PHAX; RNUXA
Reactivity
Chicken
Protein Kinase C, theta, Polyclonal Antibody (Cat# AAA623870)
Full Name
Protein Kinase C, theta (Protein Kinase C theta Type, nPKC-theta, PRKCQ, PRKCT)
Gene Names
PRKCQ; PRKCT; MGC126514; MGC141919; nPKC-theta
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Western Blot (WB)
(Western blot analysis of extract from Hela cells using Rabbit Anti-Synaptotagmin I/II (Ab-202/199) Polyclonal Antibody (GenScript, A01170, lane 1 and 2)
Synaptotagmin I/II, Polyclonal Antibody (Cat# AAA620319)
Full Name
Synaptotagmin I/II (Syt)
Gene Names
SYT2; SytII; FLJ42519
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$480/0.1 mg | $2,150/5x0.1 mg
Cortactin, Polyclonal Antibody (Cat# AAA625834)
Full Name
Cortactin (CTTN)
Gene Names
CTTN; EMS1; FLJ34459
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
FKHR, Polyclonal Antibody (Cat# AAA627434)
Full Name
FKHR, phosphorylated (Ser256) (Forkhead Box Protein O1, Forkhead Box Protein O1A, Forkhead In Rhabdomyosarcoma, FOXO1A, FOXO1)
Gene Names
FOXO1; FKH1; FKHR; FOXO1A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Western Blot (WB)
(Western blot analysis of Akt1 phosphorylation expression in PDGF treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Akt1, Antibody (Cat# AAA8511442)
Full Name
Phospho-Akt1 (Thr450) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
CDC2, Polyclonal Antibody (Cat# AAA630007)
Full Name
CDC2, phosphorylated (Tyr15) (Cell Division Protein Kinase 1, Cell Division Control Protein 2 Homolog, Cyclin-dependent Kinase 1, CDK1, p34 Protein Kinase, CDK1)
Gene Names
cdc2; 5363; cdc; Cdc2; CDC2; cdc2Dm; CDCDm; cdk1; Cdk1; CDK1; CDK1/CDC2; CG5363; Dcdc2; Dm cdc2; Dmcdc2; DmCdc2; DmCdk1; DmelCG5363; group 4; l(2)31Eh
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IRS-1 (Phospho-Ser612) Antibody.)
IRS-1, Antibody (Cat# AAA858611)
Full Name
IRS-1 (Phospho-Ser612) Antibody
Gene Names
Irs1; G972R; IRS-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (Ab-243) antibody.)
c-Jun, Antibody (Cat# AAA857622)
Full Name
c-Jun (Ab-243) Antibody
Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot detection of MEK1/2 in A549, MCF7 and Hela cell lysates using MEK1/2 mouse mAb (dilution 1:500).Predicted band size:45kDa.Observed band size:45kDa.)
MEK1/2, Monoclonal Antibody (Cat# AAA475421)
Full Name
Anti-MEK1/2 Mouse mAb
Gene Names
MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
Western Blot (WB)
(Western blot Neutrophil gelatinase-associated lipocalin Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size:22 kDaObserved band size: 45 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
NGAL, Polyclonal Antibody (Cat# AAA715519)
Full Name
Rabbit anti-human NGAL polyclonal Antibody, HRP conjugated
Gene Names
LCN2; p25; 24p3; MSFI; NGAL
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Prefoldin subunit 5 antibody at at 2 /mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 17 kDaObserved band size: 70 kDa Additional bands at: 80kDa. We are unsure as to the identity of these extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Prefoldin subunit 5, Polyclonal Antibody (Cat# AAA715290)
Full Name
Rabbit anti-human Prefoldin subunit 5 polyclonal Antibody, Biotin conjugated
Gene Names
PFDN5; MM1; MM-1; PFD5
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
