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5611 results for "Protein A IgG Binding Buffer" - showing 5600-5611
Western Blot (WB)
(Western blot analysis of extracts from normal (control) and Calreticulineticulin knockout (KO) HeLa cells, using Calreticulineticulin antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Calreticulin, Polyclonal Antibody (Cat# AAA9143144)
Full Name
[KO Validated] Calreticulin Rabbit pAb
Gene Names
CALR; RO; CRT; SSA; cC1qR
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP
Purity
Affinity purification
Pricing
$210/0.02 mL | $265/0.05 mL | $380/0.1 mL | $540/0.2 mL | $2,285/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NLRP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
NLRP1, Polyclonal Antibody (Cat# AAA9142224)
Full Name
NLRP1 Rabbit pAb
Gene Names
NLRP1; NAC; CARD7; CIDED; NALP1; SLEV1; DEFCAP; PP1044; VAMAS1; CLR17.1; DEFCAP-L/S
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from normal (control) and HSP27/HSP27/HSPB1 knockout (KO) HeLa cells, using HSP27/HSP27/HSPB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)
HSP27/HSPB1, Polyclonal Antibody (Cat# AAA9142272)
Full Name
[KO Validated] HSP27/HSPB1 Rabbit pAb
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
$210/0.02 mL | $265/0.05 mL | $380/0.1 mL | $540/0.2 mL | $2,285/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PKM2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
PKM2, Polyclonal Antibody (Cat# AAA9142417)
Full Name
PKM2 Rabbit pAb
Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of mouse brain, using RTN4 antibody (MBS9144275) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 5min.)
RTN4, Polyclonal Antibody (Cat# AAA9144275)
Full Name
RTN4 Rabbit pAb
Gene Names
RTN4; ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Filamin A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Filamin A, Polyclonal Antibody (Cat# AAA9142294)
Full Name
Filamin A Rabbit pAb
Gene Names
FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western Blot using anti-CD155 antibody 3F1 (MBS488666). Mouse thymus lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 3F1 (MBS488666) at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
CD155, Monoclonal Antibody (Cat# AAA488667)
Full Name
Anti-CD155 [3F1]
Reactivity
Mouse
Applications
FC/FACS, EIA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-IL-8RA antibody SE2 (MBS488768) Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of SE2 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
IL-8RA, Monoclonal Antibody (Cat# AAA488768)
Full Name
Anti-IL-8RA [SE2]
Gene Names
CXCR1; C-C; CD128; CD181; CKR-1; IL8R1; IL8RA; CMKAR1; IL8RBA; CDw128a; C-C-CKR-1
Reactivity
Human
Applications
WB, FC/FACS, IHC
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western Blot using anti-HVEM antibody HMHV-1B18 (MBS488601) Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 () at 0.01ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
HVEM, Monoclonal Antibody (Cat# AAA488601)
Full Name
Anti-HVEM [HMHV-1B18]
Reactivity
Mouse
Applications
FC/FACS, WB, IP, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-CD25 antibody 2E4 (MBS488869). Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of 2E4 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
CD25, Monoclonal Antibody (Cat# AAA488869)
Full Name
Anti-CD25 [2E4]
Gene Names
Il2ra; CD25; Il2r; Ly-43
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-CD25 antibody 2E4 (MBS488869). Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of 2E4 (MBS488869, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
CD25, Monoclonal Antibody (Cat# AAA488868)
Full Name
Anti-CD25 [2E4]
Gene Names
Il2ra; CD25; Il2r; Ly-43
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
