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5611 results for "Protein A IgG Binding Buffer" - showing 5300-5350

Immunohistochemistry (IHC) (IHC image diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)

SYNCRIP, Monoclonal Recombinant Antibody (Cat# AAA7136847)

Full Name
SYNCRIP Antibody
Gene Names
SYNCRIP; PP68; NSAP1; GRYRBP; HNRNPQ; HNRPQ1; GRY-RBP; hnRNP-Q
Reactivity
Human
Applications
EIA, IHC, IF
Purity
Affinity-chromatography
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL
Western Blot (WB) (Western blot analysis of HMG17 (Phospho-Ser29) using EGF treated NIH-3T3 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

HMG17, Polyclonal Antibody (Cat# AAA9614066)

Full Name
Phospho-HMG17 (Ser29) Antibody
Gene Names
HMGN2; HMG17
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from HeLa  , using Phospho-RapGEF1 (Thr1071) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

RapGEF1, Polyclonal Antibody (Cat# AAA9613269)

Full Name
Phospho-RapGEF1 (Thr1071) Antibody
Gene Names
RAPGEF1; C3G; GRF2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (91%), Rabbit (91%), Dog (100%), Chicken (100%), Xenopus (82%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from H2O2 treated C6 cells, using Phospho-TAB1 (Ser438) Antibody. The lane on the left was treated with blocking peptide.)

TAB1, Polyclonal Antibody (Cat# AAA9614325)

Full Name
Phospho-TAB1 (Ser438) Antibody
Gene Names
TAB1; 3'-Tab1; MAP3K7IP1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of MAP4 (Phospho-Ser696) using LOVO whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

MAP4, Polyclonal Antibody (Cat# AAA9614076)

Full Name
Phospho-MAP4 (Ser696) Antibody
Gene Names
MAP4; MGC8617; DKFZp779A1753
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western Blot using anti-CD74 antibody LL1 (EPB1) (MBS488790). Human spleen(A) (0.01ug/ml) and human tonsil(B) (0.05ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of LL1 (EPB1) () before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)

CD74, Monoclonal Antibody (Cat# AAA488790)

Full Name
Anti-CD74 [LL1 (EPB1)]
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Applications
RIA, FC/FACS, WB, IP
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB) (Western Blot Positive WB detected in: K562 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate All lanes: XRCC5 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 83 kDa Observed band size: 83 kDa)

XRCC5, Monoclonal Recombinant Antibody (Cat# AAA7136619)

Full Name
XRCC5 Antibody
Gene Names
XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
Reactivity
Human
Applications
EIA, WB, IHC, IF
Purity
Affinity-chromatography
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts from Mouse lung, using Phospho-A-RAF (Ser299) Antibody. The lane on the left was treated with blocking peptide.)

A-RAF, Polyclonal Antibody (Cat# AAA9614133)

Full Name
Phospho-A-RAF (Ser299) Antibody
Gene Names
ARAF; PKS2; A-RAF; ARAF1; RAFA1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (88%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (82%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, U-87 whole cell lysate, PC-3 whole cell lysate, L02 whole cell lysate, Rat Heart whole cell lysate All lanes: p38 MAPK antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 42, 35, 36, 30 kDa Observed band size: 42 kDa)

MAPK14, Monoclonal Recombinant Antibody (Cat# AAA7136730)

Full Name
MAPK14 Antibody
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Rat
Applications
EIA, WB, IHC, IF
Purity
Affinity-chromatography
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL
Western Blot (WB) (Western blot analysis of AMPKB1 (Phospho-Ser108) using UV treated Jurkat whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

AMPK beta 1, Polyclonal Antibody (Cat# AAA9614239)

Full Name
Phospho-AMPK beta 1 (Ser108) Antibody
Gene Names
PRKAB1; AMPK; HAMPKb
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (90%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of EIF6 (Phospho-Ser235) using HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

EIF6, Polyclonal Antibody (Cat# AAA9614467)

Full Name
Phospho-EIF6 (Ser235) Antibody
Gene Names
EIF6; CAB; EIF3A; eIF-6; p27BBP; ITGB4BP; b(2)gcn; p27(BBP)
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (89%), Dog (100%), Xenopus (89%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of ETS1 (Phospho-Ser282) using A549 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

ETS1, Polyclonal Antibody (Cat# AAA9614372)

Full Name
Phospho-ETS1 (Ser282) Antibody
Gene Names
ETS1; ETS-1; EWSR2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Figure 1. Western blot analysis of GNG4 using anti-GNG4 antibody (MBS1753835).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human SH-SY5Y whole cell lysatesLane 2: rat brain tissue lysatesLane 3: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-GNG4 antigen affinity purified polyclonal antibody (Catalog # MBS1753835) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for GNG4 at approximately 12KD. The expected band size for GNG4 is at 12KD. )

GNG4, Polyclonal Antibody (Cat# AAA1753835)

Full Name
Anti-GNG4 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts from A-431, using Phospho-Desmoplakin (Ser165/166) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Desmoplakin, Polyclonal Antibody (Cat# AAA9613210)

Full Name
Phospho-Desmoplakin (Ser165/Ser166) Antibody
Gene Names
DSP; DP; DPI; DPII
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (91%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from K562 cells, using Phospho-CD22 (Tyr842) Antibody. The lane on the left was treated with blocking peptide.Observed bands: 140kDa)

CD22, Polyclonal Antibody (Cat# AAA9614218)

Full Name
Phospho-CD22 (Tyr842) Antibody
Gene Names
CD22; SIGLEC2; SIGLEC-2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse (83%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of PRKCB1 (Phospho-Thr500) using Na3VO4 treated HepG2 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

PKC beta 1, Polyclonal Antibody (Cat# AAA9614397)

Full Name
Phospho-PKC beta 1 (Thr500) Antibody
Gene Names
PRKCB; PKCB; PRKCB1; PRKCB2; PKC-beta
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (88%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of MYPT1/2 (Phospho-Ser668/Ser618) using K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

MYPT1/2, Polyclonal Antibody (Cat# AAA9614443)

Full Name
Phospho-MYPT1/2 (Ser668/Ser618) Antibody
Gene Names
PPP1R12A; MBS; M130; MYPT1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Figure 1. Western blot analysis of P Glycoprotein/ABCB1 using anti-P Glycoprotein/ABCB1 antibody (MBS1753268).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat PC-12 whole cell lysatesLane 2: mouse liver tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-P Glycoprotein/ABCB1 antigen affinity purified polyclonal antibody (Catalog # MBS1753268) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for P Glycoprotein/ABCB1 at approximately 140KD. The expected band size for P Glycoprotein/ABCB1 is at 140KD. )

P Glycoprotein/ABCB1, Polyclonal Antibody (Cat# AAA1753268)

Full Name
Anti-P Glycoprotein/ABCB1 Antibody
Gene Names
ABCB1; CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of Galectin 2/LGALS2 using anti-Galectin 2/LGALS2 antibody (MBS1753642).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human U-87MG whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Galectin 2/LGALS2 antigen affinity purified polyclonal antibody (Catalog # MBS1753642) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Galectin 2/LGALS2 at approximately 15KD. The expected band size for Galectin 2/LGALS2 is at 15KD. )

Galectin 2/LGALS2, Polyclonal Antibody (Cat# AAA1753642)

Full Name
Anti-Galectin 2/LGALS2 Antibody
Gene Names
LGALS2; HL14
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts from Mouse lung, using Phospho-Caveolin 2 (Ser36) Antibody. The lane on the left was treated with blocking peptide.)

Caveolin 2, Polyclonal Antibody (Cat# AAA9614106)

Full Name
Phospho-Caveolin 2 (Ser36) Antibody
Gene Names
CAV2; CAV
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Figure 1. Western blot analysis of JAB1 using anti-JAB1 antibody (MBS1754000).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Raji whole cell lysatesLane 2: human K562 whole cell lysatesLane 3: human PC-3 whole cell lysatesLane 4: human Hela whole cell lysatesLane 5: rat brain tissue lysatesLane 6: rat RH35 whole cell lysatesLane 7: mouse brain tissue lysatesLane 8: mouse SP2/0 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- JAB1 antigen affinity purified monoclonal antibody (Catalog # MBS1754000) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for JAB1 at approximately 37KD. The expected band size for JAB1 is at 37KD. )

JAB1, Monoclonal Antibody (Cat# AAA1754000)

Full Name
Anti-JAB1 Antibody (monoclonal, 4G9)
Gene Names
COPS5; CSN5; JAB1; SGN5; MOV-34
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of hnRNP D/AUF1/HNRNPD using anti-hnRNP D/AUF1/HNRNPD antibody (MBS1754047).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human K562 whole cell lysatesLane 3: human A431 whole cell lysatesLane 4: human HEPG2 whole cell lysatesLane 5: human placenta tissue lysatesLane 6: rat brain tissue lysatesLane 7: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-hnRNP D/AUF1/HNRNPD antigen affinity purified monoclonal antibody (Catalog # MBS1754047) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for hnRNP D/AUF1/HNRNPD at approximately 43-45KD. The expected band size for hnRNP D/AUF1/HNRNPD is at 38KD. )

hnRNP D/AUF1/HNRNPD, Monoclonal Antibody (Cat# AAA1754047)

Full Name
Anti-hnRNP D/AUF1/HNRNPD Antibody (monoclonal, 4F3)
Gene Names
HNRNPD; P37; AUF1; AUF1A; HNRPD; hnRNPD0
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC) (Figure 2. IHC analysis of METTL3 using anti-METTL3 antibody (MBS1753460).METTL3 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL3 Antibody (MBS1753460) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )

METTL3, Polyclonal Antibody (Cat# AAA1753460)

Full Name
Anti-METTL3 Antibody
Gene Names
METTL3; M6A; IME4; Spo8; MT-A70
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of RAB1B using anti-RAB1B antibody (MBS1753666).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human HEK293 whole cell lysatesLane 3: human SKOV3 whole cell lysatesLane 4: human MCF-7 whole cell lysatesLane 5: human A375 whole cell lysatesLane 6: human U87 whole cell lysatesLane 7: human A549 whole cell lysaetsLane 8: rat kidney tissue lysatesLane 9: rat brain tissue lysatesLane 10: rat heart tissue lysatesLane 11: rat NRK whole cell lysatesLane 12: mouse brain tissue lysatesLane 13: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-RAB1B antigen affinity purified polyclonal antibody (Catalog # MBS1753666) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for RAB1B at approximately 24KD. The expected band size for RAB1B is at 24KD. )

RAB1B, Polyclonal Antibody (Cat# AAA1753666)

Full Name
Anti-RAB1B Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC) (At 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

UHRF1, Polyclonal Antibody (Cat# AAA9612621)

Full Name
Phospho-UHRF1 (Ser311) Antibody
Gene Names
UHRF1; Np95; hNP95; ICBP90; RNF106; hUHRF1
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of Phospho-PTEN (Ser380/Thr382/383) in lysates of NIH-3T3, using Phospho-PTEN (Ser380/Thr382/383) Antibody(AF4450).)

PTEN, Polyclonal Antibody (Cat# AAA9613251)

Full Name
Phospho-PTEN (Ser380+Thr382+Thr383) Antibody
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Figure 1. Western blot analysis of P4HB using anti-P4HB antibody (MBS1753516).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human PANC-1 whole cell lysatesLane 3: human MCF-7 whole cell lysatesLane 4: human COLO-320 whole cell lysatesLane 5: human HEK293 whole cell lysatesLane 6: human HepG2 whole cell lysatesLane 7: monkey lung tissue lysatesLane 8: monkey liver tissue lysatesLane 9: rat liver tissue lysatesLane 10: mouse liver tissue lysatesLane 11: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-P4HB antigen affinity purified polyclonal antibody (Catalog # MBS1753516) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for P4HB at approximately 57KD. The expected band size for P4HB is at 57KD. )

P4HB, Polyclonal Antibody (Cat# AAA1753516)

Full Name
Anti-P4HB Antibody
Gene Names
P4HB; DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; ERBA2L; P4Hbeta
Reactivity
Human, Monkey, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Western blot analysis FGFR1/2 (Phospho-Tyr730/733) using NIH-3T3 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

FGFR1/2, Polyclonal Antibody (Cat# AAA9614227)

Full Name
Phospho-FGFR1/2 (Tyr730/Tyr733) Antibody
Gene Names
FGFR1; CEK; FLG; HH2; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; bFGF-R-1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (88%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of PSEN1 (Phospho-Ser353) using TNF-α treated HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Presenilin 1, Polyclonal Antibody (Cat# AAA9614305)

Full Name
Phospho-Presenilin 1 (Ser353) Antibody
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse (89%), Rabbit (90%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL

Vedolizumab, Inhibitor (Cat# AAA5759559)

Full Name
Vedolizumab
Purity
99.6%
Pricing
$670/5 mg | $2,715/5x5 mg
Testing Data (All lanes : Anti-RAB14 Antibody at 1:2000 dilutionnLane 1: Hela whole cell lysatenLane 2: Raji whole cell lysatenLane 3: human brain lysatennLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. nnPredicted band size : 24 kDannBlocking/Dilution buffer: 5% NFDM/TBST.)

Rab14, Monoclonal Antibody (Cat# AAA4754086)

Full Name
Rab14 (7A6) Mouse mAb
Gene Names
RAB14; FBP; RAB-14
Reactivity
Human
Applications
WB
Purity
Affinity purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using RPL35 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)

RPL35, Polyclonal Antibody (Cat# AAA9142893)

Full Name
RPL35 Rabbit pAb
Gene Names
RPL35; L35
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Testing Data (Fig.1. Protein was extracted from the non-denaturing Lysis Buffer, then it was verified by Co-IP. While the whole cell lysates (Input) and Co-IP samples were validated with Stat1 monoclonal antibody, Stat2 polyclonal antibody, and GAPDH monoclonal antibody respectively by WB.)

Universal IP/Co-IP Tool (Agarose), Purification Kit (Cat# AAA9719230)

Full Name
Universal IP/Co-IP Tool kit (Agarose)
Applications
IP
Pricing
$230/20 Tests | $1,040/5x20 Tests
Immunohistochemistry (IHC) (IHC image diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)

S100A9, Monoclonal Recombinant Antibody (Cat# AAA7136822)

Full Name
S100A9 Antibody
Reactivity
Human
Applications
EIA, IHC
Purity
Affinity-chromatography
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL
Western Blot (WB) (Western Blot Positive WB detected in: 293 whole cell lysate All lanes: JUN antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 43 kDa)

JUN, Monoclonal Recombinant Antibody (Cat# AAA7136779)

Full Name
JUN Antibody
Gene Names
JUN; AP1; AP-1; c-Jun
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Affinity-chromatography
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL
Chromatin Immunoprecipitation (ChIP) (RNA pol II antibody (mAb) (Clone 1F4B6) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from HL-60 cells and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 14 million sequence tags were mapped to identify RNA pol II binding sites. The image shows binding across a region of chromosome 15. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)

RNA pol II, Monoclonal Antibody (Cat# AAA389003)

Full Name
RNA pol II antibody (mAb)(Clone 1F4B6)
Gene Names
POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
Reactivity
Human
Applications
ChIP, ChIP-Seq, ICC, IF, WB
Purity
Protein G Chromatography
Pricing
$200/0.01 mg | $600/0.1 mg | $2,645/5x0.1 mg
Western Blot (WB) (Western blot analysis of CUTL1 (Phospho-Ser1237) using EGF treated A431 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

CUTL1, Polyclonal Antibody (Cat# AAA9614264)

Full Name
Phospho-CUTL1 (Ser1237) Antibody
Gene Names
CUX1; CDP; CUX; p75; CASP; CDP1; COY1; Clox; p100; p110; p200; CUTL1; GOLIM6; CDP/Cut; Cux/CDP; Nbla10317
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Immunohistochemistry (IHC) (IHC image diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)

ILF3, Monoclonal Recombinant Antibody (Cat# AAA7136635)

Full Name
ILF3 Antibody
Gene Names
ILF3; CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90
Reactivity
Human
Applications
EIA, IHC, IF
Purity
Affinity-chromatography
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts from mouse lung, using GAIP (Phospho-Ser151) Antibody.)

RGS19, Polyclonal Antibody (Cat# AAA9614275)

Full Name
Phospho-RGS19 (Ser151) Antibody
Gene Names
RGS19; GAIP; RGSGAIP
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (89%), Xenopus (89%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Immunohistochemistry (IHC) (Figure 1. IHC analysis of MRP5/ABCC5 using anti-MRP5/ABCC5 antibody (MBS1753570).MRP5/ABCC5 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MRP5/ABCC5 Antibody (MBS1753570) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )

MRP5/ABCC5, Polyclonal Antibody (Cat# AAA1753570)

Full Name
Anti-MRP5/ABCC5 Antibody
Gene Names
ABCC5; MRP5; SMRP; ABC33; MOATC; MOAT-C; pABC11; EST277145
Reactivity
Human
Applications
IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Flow Cytometry (FC/FACS) (Flow-cytometry using the anti-IgM M15/8 (MBS488541) Daudi cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M15/8 (, blue line) at a concentration of 10ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2ug/ml and cells analyzed on a FACSCanto flow-cytometer.)

IgM, Monoclonal Antibody (Cat# AAA488541)

Full Name
Anti-IgM [M15/8]
Gene Names
IGHM; MU; VH; AGM1
Reactivity
Human
Applications
EIA, FC/FACS, IHC-F, IP, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB) (Western Blot Positive WB detected in: 293 whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate All lanes: PABPN1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 33, 32, 38 kDa Observed band size: 50 kDa)

PABPN1, Monoclonal Recombinant Antibody (Cat# AAA7136687)

Full Name
PABPN1 Antibody
Gene Names
PABPN1; OPMD; PAB2; PABII; PABP2; PABP-2
Reactivity
Human
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
Affinity-chromatography
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL
Immunohistochemistry (IHC) (At 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

hFANCI, Polyclonal Antibody (Cat# AAA9612594)

Full Name
Phospho-hFANCI (Ser565) Antibody
Gene Names
FANCI; KIAA1794
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, using Twist2 Antibody. The lane on the left was treated with blocking peptide.)

Twist2, Polyclonal Antibody (Cat# AAA9613094)

Full Name
Twist2 Antibody
Gene Names
TWIST2; AMS; FFDD3; BBRSAY; DERMO1; SETLSS; bHLHa39
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (92%), Dog (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
$245/0.05 mL | $305/0.1 mL | $365/0.2 mL | $1,495/5x0.2 mL
Western Blot (WB) (Western blot analysis of AXL (Phospho-Tyr702) using 293 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

AXL, Polyclonal Antibody (Cat# AAA9614516)

Full Name
Phospho-AXL (Tyr702) Antibody
Gene Names
AXL; UFO; JTK11
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Immunofluorescence (IF) (Immunofluorescence staining of fixed Daudi cells with anti-CD74 antibody LN-2 (MBS488743) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of LN-2 (MBS488743) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488743, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)

CD74, Monoclonal Antibody (Cat# AAA488742)

Full Name
Anti-CD74 [LN-2]
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, FC/FACS, EIA, IP
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB) (Figure 1. Western blot analysis of GAP43 using anti-GAP43 antibody (MBS1753471).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysatesLane 3: mouse C6 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-GAP43 antigen affinity purified polyclonal antibody (Catalog # MBS1753471) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for GAP43 at approximately 45KD. The expected band size for GAP43 is at 24KD. )

GAP43, Polyclonal Antibody (Cat# AAA1753471)

Full Name
Anti-GAP43 Antibody
Gene Names
Gap43; B-50; Basp2; GAP-43
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Flow Cytometry (FC/FACS) (Figure 2. Flow Cytometry analysis of CACO-2 cells using anti-CARD4/NOD1 antibody (MBS1753333).Overlay histogram showing CACO-2 cells stained with MBS1753333 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CARD4/NOD1 Antibody (MBS1753333, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )

CARD4/NOD1, Polyclonal Antibody (Cat# AAA1753333)

Full Name
Anti-CARD4/NOD1 Antibody
Gene Names
NOD1; CARD4; NLRC1; CLR7.1
Reactivity
Human
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of GATA3 using anti-GATA3 antibody (MBS1753350).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-GATA3 antigen affinity purified polyclonal antibody (Catalog # MBS1753350) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for GATA3 at approximately 50KD. The expected band size for GATA3 is at 50KD. )

GATA3, Polyclonal Antibody (Cat# AAA1753350)

Full Name
Anti-GATA3 Antibody
Gene Names
GATA3; HDR; HDRS
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of TAB2 using anti-TAB2 antibody (MBS1753529).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human A549 whole cell lysatesLane 3: human HepG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-TAB2 antigen affinity purified polyclonal antibody (Catalog # MBS1753529) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for TAB2 at approximately 76KD. The expected band size for TAB2 is at 76KD. )

TAB2, Polyclonal Antibody (Cat# AAA1753529)

Full Name
Anti-TAB2 Antibody
Gene Names
TAB2; CHTD2; TAB-2; MAP3K7IP2
Reactivity
Human
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg

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