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10000 results for " phosphorylation" - showing 5250-5300


TIM3, Monoclonal Antibody (Cat# AAA833173)

Full Name
TIM3 antibody (PE)
Gene Names
HAVCR2; TIM3; CD366; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2
Applications
FC/FACS
Pricing
$420/0.05 mg | $1,870/5x0.05 mg
Immunohistochemistry (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT6 (phospho- Tyr641) antibody.)

STAT6, Antibody (Cat# AAA856939)

Full Name
STAT6 (Phospho-Tyr641) Antibody
Gene Names
STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
Reactivity
Human
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC) (P-peptide-+ Immunohistochemical analysis of paraffin-embedded human brain tissue using Trk A (phospho-Tyr791) antibody.)

Trk A, Antibody (Cat# AAA852157)

Full Name
Trk A (Phospho-Tyr791) Antibody
Gene Names
NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
Reactivity
Human
Applications
IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data

Keratin 18, Antibody (Cat# AAA859770)

Full Name
Keratin 18 (Phospho-Ser52) Antibody
Gene Names
KRT18; K18; CK-18; CYK18
Reactivity
Human
Applications
WB, IF, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB) (Western blot analysis of extracts from 3T3 cells untreated or treated with serum using PLC-g1(phospho-Tyr771) Antibody.)

PLC-gamma1, Polyclonal Antibody (Cat# AAA9409440)

Full Name
PLC-gamma1 (phospho-Tyr771) Antibody
Gene Names
PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Immunohistochemistry (IHC) (Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb (Ab-780) antibody.)

Rb, Antibody (Cat# AAA857233)

Full Name
Rb (Ab-780) Antibody
Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB) (Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using TR-beta1 (Phospho-Ser142) antibody.)

Tuberin, Antibody (Cat# AAA858743)

Full Name
Tuberin (Phospho-Ser981) Antibody
Gene Names
THRB; GRTH; PRTH; THR1; ERBA2; NR1A2; THRB1; THRB2; C-ERBA-2; C-ERBA-BETA
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Gene Sequencing (extract)

Caspase 4 (CASP4), Recombinant Protein (Cat# AAA2010519)

Full Name
Recombinant Caspase 4 (CASP4)
Gene Names
Casp4; Caspl; ich-3; Casp11
Reactivity
Mus musculus (Mouse)
Applications
WB
Purity
> 90%
Pricing
$145/0.01 mg | $250/0.05 mg | $365/0.1 mg | $450/0.2 mg | $850/0.5 mg | $1,250/1 mg | $3,055/5 mg | $13,745/5x5 mg
Testing Data (P-peptide-+)

MAP2K7, Antibody (Cat# AAA856388)

Full Name
MAP2K7 (Phospho-Ser271) Antibody
Gene Names
MAP2K7; MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)

Phosphorylation P38 Mitogen-activated Protein Kinase, ELISA Kit (Cat# AAA741650)

Full Name
Porcine Phosphorylation P38 Mitogen-activated Protein Kinase ELISA Kit
Reactivity
Porcine
Pricing
$475/48-Strip-Wells | $680/96-Strip-Wells | $3,010/5x96-Strip-Wells | $5,675/10x96-Strip-Wells
Western Blot (WB) (Western blot analysis of extract from mouse brain tissue, using APP (Ab-668) antibody and APP (Phospho-Thr668) antibody.)

APP, Antibody (Cat# AAA856952)

Full Name
APP (Ab-668) Antibody
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB) (Western blot analysis of extracts from 20% serum treated mouse NIH/3T3 cells on FKHR phosphorylation, using Phospho-FKHR (Ser256) Antibody (upper) or FKHR Antibody #9462 (lower).)

FKHR, Polyclonal Antibody (Cat# AAA625216)

Full Name
FKHR, phosphorylated (Ser256) (Forkhead Family Transcription Factor)
Gene Names
FOXO1; FKH1; FKHR; FOXO1A
Reactivity
Human, Mouse, Rat
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Western Blot (WB) (Western blot 60S ribosomal protein L36a-like Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 12 kDaObserved band size:20kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)

60S ribosomal protein L36a, Polyclonal Antibody (Cat# AAA715524)

Full Name
Rabbit anti-human 60S ribosomal protein L36a-like polyclonal Antibody, HRP conjugated
Gene Names
RPL36AL; RPL36A
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB) (Western blot All lanes: Phosphatidylserine synthase 1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 30 kDa Observed band size: 27kDa Additional bands at: 50 kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)

Cytochrome b-c1 complex subunit Rieske, Polyclonal Antibody (Cat# AAA715945)

Full Name
Rabbit anti-human Cytochrome b-c1 complex subunit Rieske, mitochondrial polyclonal Antibody, HRP conjugated
Gene Names
UQCRFS1; RIP1; RIS1; RISP; UQCR5
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB) (WB: Src Immunoprecipitated with anti-Src (05-184) was either treated (lane 1) or untreated (lanes 2 and 3) with the phosphotyrosine phosphatase YOP (14-229), then probed with anti-phospho-Src (lanes 1 & 2), or anti-Src (05-184) (lane 3).)

Src, Monoclonal Antibody (Cat# AAA632251)

Full Name
Src, phosphorylated (pp60Src, pp60c-Src)
Gene Names
Src; AW259666; pp60c-src
Reactivity
Human, Mouse, Rat
Applications
WB, IP
Purity
Purified by thilliophillic adsorption and size exclusion chromatography
Pricing
$900/0.1 mg | $4,045/5x0.1 mg

CDK6, Polyclonal Antibody (Cat# AAA628420)

Full Name
CDK6, phosphorylated (Tyr221) (Cell Division Protein Kinase 6, Serine/Threonine-protein Kinase PLSTIRE, Crk2, MGC59692)
Gene Names
CDK6; PLSTIRE; MGC59692
Reactivity
Human, Mouse, Rat
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Western Blot (WB) (Western blot analysis of extracts from HUVEC cells, MCF-7 cells and 293 cells, using Fyn (Ab-530) antibody.)

Fyn, Antibody (Cat# AAA854105)

Full Name
Fyn (Ab-530) Antibody
Gene Names
FYN; SLK; SYN; p59-FYN
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC) (Peptide-+ Immunohistochemical analysis of human paraffin-embedded human breast carcinoma tissue using ATF4 (Ab-245) antibody.)

ATF4, Antibody (Cat# AAA857186)

Full Name
ATF4 (Ab-245) Antibody
Gene Names
ATF4; CREB2; TXREB; CREB-2; TAXREB67
Reactivity
Human
Applications
IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data

DYR1A, Antibody (Cat# AAA856363)

Full Name
DYR1A Antibody
Gene Names
DYRK1A; MNB; DYRK; HP86; MNBH; MRD7; DYRK1
Reactivity
Human, Mouse, Rat
Applications
WB, IF, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)

Phosphorylation P38 Mitogen-Activated Protein Kinase, ELISA Kit (Cat# AAA25490)

Full Name
Human Phosphorylation P38 Mitogen-Activated Protein Kinase ELISA Kit
Reactivity
Human
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Testing Data (Peptide-+)

RIPK2, Antibody (Cat# AAA855982)

Full Name
RIPK2 (Ab-176) Antibody
Gene Names
RIPK2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)

phosphorylated adaptor for RNA export, ELISA Kit (Cat# AAA9338164)

Full Name
Bovine Phosphorylated adapter RNA export protein, PHAX ELISA Kit
Gene Names
PHAX; RNUXA
Reactivity
Bovine
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.)

Acetylated Lysine, Monoclonal Antibody (Cat# AAA803834)

Full Name
Acetylated Lysine Antibody: ATTO 594
Reactivity
Bovine, Avian
Applications
WB, EIA, IHC, IF
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.)

Acetylated Lysine, Monoclonal Antibody (Cat# AAA800676)

Full Name
Acetylated Lysine Antibody: ATTO 390
Reactivity
Bovine, Avian
Applications
WB, EIA, IHC, IF
Pricing
$480/0.1 mg | $1,935/5x0.1 mg

MARCKS, Polyclonal Antibody (Cat# AAA630298)

Full Name
MARCKS, phosphorylated (Ser162) (Myristolated Alanine Rich C Kinase Substrate, Myristoylated Alanine Rich Protein Kinase C Substrate, MACS, MARCS, 80K-L Protein, 81kD Protein Light Chain, MGC52672, Phosphomyristin, PKCSL, PRKCSL, Protein Kinase C Substrat
Gene Names
MARCKS; MACS; 80K-L; PKCSL; PRKCSL; FLJ14368; FLJ90045
Reactivity
Human, Mouse, Rat
Applications
IF
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Western Blot (WB) (Western blot All lanes: 40S ribosomal protein S9 antibody at at 2 /mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 21kDa Observed band size: 26kDa Additional bands at: 28kDa. We are unsure as to the identity of these extra bands. 40S ribosomal protein S9 contains a number of potential phosphorylation and acetylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.)

40S ribosomal protein S9, Polyclonal Antibody (Cat# AAA715798)

Full Name
Rabbit anti-human 40S ribosomal protein S9 polyclonal Antibody, HRP conjugated
Gene Names
RPS9; S9
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts from HuvEc cells (Lane 2), COS7 cells (Lane 3) and JK cells (Lane 4), using Androgen Receptor (Phospho-Tyr363) Antibody. The lane on the left is treated with antigen-specific peptide.)

Androgen Receptor, Polyclonal Antibody (Cat# AAA9404959)

Full Name
Androgen Receptor (Phospho-Tyr363) Antibody
Gene Names
AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
Reactivity
Human, Mouse
Applications
WB
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL

Histone H3.1, Polyclonal Antibody (Cat# AAA627646)

Full Name
Histone H3.1, phosphorylated (Thr3) (H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ
Gene Names
HIST1H3C; H3.1; H3/c; H3FC
Reactivity
Human, Mouse, Rat
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Testing Data (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad3 (Phospho-Ser425) Antibody (E011325).)

Smad3, Antibody (Cat# AAA857591)

Full Name
Smad3 (Phospho-Ser425) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data (P-peptide-+)

AKT1/3, Antibody (Cat# AAA852576)

Full Name
AKT1/3 (Phospho-Tyr437/434) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB) (Western blot All lanes: Peptidyl-prolyl cis-trans isomerase FKBP1A antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 12 kDa Observed band size: 22 kDa Additional bands at: 36 kDa. We are unsure as to the identity of these extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)

Peptidyl-prolyl cis-trans isomerase FKBP1A, Polyclonal Antibody (Cat# AAA715055)

Full Name
Rabbit anti-human Peptidyl-prolyl cis-trans isomerase FKBP1A polyclonal Antibody, HRP conjugated
Gene Names
FKBP1A; FKBP1; PKC12; PKCI2; FKBP12; PPIASE; FKBP-12; FKBP-1A
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg

Phosphorylation P38 Mitogen-activated Protein Kinase, ELISA Kit (Cat# AAA727814)

Full Name
Guinea pig Phosphorylation P38 Mitogen-activated Protein Kinase ELISA Kit
Reactivity
Guinea Pig
Pricing
$475/48-Strip-Wells | $680/96-Strip-Wells | $3,010/5x96-Strip-Wells | $5,675/10x96-Strip-Wells

Sphingosine Kinase 1, Mouse, Peptide (Cat# AAA396442)

Full Name
Sphingosine Kinase 1, Mouse
Gene Names
Sphk1; Sk1; Spk1; 1110006G24Rik
Applications
Antibody Function Blocking
Pricing
$240/0.05 mg | $925/5x0.05 mg
Western Blot (WB) (Western Blot analysis of extracts from HT29 cells, untreated or treated with UV using Ephrin-B2 (Phospho-Tyr330) antibody)

Ephrin-B2, Polyclonal Antibody (Cat# AAA835711)

Full Name
Ephrin-B2 antibody
Gene Names
EFNB2; HTKL; EPLG5; Htk-L; LERK5
Applications
WB
Purity
Ephrin-B2 antibody was purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg

Ikk alpha, beta, Polyclonal Antibody (Cat# AAA628611)

Full Name
Ikk alpha, beta (IkappaB Kinase alpha, beta, IKKa,b)
Gene Names
IKBKB; IKK2; IKKB; NFKBIKB; IKK-beta
Reactivity
Human, Mouse, Rat
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Testing Data (Peptide-+)

TNNI3, Antibody (Cat# AAA859575)

Full Name
TNNI3 (Ab-142) Antibody
Gene Names
TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC) (P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using CDC25C (Phospho-Ser198) antibody.)

CDC25C, Antibody (Cat# AAA855083)

Full Name
CDC25C (Phospho-Ser198) Antibody
Gene Names
CDC25C; CDC25; PPP1R60
Reactivity
Human
Applications
IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB) (ATP5H antibody (MBS536460) used at 0.2-1 ug/ml to detect target protein.)

ATP5H, Polyclonal Antibody (Cat# AAA536460)

Full Name
ATP5H antibody
Gene Names
ATP5H; ATPQ
Applications
WB
Pricing
$605/0.1 mL | $2,710/5x0.1 mL
Immunohistochemistry (IHC) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Rb (phospho-Ser780) antibody.)

Rb, Antibody (Cat# AAA857601)

Full Name
Rb (Phospho-Ser780) Antibody
Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC) (Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Ab-45) antibody.)

4E-BP1, Antibody (Cat# AAA8510051)

Full Name
4E-BP1 (Ab-45) Antibody
Gene Names
EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB) (Western blot detection of CSK in C6 and Jurkat cell lysates using CSK mouse mAb (1:1000 diluted).Predicted band size:50KDa.Observed band size:50KDa.)

CSK, Monoclonal Antibody (Cat# AAA475092)

Full Name
Anti-CSK Mouse mAb
Reactivity
Human, Rat
Applications
WB, ICC
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
Western Blot (WB) (Western blot All lanes: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 11.7 kDa Observed band size: 40 kDa Additional bands at: 80kDa. We are unsure as to the identity of these extra bands. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)

NADH dehydrogenase [ubiquinone] iron-sulfur protein 5, Polyclonal Antibody (Cat# AAA715385)

Full Name
Rabbit anti-human NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 polyclonal Antibody, HRP conjugated
Gene Names
NDUFS5; CI15K; CI-15k
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB) (Western blot All lanes: Tumor necrosis factor ligand superfamily member 14 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 26 kDa Observed band size: 45 kDa Additional bands at:60 kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)

Tumor necrosis factor ligand superfamily member 14, Polyclonal Antibody (Cat# AAA715853)

Full Name
Rabbit anti-human Tumor necrosis factor ligand superfamily member 14 polyclonal Antibody, FITC
Gene Names
TNFSF14; LTg; TR2; CD258; HVEML; LIGHT; TNLG1D
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Dot Blot (DB) (Dot blot analysis of anti-Phospho-HIPK2-pY361 Antibody (MBS9211178) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Phospho-HIPK2 (Y361), Polyclonal Antibody (Cat# AAA9211178)

Full Name
Phospho-HIPK2 (Y361) Antibody
Gene Names
HIPK2; PRO0593
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
DB, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot analysis of extracts from COS cells, treated with calyculinA (50ng/ml, 30mins), using Leptin Receptor (Ab-1141) antibody.)

Leptin Receptor, Antibody (Cat# AAA859188)

Full Name
Leptin Receptor (Ab-1141) Antibody
Gene Names
LEPR; OBR; OB-R; CD295; LEP-R; LEPRD
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB) (Western blot analysis of extracts from 293 cells, using HP1 gamma (Ab-93) antibody.)

HP1 gamma, Antibody (Cat# AAA852908)

Full Name
HP1 gamma (Ab-93) Antibody
Gene Names
CBX3; HECH; HP1-GAMMA; HP1Hs-gamma
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)

AKT1, Blocking Peptide (Cat# AAA9218772)

Full Name
AKT1 Antibody (C-term T450) Blocking peptide
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Western Blot (WB) (Western Blot analysis of extracts from HUVEC cells using Integrin beta3 (Phospho-Tyr785) antibody and the same antibody preincubated with blocking peptide.)

Integrin beta 3, Polyclonal Antibody (Cat# AAA837519)

Full Name
Integrin beta 3 antibody
Gene Names
ITGB3; GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16
Applications
WB
Purity
Integrin beta 3 antibody was purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
Western Blot (WB) (Western blot analysis of extracts from A549 cells, using AKT1/3 antibody)

AKT1/3, Polyclonal Antibody (Cat# AAA839554)

Full Name
AKT1/3 antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Applications
WB, EIA
Pricing
$405/0.1 mg | $1,805/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts from HUVEC cells, treated with EGF (200ng/ml, 30mins), using HER3/ErbB3 (Phospho-Tyr1222) antibody.)

HER3, Antibody (Cat# AAA859830)

Full Name
HER3 (Phospho-Tyr1222) Antibody
Gene Names
ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
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