Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB) (All lanes: Mouse anti-human ENO2 monoclonal antibody at 1ug/mlLane 1:Hela cell lysateLane 2:Recombinant Human Gamma-enolase(ENO2) at 10ugLane 3:U251 cell lysateSecondary Goat polyclonal to Mouse IgG at 1/3000 dilutionPredicted band size: 43,48 kDObserved band size: 48 kDAdditional bands at: 100kDa.)
Immunohistochemistry (IHC) (Immunohistochemical of paraffin-embedded human small intestine using MBS1499580 at dilution of 1:200)
Immunohistochemistry (IHC) (Immunohistochemical of paraffin-embedded human pancreas using MBS1499580 at dilution of 1:200)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded Rat Heart stained with NSE gamma Monoclonal Antibody (ENO2/1462).)
SDS-Page (SDS-PAGE Analysis Purified NSE gamma Mouse Monoclonal Antibody (ENO2/1462). Confirmation of Integrity and Purity of Antibody. )
Testing Data (Analysis of Protein Array containing more than 19, 000 full-length human proteins using NSE gamma (ENO2) Mouse Monoclonal Antibody (ENO2/1462) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. )
Western Blot (WB) (Western Blot analysis of various cells using Enolase Polyclonal Antibody at dilution of 1:2000.)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded Rat Heart stained with Formalin-fixed, paraffin-embedded Rat Heart stained with NSE gamma Monoclonal Antibody (ENO2/1375).)
Western Blot (WB) (Western blot detection of Enolase-2 in SHSY-5Y and U87-MG cell lysates using Enolase-2 mouse mAb (1:1000 diluted).Predicted band size:47KDa.Observed band size:47KDa.)
Western Blot (WB) (Western blot detection of Enolase-2 in Rat Brain and Mouse Brain lysates using Enolase-2 mouse mAb (1:1000 diluted).Predicted band size:47KDa.Observed band size:47KDa.)
Testing Data (Observed Enolase-2 levels in CHO-K1 cell lysates transfected with Enolase-2 at different dilution.)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (SPM347).)
Immunohistochemistry (IHC) (MBS9601642 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunofluorescene (IF) (MBS9601642 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB) (Western blot analysis of NSE expression in HepG2 cells)
Western Blot (WB) (Western blot analysis of Hepg2 whole cell lysates, using ENO2 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Immunofluorescene (IF) (MBS9607844 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB) (Western Blot Lane1: Human Hepg2 Cells Lane2: Human Hela Cells Primary Ab: 2ug/mL Rabbit Anti-Human NSE Ab Second Ab: 1:5000 Dilution of HRP-Linked Rabbit Anti-Mouse IgG Ab )
Western Blot (WB) (Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC) (DAB staining on IHC-P Samples:Human Lung Cancer Tissue))
Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples:Human Pancreas Cancer Tissue))
Immunohistochemistry (IHC) (DAB staining on IHC-P Samples:Human Breast Cancer Tissue))
Western Blot (WB) (Western blot analysis on HepG2 cell lysate using NSE Antibody. The lane on the left is treated with the antigen-specific peptide.)
Immunofluorescene (IF) (MBS9600160 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunohistochemistry (IHC) (MBS9600160 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ENO2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat brain using ENO2 antibody at dilution of 1:100 (40x lens).)
Western Blot (WB) (Western blot detection of Enolase-2 in SHSY-5Y and U87-MG cell lysates using Enolase-2 mouse mAb (1:500 diluted).Predicted band size:47KDa.Observed band size:47KDa.)
Western Blot (WB) (Western blot detection of Enolase-2 in Rat Brain and Mouse Brain lysates using Enolase-2 mouse mAb (1:500 diluted).Predicted band size:47KDa.Observed band size:47KDa.)
Testing Data (Observed Enolase-2 levels in CHO-K1 cell lysates transfected with Enolase-2 at different dilution.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human brain tissue using NSE antibody.)
Immunofluorescence (IF) (Immunofluorescence analysis of A549 cells, using NSE antibody.)
Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, using NSE antibody.)
Western Blot (WB) (Western blot analysis of extracts from Hela cells (Lane 2), colo cells(Lane 3) ¬HepG2 cells(Lane 4) and 293 cells (Lane 5), using NSE antiobdy. The lane on the left is treated with synthesized peptide.)
Western Blot (WB) (Western blot analysis of NR2F6 expression in HepG2 (A), rat liver (B) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of NR2F6 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF) (Immunofluorescent analysis of NR2F6 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human stomach cancer using ENO2 Polyclonal Antibody at dilution of 1:300.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded mouse brain using ENO2 Polyclonal Antibody at dilution of 1:400.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded rat brain using ENO2 Polyclonal Antibody at dilution of 1:400.)
Western Blot (WB) (All Lanes Mouse anti-human ENO2 monoclonal antibody at 1ug/ml)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human small intestine using at dilution of 1:200.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human pancreas using at dilution of 1:200.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ENO2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Immunofluorescence (IF) (Immunofluorescence analysis of A549 cells, using NSE Antibody. The picture on the right is blocked with the synthesized peptide.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NSE Antibody. The picture on the right is blocked with the synthesized peptide.)
Western Blot (WB) (Western blot analysis of lysates from HepG2 cells, using NSE Antibody. The lane on the right is blocked with the synthesized peptide.)
Western Blot (WB) (Dilution: WB - 1:1000All lanes: Anti-ENOG at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: CEM whole cell lysate Lane 3: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC) (Dilution: IHC-P - 1:10~50ENOG Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjmugation of the secondary antibody and DAB staining.This data demonstrates the use of ENOG Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)